sdaA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
sdaA |
|---|---|
| Mnemonic |
Serine deaminase |
| Synonyms |
ECK1812, b1814, JW1803[1], JW1803 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
40.84 minutes |
MG1655: 1894956..1896320 |
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NC_012967: 1875511..1876875 |
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NC_012759: 1787015..1788379 |
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W3110 |
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W3110: 1898646..1900010 |
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DH10B: 1985527..1986891 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
1894956 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔsdaA (Keio:JW1803) |
deletion |
deletion |
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sdaA::Tn5KAN-2 (FB20458) |
Insertion at nt 899 in Plus orientation |
does not contain pKD46 | |||||
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sdaA::Tn5KAN-2 (FB20459) |
Insertion at nt 899 in Plus orientation |
contains pKD46 | |||||
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ΔsdaA738::kan |
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sdaA(del) sdaB(del) tdcG(del) |
deletion |
deletion |
Colony Morphology |
When triple mutant is grown in liquid glucose minimal medium supplemented with casamino acids produced misshaped cells and finally lysed, figure 2. |
Parent Strain: MEW1 Experimental triple mutant strain: MEW999 |
Growth defect was due to the loss of L-serine deaminase and was proven by restoring wildtype growth even with casamino acids via strain with S-adenosylmethionine (SAM) plasmid insertion. MEW999psamT | |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1803 |
Plasmid clone |
Status:Clone OK Primer 1:GCCATTAGTCTATTCGACATGTT Primer 2:CCGTCACACTGGACTTTGATTGC | |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 39% [9] | ||
|
Linked marker |
est. P1 cotransduction: 24% [9] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Su, H et al. (1993) Use of gene fusions of the structural gene sdaA to purify L-serine deaminase 1 from Escherichia coli K-12. Eur. J. Biochem. 211 521-7 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Zhang, X & Newman, E (2008) Deficiency in l-serine deaminase results in abnormal growth and cell division of Escherichia coli K-12. Mol. Microbiol. 69 870-81 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
