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Expression|

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Standard Name	stpA
Gene Synonym(s)	ECK2663, b2669, JW2644, hnsB, rsv^[1], rsv
Product Desc.	H-NS-like DNA-binding protein with RNA chaperone activity^[2]^[3] RNA chaperone and DNA-binding protein; suppresses T4 td mutant^[4]; modulates micF stability; forms heteromers with, and stabilzed against proteolysis by, the paralogous H-NS protein; transcriptionally repressed by H-NS^[5]
Product Synonyms(s)	DNA binding protein, nucleoid-associated^[1], B2669^[2]^[1], HnsB^[2]^[1], StpA^[2]^[1] , ECK2663, hnsB, JW2644, rsv, b2669
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): stpA^[2]
Regulation/Activity
Quick Links
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Notes

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Nucleoid-associated protein.

StpA binds preferably unstructured RNA weakly, with mainly electrostatic interactions that promote RNA folding (Mayer et al. 2007).^[5]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	stpA
Mnemonic	Suppressor of T4 td mutant^[4]
Synonyms	ECK2663, b2669, JW2644, hnsB, rsv^[1], rsv
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	60.27 minutes	MG1655: 2796517..2796113 <gbrowseImage> name=NC_000913:2796113..2796517 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2693248..2692844 <gbrowseImage> name=NC_012967:2692844..2693248 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2681925..2682329 <gbrowseImage> name=NC_012759:2681925..2682329 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2797151..2796747 <gbrowseImage> name=NC_007779:2796747..2797151 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2888282..2887878 <gbrowseImage> name=NC_010473:2887878..2888282 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2796116	Edman degradation	PMID:7493324
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
ΔstpA (Keio:JW2644)	deletion	deletion			PMID:16738554	Shigen CGSC10083^[6]
ΔstpA::kan	deletion		Biolog:respiration	unable to respire a-Ketobutyrate	PMID:16095938
ΔstpA::kan	deletion		Biolog:respiration	unable to respire a-Ketoglutarate	PMID:16095938
ΔstpA750::kan					PMID:16738554	CGSC:103048
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2644	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCCGTAATGTTACAAAGTTT Primer 2:CCGATCAGGAAATCGTCGAGAGA
10H9	Kohara Phage	Genobase	PMID:3038334
8G10	Kohara Phage	Genobase	PMID:3038334
pheA18::Tn10	Linked marker	CAG12158 = CGSC7421^[6]	est. P1 cotransduction: 5% ^[7]
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[6]	est. P1 cotransduction: 32% ^[7] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11554	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11554	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001507	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947130	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1515	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008788	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	StpA
Synonyms	DNA binding protein, nucleoid-associated^[1], B2669^[2]^[1], HnsB^[2]^[1], StpA^[2]^[1] , ECK2663, hnsB, JW2644, rsv, b2669
Product description	H-NS-like DNA-binding protein with RNA chaperone activity^[2]^[3] RNA chaperone and DNA-binding protein; suppresses T4 td mutant^[4]; modulates micF stability; forms heteromers with, and stabilzed against proteolysis by, the paralogous H-NS protein; transcriptionally repressed by H-NS^[5]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001801	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005622	intracellular	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001801	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001801	P	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	PMID:10551881	IDA: Inferred from Direct Assay		F		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rplB	PMID:16606699	Experiment(s):EBI-1143623
Protein	htpG	PMID:16606699	Experiment(s):EBI-1143623
Protein	ycgE	PMID:16606699	Experiment(s):EBI-1143623
Protein	slyD	PMID:16606699	Experiment(s):EBI-1143623
Protein	rpsD	PMID:16606699	Experiment(s):EBI-1143623
Protein	prs	PMID:16606699	Experiment(s):EBI-1143623
Protein	talB	PMID:19402753	MALDI(Z-score):22.859500
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSVMLQSLNN IRTLRAMARE FSIDVLEEML EKFRVVTKER REEEEQQQRE LAERQEKIST WLELMKADGI NPEELLGNSS AAAPRAGKKR QPRPAKYKFT DVNGETKTWT GQGRTPKPIA QALAEGKSLD DFLI
Length	134
Mol. Wt	15.347 kDa
pI	8.6 (calculated)
Extinction coefficient	12,490 (calc based on 1 Y, 2 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	24..129	PF00816 H-NS histone family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=stpA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130583	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947130	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008788	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ACG1	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11554	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11554	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947130	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001507	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1515	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.11E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	28631	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	7682	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	6284	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
stpA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2796497..2796537 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2669 (EcoliWiki Page)	NCBI GEO profiles for stpA
microarray	GenExpDB:b2669 (EcoliWiki Page)	Summary of data for stpA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2796448..2797205) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ10; Well:F2^[8]
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Notes

Accessions Related to stpA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11554	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1515	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2669
EcoGene	EcoGene:EG11554	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001507	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008788	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	STPA	From SHIGELLACYC
E. coli O157	STPA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00816 H-NS histone family
Superfamily (EcoliWiki Page)	SUPERFAMILY:81273
Superfamily (EcoliWiki Page)	SUPERFAMILY:81273
EcoCyc	EcoCyc:EG11554	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11554	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001507	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1515	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008788	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 Zhang, A & Belfort, M (1992) Nucleotide sequence of a newly-identified Escherichia coli gene, stpA, encoding an H-NS-like protein. Nucleic Acids Res. 20 6735 PubMed
↑ ^5.0 ^5.1 ^5.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^6.0 ^6.1 ^6.2 CGSC: The Coli Genetics Stock Center
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

stpA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to stpA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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