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On One Page

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Product(s) |

Expression|

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Standard Name	sufB
Gene Synonym(s)	ECK1679, b1683, JW5273, ynhE^[1], ynhE
Product Desc.	component of SufB-SufC-SufD cysteine desulfurase (SufS) activator complex^[2]^[3]; Component of SufB-SufC-SufD cysteine desulfurase (SufS) activator complex^[3] Complexed with SufC and SufD^[4]
Product Synonyms(s)	component of SufBCD complex^[1], YnhE^[2]^[1], SufB^[2]^[1], B1683^[2]^[1] , ECK1679, JW5273, ynhE, b1683
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): sufABCDSE^[2], suf
Regulation/Activity
Quick Links
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Notes

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SufBCD increases SufSE cysteine desulfurase activity and involved in iron acquisition.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	sufB
Mnemonic	Sulfur
Synonyms	ECK1679, b1683, JW5273, ynhE^[1], ynhE
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	37.95 minutes	MG1655: 1762033..1760546 <gbrowseImage> name=NC_000913:1760546..1762033 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1741276..1739789 <gbrowseImage> name=NC_012967:1739789..1741276 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1652605..1654092 <gbrowseImage> name=NC_012759:1652605..1654092 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1765723..1764236 <gbrowseImage> name=NC_007779:1764236..1765723 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1852604..1851117 <gbrowseImage> name=NC_010473:1851117..1852604 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
ΔsufB (Keio:JW5273)	deletion	deletion			PMID:16738554	Shigen CGSC11285^[5]
ΔynhE::kan	deletion		Biolog:respiration	unable to respire a-D-Glucose-1-phosphate	PMID:16095938
ΔynhE::kan	deletion		Biolog:respiration	unable to respire a-D-Glucose-6-phosphate	PMID:16095938
ΔynhE::kan	deletion		Biolog:respiration	unable to respire a-Ketobutyrate	PMID:16095938
ΔsufB758::kan					PMID:16738554	CGSC:103058
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5273	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCTCGTAATACTGAAGCAAC Primer 2:CCTCCGACGCTGTGTTCAAGACT
15G10	Kohara Phage	Genobase	PMID:3038334
zdd-235::Tn10	Linked marker	CAG18461 = CGSC7378^[5]	est. P1 cotransduction: % ^[6] Synonyms:zdc-235::Tn10
zdi-925::Tn10	Linked marker	CAG12151 = CGSC7385^[5]	est. P1 cotransduction: 56% ^[6] Synonyms:zdh-925::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6909	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13965	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003498	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945753	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3723	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005621	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	SufB
Synonyms	component of SufBCD complex^[1], YnhE^[2]^[1], SufB^[2]^[1], B1683^[2]^[1] , ECK1679, JW5273, ynhE, b1683
Product description	component of SufB-SufC-SufD cysteine desulfurase (SufS) activator complex^[2]^[3]; Component of SufB-SufC-SufD cysteine desulfurase (SufS) activator complex^[3] Complexed with SufC and SufD^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005515	protein binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000825	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005515	protein binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010231	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005515	protein binding	PMID:15690043	IPI: Inferred from Physical Interaction	UniProtKB:P77689	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005515	protein binding	PMID:17350958	IPI: Inferred from Physical Interaction	UniProtKB:P76194	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016226	iron-sulfur cluster assembly	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000825	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016226	iron-sulfur cluster assembly	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010231	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016226	iron-sulfur cluster assembly	PMID:12089140	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc (v14.0)	complete
	GO:0051539	4 iron, 4 sulfur cluster binding	PMID:17350958	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of SufB-SufC-SufD cysteine desulfurase (SufS) activator complex	could be indirect
Protein	sufC		PMID:15690043	Experiment(s):EBI-889086, EBI-889106
Protein	sufC		PMID:19402753	MALDI(Z-score):17.053160
Protein	sufD		PMID:19402753	LCMS(ID Probability):99.6 MALDI(Z-score):38.795348
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MWLWRKLWGI GGTMSRNTEA TDDVKTWTGG PLNYKEGFFT QLATDELAKG INEEVVRAIS AKRNEPEWML EFRLNAYRAW LEMEEPHWLK AHYDKLNYQD YSYYSAPSCG NCDDTCASEP GAVQQTGANA FLSKEVEAAF EQLGVPVREG KEVAVDAIFD SVSVATTYRE KLAEQGIIFC SFGEAIHDHP ELVRKYLGTV VPGNDNFFAA LNAAVASDGT FIYVPKGVRC PMELSTYFRI NAEKTGQFER TILVADEDSY VSYIEGCSAP VRDSYQLHAA VVEVIIHKNA EVKYSTVQNW FPGDNNTGGI LNFVTKRALC EGENSKMSWT QSETGSAITW KYPSCILRGD NSIGEFYSVA LTSGHQQADT GTKMIHIGKN TKSTIISKGI SAGHSQNSYR GLVKIMPTAT NARNFTQCDS MLIGANCGAH TFPYVECRNN SAQLEHEATT SRIGEDQLFY CLQRGISEED AISMIVNGFC KDVFSELPLE FAVEAQKLLA ISLEHSVG
Length	508
Mol. Wt	56.33 kDa
pI	5.1 (calculated)
Extinction coefficient	84,800 - 86,425 (calc based on 20 Y, 10 W, and 13 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	229..466	PF01458 Uncharacterized protein family (UPF0051)		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=sufB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111315	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945753	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005621	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P77522	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6909	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13965	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945753	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003498	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3723	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	75	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	143	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	71	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
sufABCDSE
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1762013..1762053 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1683 (EcoliWiki Page)	NCBI GEO profiles for sufB
microarray	GenExpDB:b1683 (EcoliWiki Page)	Summary of data for sufB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to sufB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6909	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3723	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1683
EcoGene	EcoGene:EG13965	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003498	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005621	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT4G04770 (score: 1.000; bootstrap: 100%) AT5G44316 (score: 0.285)	From Inparanoid:20070104
Shigella flexneri	YNHE	From SHIGELLACYC
E. coli O157	YNHE	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:101960
Pfam (EcoliWiki Page)	PF01458 Uncharacterized protein family (UPF0051)
EcoCyc	EcoCyc:G6909	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13965	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003498	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3723	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005621	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

sufB:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to sufB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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