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Expression|

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Standard Name	slt
Gene Synonym(s)	ECK4384, b4392, JW4355, sltY^[1], sltY
Product Desc.	soluble lytic murein transglycosylase^[2]^[3] Soluble lytic murein transglycosylase Slt70, major autolysin, periplasmic^[4]
Product Synonyms(s)	lytic murein transglycosylase, soluble ^[1], B4392 ^[2]^[1], SltY ^[2]^[1], Slt ^[2]^[1], Slt70 ^[2]^[1] , ECK4384, JW4355, sltY, b4392
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): slt^[2]
Regulation/Activity
Quick Links
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Notes

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Slt cleaves the glycosidic bonds between the N-acetylmuramic acid and N-acetylglucosamine residues in murein. Slt has a C-terminal lysozyme-like domain.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	slt
Mnemonic	Soluble lytic transglycosylase
Synonyms	ECK4384, b4392, JW4355, sltY^[1], sltY
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	99.76 minutes	MG1655: 4628756..4630693 <gbrowseImage> name=NC_000913:4628756..4630693 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4618946..4620883 <gbrowseImage> name=NC_012967:4618946..4620883 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4567240..4569177 <gbrowseImage> name=NC_012759:4567240..4569177 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4635413..4637350 <gbrowseImage> name=NC_007779:4635413..4637350 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4675218..4677155 <gbrowseImage> name=NC_010473:4675218..4677155 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	4628837	Edman degradation	PMID:1938883
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
Δslt (Keio:JW4355)	deletion	deletion		PMID:16738554	Shigen CGSC11109^[5]
sltE505Q		E505Q	Inactivates the enzyme			seeded from UniProt:P0AGC3
Δslt-788::kan				PMID:16738554	CGSC:102962
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4355	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGAAAAAGCCAAACAAGTTAC Primer 2:CCGTAACGACGTCCCCATTCCGT
12A4	Kohara Phage	Genobase	PMID:3038334
15A5	Kohara Phage	Genobase	PMID:3038334
mdoB202::Tn10	Linked marker	CAG18430 = CGSC7488^[5]	est. P1 cotransduction: 24% ^[6] Synonyms:zjj-202::Tn10, zji-202::Tn10
thrA34::Tn10	Linked marker	CAG18442 = CGSC7320^[5]	est. P1 cotransduction: 69% ^[6] Synonyms:thr-34::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10950	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10950	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000939	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948908	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0943	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014404	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Slt
Synonyms	lytic murein transglycosylase, soluble ^[1], B4392 ^[2]^[1], SltY ^[2]^[1], Slt ^[2]^[1], Slt70 ^[2]^[1] , ECK4384, JW4355, sltY, b4392
Product description	soluble lytic murein transglycosylase^[2]^[3] Soluble lytic murein transglycosylase Slt70, major autolysin, periplasmic^[4]
EC number (for enzymes)	3.2.1.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0030288	outer membrane-bounded periplasmic space	PMID:1938883	IDA: Inferred from Direct Assay		C	Seeded from Riley et al 2006 ^[1].	complete
	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008939	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012289	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008933	lytic transglycosylase activity	PMID:1938883	IDA: Inferred from Direct Assay		F		complete
	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016026	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016829	lyase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0456	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016837	carbon-oxygen lyase activity, acting on polysaccharides	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.2.2	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
Periplasm	PMID:1938883	EchoLocation:slt
periplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MYLHLEDALV EKAKQVTWRL LAAGVCLLTV SSVARADSLD EQRSRYAQIK QAWDNRQMDV VEQMMPGLKD YPLYPYLEYR QITDDLMNQP AVTVTNFVRA NPTLPPARTL QSRFVNELAR REDWRGLLAF SPEKPGTTEA QCNYYYAKWN TGQSEEAWQG AKELWLTGKS QPNACDKLFS VWRASGKQDP LAYLERIRLA MKAGNTGLVT VLAGQMPADY QTIASAIISL ANNPNTVLTF ARTTGATDFT RQMAAVAFAS VARQDAENAR LMIPSLAQAQ QLNEDQIQEL RDIVAWRLMG NDVTDEQAKW RDDAIMRSQS TSLIERRVRM ALGTGDRRGL NTWLARLPME AKEKDEWRYW QADLLLERGR EAEAKEILHQ LMQQRGFYPM VAAQRIGEEY ELKIDKAPQN VDSALTQGPE MARVRELMYW NLDNTARSEW ANLVKSKSKT EQAQLARYAF NNQWWDLSVQ ATIAGKLWDH LEERFPLAYN DLFKRYTSGK EIPQSYAMAI ARQESAWNPK VKSPVGASGL MQIMPGTATH TVKMFSIPGY SSPGQLLDPE TNINIGTSYL QYVYQQFGNN RIFSSAAYNA GPGRVRTWLG NSAGRIDAVA FVESIPFSET RGYVKNVLAY DAYYRYFMGD KPTLMSATEW GRRY
Length	654
Mol. Wt	74.408 kDa
pI	8.8 (calculated)
Extinction coefficient	154,700 - 155,075 (calc based on 30 Y, 20 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-27	UniProt Manual:Signal Peptides	UniProt:P0AGC3
Domain	482..602	PF01464 Transglycosylase SLT domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=slt taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111747	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948908	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014404	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AGC3	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10950	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10950	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948908	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000939	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0943	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	270	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	204	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	195	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
slt
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4628736..4628776 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4392 (EcoliWiki Page)	NCBI GEO profiles for slt
microarray	GenExpDB:b4392 (EcoliWiki Page)	Summary of data for slt from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4627994..4628320) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ14; Well:C1^[7]
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Notes

Accessions Related to slt Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10950	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0943	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4392
EcoGene	EcoGene:EG10950	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000939	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014404	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	SLT	From SHIGELLACYC
E. coli O157	SLT	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:48435
Panther (EcoliWiki Page)	PTHR21666:SF40
Superfamily (EcoliWiki Page)	SUPERFAMILY:53955
Pfam (EcoliWiki Page)	PF01464 Transglycosylase SLT domain
EcoCyc	EcoCyc:EG10950	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10950	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000939	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0943	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014404	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

slt:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to slt Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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