recJ:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

recJ

Gene Synonym(s)

ECK2887, b2892, JW2860[1], JW2860

Product Desc.

RecJ[2][3]

ssDNA 5'-3' exonuclease involved in recombination[4]

Product Synonyms(s)

ssDNA exonuclease, 5' --> 3'-specific[1], B2892[2][1], RecJ[2][1] , ECK2887, JW2860, b2892

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): dsbC-recJ-prfB[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Lovett and Kolodner (1989) N-terminally sequenced RecJ, but the native RBS was not used to direct translation, so it has been removed from the Verified Set. theless, the GTG start codon has been confirmed by mutational analysis in Haggerty and Lovett (1997).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

recJ

Mnemonic

Recombination

Synonyms

ECK2887, b2892, JW2860[1], JW2860

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

65.4 minutes 

MG1655: 3036128..3034395
<gbrowseImage> name=NC_000913:3034395..3036128 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2923859..2922126
<gbrowseImage> name=NC_012967:2922126..2923859 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2921543..2923276
<gbrowseImage> name=NC_012759:2921543..2923276 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3036762..3035029
<gbrowseImage> name=NC_007779:3035029..3036762 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3129998..3128265
<gbrowseImage> name=NC_010473:3128265..3129998 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrecJ (Keio:JW2860)

deletion

deletion

PMID:16738554

Shigen
CGSC10217[5]

recJ::Tn5KAN-I-SceI (FB20963)

Insertion at nt 1026 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20963

contains pKD46

recJ::Tn5KAN-I-SceI (FB20964)

Insertion at nt 1026 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20964

does not contain pKD46

recJ284::Tn10

Insertion of Tn10 (Tetracycline-resistance) at nucleotide 434 relative to GTG start codon

truncation

null phenotype, recombination deficient and UV-sensitive in recBC sbcA and recBC sbcBC backgrounds. Highly synergistic for UV-sensitivity, recombination deficiency and partial inviability with recBC.

[6]

CGSC

recJ77

G to A nucleotide 281

G to D amino acid 78

similar to recJ284, null

[6] [7]

recJ153 recJ154

GTG to GTA at start codon

None

Reduces expression of recJ gene, can be suppressed by mutations in initiation factor IF3 for translation

[8] [6] [9]

recJ153

CGSC:7801

recJ284::Tn10 (KD2238)

Resistant to

Mutation increases resistance to Thymineless Death (TLD). Figure 1.

PMID:2848620

CGSC:72003

Parent Strain: KD2197

ΔrecJ743::kan

PMID:16738554

CGSC:102628


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2860

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAACAACAGATACAACTTCG

Primer 2:CCAATTGGCCAGATATTGTCGAT

5B4

Kohara Phage

Genobase

PMID:3038334

22H4

Kohara Phage

Genobase

PMID:3038334

5E11

Kohara Phage

Genobase

PMID:3038334

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[5]

est. P1 cotransduction: % [10]

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[5]

est. P1 cotransduction: 9% [10]
Synonyms:zgd-210::Tn10, zgf-210::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10830

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10830

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000821

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947367

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0823

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009492

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RecJ

Synonyms

ssDNA exonuclease, 5' --> 3'-specific[1], B2892[2][1], RecJ[2][1] , ECK2887, JW2860, b2892

Product description

RecJ[2][3]

ssDNA 5'-3' exonuclease involved in recombination[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003156

F

Seeded from EcoCyc (v14.0)

complete

GO:0004518

nuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0540

F

Seeded from EcoCyc (v14.0)

complete

GO:0004527

exonuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0269

F

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004610

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004610

P

Seeded from EcoCyc (v14.0)

complete

GO:0008409

5'-3' exonuclease activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004610

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001667

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0030145

manganese ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001667

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

flhA

PMID:16606699

Experiment(s):EBI-1144203

Protein

aceE

PMID:15690043

Experiment(s):EBI-881943

Protein

aceF

PMID:15690043

Experiment(s):EBI-881943

Protein

dnaJ

PMID:15690043

Experiment(s):EBI-881943

Protein

lpdA

PMID:15690043

Experiment(s):EBI-881943, EBI-889451

Protein

rnhA

PMID:15690043

Experiment(s):EBI-881943, EBI-881230, EBI-889451

Protein

sbcB

PMID:15690043

Experiment(s):EBI-881943

Protein

topB

PMID:15690043

Experiment(s):EBI-881943

Protein

tufA

PMID:15690043

Experiment(s):EBI-881943

Protein

dnaK

PMID:15690043

Experiment(s):EBI-889451

Protein

galR

PMID:15690043

Experiment(s):EBI-889451

Protein

mtlD

PMID:15690043

Experiment(s):EBI-889451

Protein

rplD

PMID:15690043

Experiment(s):EBI-889451

Protein

rplV

PMID:15690043

Experiment(s):EBI-889451

Protein

rpmB

PMID:15690043

Experiment(s):EBI-889451

Protein

rpsP

PMID:15690043

Experiment(s):EBI-889451

Protein

ruvA

PMID:15690043

Experiment(s):EBI-889451

Protein

yhbY

PMID:15690043

Experiment(s):EBI-889451

Protein

ruvA

PMID:19402753

LCMS(ID Probability):99.6

Protein

mtlD

PMID:19402753

LCMS(ID Probability):99.0

Protein

yhbY

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplD

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpmB

PMID:19402753

LCMS(ID Probability):99.6

Protein

topB

PMID:19402753

MALDI(Z-score):24.545628

Protein

ssb

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):38.080774

Protein

rnhA

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):31.081758

Protein

galR

PMID:19402753

LCMS(ID Probability):99.0

Protein

sbcB

PMID:19402753

MALDI(Z-score):25.609369

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKQQIQLRRR EVDETADLPA ELPPLLRRLY ASRGVRSAQE LERSVKGMLP WQQLSGVEKA
VEILYNAFRE GTRIIVVGDF DADGATSTAL SVLAMRSLGC SNIDYLVPNR FEDGYGLSPE
VVDQAHARGA QLIVTVDNGI SSHAGVEHAR SLGIPVIVTD HHLPGDTLPA AEAIINPNLR
DCNFPSKSLA GVGVAFYLML ALRTFLRDQG WFDERNIAIP NLAELLDLVA LGTVADVVPL
DANNRILTWQ GMSRIRAGKC RPGIKALLEV ANRDAQKLAA SDLGFALGPR LNAAGRLDDM
SVGVALLLCD NIGEARVLAN ELDALNQTRK EIEQGMQIEA LTLCEKLERS RDTLPGGLAM
YHPEWHQGVV GILASRIKER FHRPVIAFAP AGDGTLKGSG RSIQGLHMRD ALERLDTLYP
GMMLKFGGHA MAAGLSLEED KFKLFQQRFG ELVTEWLDPS LLQGEVVSDG PLSPAEMTME
VAQLLRDAGP WGQMFPEPLF DGHFRLLQQR LVGERHLKVM VEPVGGGPLL DGIAFNVDTA
LWPDNGVREV QLAYKLDINE FRGNRSLQII IDNIWPI
Length

577

Mol. Wt

63.39 kDa

pI

5.4 (calculated)

Extinction coefficient

55,920 - 56,545 (calc based on 8 Y, 8 W, and 5 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

382..451

PF02272 DHHA1 domain

PMID:19920124

Domain

67..234

PF01368 DHH family

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=recJ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130794

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947367

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009492

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P21893

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10830

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10830

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947367

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000821

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0823

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

4.998+/-0.084

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.051067513

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

504

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

118

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

259

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

dsbC-recJ-prfB

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3036108..3036148 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2892 (EcoliWiki Page)

NCBI GEO profiles for recJ

microarray

GenExpDB:b2892 (EcoliWiki Page)

Summary of data for recJ from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to recJ Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10830

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0823

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2892

EcoGene

EcoGene:EG10830

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000821

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009492

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Xenopus tropicalis

  • ENSXETP00000028748 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

RECJ

From SHIGELLACYC

E. coli O157

RECJ

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01368 DHH family

Pfam (EcoliWiki Page)

PF02272 DHHA1 domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:64182

EcoCyc

EcoCyc:EG10830

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10830

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000821

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0823

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009492

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 6.2 Lovett, ST & Clark, AJ (1984) Genetic analysis of the recJ gene of Escherichia coli K-12. J. Bacteriol. 157 190-6 PubMed
  7. ↑ Gillen, JR et al. (1981) Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12. J. Bacteriol. 145 521-32 PubMed
  8. ↑ Horii, Z & Clark, AJ (1973) Genetic analysis of the recF pathway to genetic recombination in Escherichia coli K12: isolation and characterization of mutants. J. Mol. Biol. 80 327-44 PubMed
  9. ↑ Haggerty, TJ & Lovett, ST (1997) IF3-mediated suppression of a GUA initiation codon mutation in the recJ gene of Escherichia coli. J. Bacteriol. 179 6705-13 PubMed
  10. ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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