rhsE:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

rhsE

Gene Synonym(s)

ECK1450, b1456, JW1451[1], JW1451

Product Desc.

RhsE protein in rhs element[2][3]

Product Synonyms(s)

rhsE element core protein RshE[1], B1456[2][1], RhsE[2][1] , ECK1450, JW1451, b1456

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): rhsE[2]

Regulation/Activity
Quick Links

porteco.png regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rhsE

Mnemonic
Synonyms

ECK1450, b1456, JW1451[1], JW1451

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

32.89 minutes 

MG1655: 1525914..1527962
<gbrowseImage> name=NC_000913:1525914..1527962 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1500277..1504500
<gbrowseImage> name=NC_012967:1500277..1504500 source=REL606 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1529604..1531652
<gbrowseImage> name=NC_007779:1529604..1531652 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1616509..1618557
<gbrowseImage> name=NC_010473:1616509..1618557 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrhsE (Keio:JW1451)

deletion

deletion

PMID:16738554

Shigen
CGSC9264[4]

rhsE::Tn5KAN-2 (FB20324)

Insertion at nt 1475 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20324

contains pKD46

rhsE::Tn5KAN-2 (FB20325)

Insertion at nt 1475 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20325

does not contain pKD46

ΔrhsE749::kan

PMID:16738554

CGSC:102704


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1451

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCGACTCGCTTAACCACCCT

Primer 2:CCATAATTTGAAGCATCTGAACT

8B7

Kohara Phage

Genobase

PMID:3038334

6D4

Kohara Phage

Genobase

PMID:3038334

1G4

Kohara Phage

Genobase

PMID:3038334

trg-2::Tn10

Linked marker

CAG12026 = CGSC7376[4]

est. P1 cotransduction: 28% [5]

zdd-235::Tn10

Linked marker

CAG18461 = CGSC7378[4]

est. P1 cotransduction: 71% [5]
Synonyms:zdc-235::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946026

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000008

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RhsE

Synonyms

rhsE element core protein RshE[1], B1456[2][1], RhsE[2][1] , ECK1450, JW1451, b1456

Product description

RhsE protein in rhs element[2][3]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rpmE

PMID:16606699

Experiment(s):EBI-1136918

Protein

uup

PMID:16606699

Experiment(s):EBI-1136918

Protein

ybfA

PMID:16606699

Experiment(s):EBI-1136918

Protein

rhsC

PMID:16606699

Experiment(s):EBI-1136918

Protein

relE

PMID:16606699

Experiment(s):EBI-1136918

Protein

yeeT

PMID:16606699

Experiment(s):EBI-1136918

Protein

groL

PMID:16606699

Experiment(s):EBI-1136918, EBI-1136918

Protein

dnaK

PMID:16606699

Experiment(s):EBI-1136918, EBI-1136918

Protein

dnaE

PMID:16606699

Experiment(s):EBI-1136918

Protein

glnG

PMID:16606699

Experiment(s):EBI-1136918

Protein

tatE

PMID:16606699

Experiment(s):EBI-1136918

Protein

rpsT

PMID:16606699

Experiment(s):EBI-1136918

Protein

minE

PMID:16606699

Experiment(s):EBI-1136918

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MSTRLTTLSH TSEGHRVSVH YGYDDKGRLT GERQTVENPE TGELLWHHET GHAYNEQGLA
NRVTPDSLPP VEWLTYGSGY LAGMKLGGTP LLEFTRDRLH RETVRSFGSM AGSNAAYKLT
STYTPAGQLQ SQHLNSLVYD RDYGWNDNGD LVRISGPRQT REYGYSATGR LESVRTLAPD
LDIRIPYATD PAGNRLPDPE LHPDSTLTVW PDNRIAEDAH YVYRHDEYGR LTEKTDRIPA
GVIRTDDERT HHYHYDSQHR LVFYTRIQHG EPLVESRYLY DPLGRRMAKR VWRRERDLTG
WMSLSRKPEV TWYGWDGDRL TTVQTDTTRI QTVYEPGSFT PLIRVETENG EREKAQRRSL
AETLQQEGSE NGHGVVFPAE LVRLLDRLEE EIRADRVSSE SRAWLAQCGL TVEQLARQVE
PEYTPARKVH FYHCDHRGLP LALISEDGNT AWRGEYDEWG NQLNEENPHH LHQPYRLPGQ
QHDEESGLYY NRHRHYDPLQ GRYITPDPIG LRGGWNMYQY PLNPIQVIDP MGLDAIENMT
SGGLIYAVSG VPGLIAANSI TNSAYQFGYD MDAIVGGAHN GAADAMRHCY LMCRMTKTFG
STIADVIGKN HEAAGDRQGQ PAKERIMDLK NNTVGIACGD FSAKCSDACI EKYNTGQLFG
LDGIKADNPI KAKQGSSDAS NY
Length

682

Mol. Wt

77.141 kDa

pI

6.2 (calculated)

Extinction coefficient

122,620 - 123,495 (calc based on 38 Y, 12 W, and 7 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

429..469

PF03527 RHS protein

PMID:19920124

Domain

225..270

PF05593 RHS Repeat

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rhsE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GeneID:946026

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P24211

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946026

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000008

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

rhsE

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1525894..1525934 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1456 (EcoliWiki Page)

NCBI GEO profiles for rhsE

microarray

GenExpDB:b1456 (EcoliWiki Page)

Summary of data for rhsE from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (1525106..1525962) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ08; Well:A4[6]

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Notes

Accessions Related to rhsE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1456

RegulonDB

RegulonDB:ECK120000008

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Bos taurus

  • ENSBTAP00000009783 (score: 1.000; bootstrap: 100%)
  • ENSBTAP00000039755 (score: 0.704)
  • ENSBTAP00000036826 (score: 0.689)
  • ENSBTAP00000018431 (score: 0.501)
  • ENSBTAP00000034908 (score: 0.091)
  • YP_026224 (score: 1.000; bootstrap: 100%)
  • YP_026234 (score: 0.073)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000017541 (score: 1.000; bootstrap: 100%)
  • YP_026224 (score: 0.856)
  • YP_026234 (score: 0.086)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000009228 (score: 1.000; bootstrap: 100%)
  • ENSMODP00000005061 (score: 0.735)
  • ENSMODP00000005751 (score: 0.678)
  • ENSMODP00000018654 (score: 0.620)
  • YP_026224 (score: 1.000; bootstrap: 100%)
  • YP_026234 (score: 0.087)

From Inparanoid:20070104

Mus musculus

  • MGI:1345183 (score: 1.000; bootstrap: 100%)
  • MGI:2447063 (score: 0.698)
  • MGI:1345184 (score: 0.693)
  • MGI:1345185 (score: 0.609)
  • YP_026224 (score: 1.000; bootstrap: 100%)
  • YP_026234 (score: 0.080)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000003591 (score: 1.000; bootstrap: 69%)
  • YP_026224 (score: 0.848)

From Inparanoid:20070104

Shigella flexneri

RHS-LIKE

From SHIGELLACYC

E. coli O157

Z0272

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF03527 RHS protein

Pfam (EcoliWiki Page)

PF05593 RHS Repeat

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000008

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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