recJ:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RecJ

Synonyms

ssDNA exonuclease, 5' --> 3'-specific[1], B2892[2][1], RecJ[2][1] , ECK2887, JW2860, b2892

Product description

RecJ[2][3]

ssDNA 5'-3' exonuclease involved in recombination[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003156

F

Seeded from EcoCyc (v14.0)

complete

GO:0004518

nuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0540

F

Seeded from EcoCyc (v14.0)

complete

GO:0004527

exonuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0269

F

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004610

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004610

P

Seeded from EcoCyc (v14.0)

complete

GO:0008409

5'-3' exonuclease activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004610

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001667

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0030145

manganese ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001667

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

flhA

PMID:16606699[5]

Experiment(s):EBI-1144203

Protein

aceE

PMID:15690043[6]

Experiment(s):EBI-881943

Protein

aceF

PMID:15690043[6]

Experiment(s):EBI-881943

Protein

dnaJ

PMID:15690043[6]

Experiment(s):EBI-881943

Protein

lpdA

PMID:15690043[6]

Experiment(s):EBI-881943, EBI-889451

Protein

rnhA

PMID:15690043[6]

Experiment(s):EBI-881943, EBI-881230, EBI-889451

Protein

sbcB

PMID:15690043[6]

Experiment(s):EBI-881943

Protein

topB

PMID:15690043[6]

Experiment(s):EBI-881943

Protein

tufA

PMID:15690043[6]

Experiment(s):EBI-881943

Protein

dnaK

PMID:15690043[6]

Experiment(s):EBI-889451

Protein

galR

PMID:15690043[6]

Experiment(s):EBI-889451

Protein

mtlD

PMID:15690043[6]

Experiment(s):EBI-889451

Protein

rplD

PMID:15690043[6]

Experiment(s):EBI-889451

Protein

rplV

PMID:15690043[6]

Experiment(s):EBI-889451

Protein

rpmB

PMID:15690043[6]

Experiment(s):EBI-889451

Protein

rpsP

PMID:15690043[6]

Experiment(s):EBI-889451

Protein

ruvA

PMID:15690043[6]

Experiment(s):EBI-889451

Protein

yhbY

PMID:15690043[6]

Experiment(s):EBI-889451

Protein

ruvA

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

mtlD

PMID:19402753[7]

LCMS(ID Probability):99.0

Protein

yhbY

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplD

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rpmB

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

topB

PMID:19402753[7]

MALDI(Z-score):24.545628

Protein

ssb

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):38.080774

Protein

rnhA

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):31.081758

Protein

galR

PMID:19402753[7]

LCMS(ID Probability):99.0

Protein

sbcB

PMID:19402753[7]

MALDI(Z-score):25.609369

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKQQIQLRRR EVDETADLPA ELPPLLRRLY ASRGVRSAQE LERSVKGMLP WQQLSGVEKA
VEILYNAFRE GTRIIVVGDF DADGATSTAL SVLAMRSLGC SNIDYLVPNR FEDGYGLSPE
VVDQAHARGA QLIVTVDNGI SSHAGVEHAR SLGIPVIVTD HHLPGDTLPA AEAIINPNLR
DCNFPSKSLA GVGVAFYLML ALRTFLRDQG WFDERNIAIP NLAELLDLVA LGTVADVVPL
DANNRILTWQ GMSRIRAGKC RPGIKALLEV ANRDAQKLAA SDLGFALGPR LNAAGRLDDM
SVGVALLLCD NIGEARVLAN ELDALNQTRK EIEQGMQIEA LTLCEKLERS RDTLPGGLAM
YHPEWHQGVV GILASRIKER FHRPVIAFAP AGDGTLKGSG RSIQGLHMRD ALERLDTLYP
GMMLKFGGHA MAAGLSLEED KFKLFQQRFG ELVTEWLDPS LLQGEVVSDG PLSPAEMTME
VAQLLRDAGP WGQMFPEPLF DGHFRLLQQR LVGERHLKVM VEPVGGGPLL DGIAFNVDTA
LWPDNGVREV QLAYKLDINE FRGNRSLQII IDNIWPI
Length

577

Mol. Wt

63.39 kDa

pI

5.4 (calculated)

Extinction coefficient

55,920 - 56,545 (calc based on 8 Y, 8 W, and 5 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

382..451

PF02272 DHHA1 domain

PMID:19920124[8]

Domain

67..234

PF01368 DHH family

PMID:19920124[8]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=recJ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130794

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947367

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009492

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P21893

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10830

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10830

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947367

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000821

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0823

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 6.14 6.15 6.16 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 7.8 7.9 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  8. ↑ 8.0 8.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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