slt:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Slt

Synonyms

lytic murein transglycosylase, soluble [1], B4392 [2][1], SltY [2][1], Slt [2][1], Slt70 [2][1] , ECK4384, JW4355, sltY, b4392

Product description

soluble lytic murein transglycosylase[2][3]

Soluble lytic murein transglycosylase Slt70, major autolysin, periplasmic[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0030288

outer membrane-bounded periplasmic space

PMID:1938883[5]

IDA: Inferred from Direct Assay

C

Seeded from Riley et al 2006 [1].

complete

GO:0004553

hydrolase activity, hydrolyzing O-glycosyl compounds

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008939

F

Seeded from EcoCyc (v14.0)

complete

GO:0004553

hydrolase activity, hydrolyzing O-glycosyl compounds

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012289

F

Seeded from EcoCyc (v14.0)

complete

GO:0008933

lytic transglycosylase activity

PMID:1938883[5]

IDA: Inferred from Direct Assay

F

complete

GO:0004553

hydrolase activity, hydrolyzing O-glycosyl compounds

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016026

F

Seeded from EcoCyc (v14.0)

complete

GO:0016829

lyase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0456

F

Seeded from EcoCyc (v14.0)

complete

GO:0016837

carbon-oxygen lyase activity, acting on polysaccharides

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:4.2.2

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Periplasm

PMID:1938883[5]

EchoLocation:slt

periplasm


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MYLHLEDALV EKAKQVTWRL LAAGVCLLTV SSVARADSLD EQRSRYAQIK QAWDNRQMDV
VEQMMPGLKD YPLYPYLEYR QITDDLMNQP AVTVTNFVRA NPTLPPARTL QSRFVNELAR
REDWRGLLAF SPEKPGTTEA QCNYYYAKWN TGQSEEAWQG AKELWLTGKS QPNACDKLFS
VWRASGKQDP LAYLERIRLA MKAGNTGLVT VLAGQMPADY QTIASAIISL ANNPNTVLTF
ARTTGATDFT RQMAAVAFAS VARQDAENAR LMIPSLAQAQ QLNEDQIQEL RDIVAWRLMG
NDVTDEQAKW RDDAIMRSQS TSLIERRVRM ALGTGDRRGL NTWLARLPME AKEKDEWRYW
QADLLLERGR EAEAKEILHQ LMQQRGFYPM VAAQRIGEEY ELKIDKAPQN VDSALTQGPE
MARVRELMYW NLDNTARSEW ANLVKSKSKT EQAQLARYAF NNQWWDLSVQ ATIAGKLWDH
LEERFPLAYN DLFKRYTSGK EIPQSYAMAI ARQESAWNPK VKSPVGASGL MQIMPGTATH
TVKMFSIPGY SSPGQLLDPE TNINIGTSYL QYVYQQFGNN RIFSSAAYNA GPGRVRTWLG
NSAGRIDAVA FVESIPFSET RGYVKNVLAY DAYYRYFMGD KPTLMSATEW GRRY
Length

654

Mol. Wt

74.408 kDa

pI

8.8 (calculated)

Extinction coefficient

154,700 - 155,075 (calc based on 30 Y, 20 W, and 3 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

motif

1-27

UniProt Manual:Signal Peptides

UniProt:P0AGC3

Domain

482..602

PF01464 Transglycosylase SLT domain

PMID:19920124[6]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=slt taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111747

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948908

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0014404

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AGC3

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10950

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10950

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948908

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000939

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0943

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 Engel, H et al. (1991) Murein-metabolizing enzymes from Escherichia coli: sequence analysis and controlled overexpression of the slt gene, which encodes the soluble lytic transglycosylase. J. Bacteriol. 173 6773-82 PubMed
  6. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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