recG:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

recG

Gene Synonym(s)

ECK3642, b3652, JW3627, spoV, radC[1], radC

Product Desc.

RecG, DNA helicase, resolution of Holliday junctions, branch migration[2][3]

Junction-specific ATP-dependent DNA helicase; facilitates branch migration of Holliday junctions; allelic to radC102; overexpression improves resistance to weak organic acids[4]

Product Synonyms(s)

ATP-dependent DNA helicase[1], B3652[2][1], SpoV[2][1], RadC[2][1], RecG[2][1] , ECK3642, JW3627, radC, spoV, b3652

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): rpoZ-spoT-trmH-recG[2], rpoZ-spoTU-recG

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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See the ruvABC entries for additional information. otherPDB (T. maritima): 1GM5.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

recG

Mnemonic

Recombination

Synonyms

ECK3642, b3652, JW3627, spoV, radC[1], radC

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

82.4 minutes 

MG1655: 3823233..3825314
<gbrowseImage> name=NC_000913:3823233..3825314 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3763567..3765648
<gbrowseImage> name=NC_012967:3763567..3765648 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3711566..3713647
<gbrowseImage> name=NC_012759:3711566..3713647 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3815205..3813124
<gbrowseImage> name=NC_007779:3813124..3815205 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3920817..3922898
<gbrowseImage> name=NC_010473:3920817..3922898 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrecG (Keio:JW3627)

deletion

deletion

PMID:16738554

Shigen
CGSC10665[5]

recG::Tn5KAN-I-SceI (FB21370)

Insertion at nt 155 in Plus orientation

PMID:15262929

E. coli Genome Project:FB21370

contains pKD46

recG::Tn5KAN-I-SceI (FB21371)

Insertion at nt 155 in Plus orientation

PMID:15262929

E. coli Genome Project:FB21371

does not contain pKD46

recG

deletion

Sensitivity to

decreases sensitivity to Bicyclomycin

PMID:21357484

fig 2

recG2102

CGSC:13916

recG162

CGSC:26965

recG263(::kan)

PMID:8648624

CGSC:69150

recG265(::cat)

CGSC:69152

ΔrecG756::kan

PMID:16738554

CGSC:102626


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3627

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAAGGTCGCCTGTTAGATGC

Primer 2:CCgGCATTCGAGTAACGTTCCGT

7F3

Kohara Phage

Genobase

PMID:3038334

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[5]

est. P1 cotransduction: % [6]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[5]

est. P1 cotransduction: 86% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10829

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10829

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000820

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948162

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0822

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011939

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RecG

Synonyms

ATP-dependent DNA helicase[1], B3652[2][1], SpoV[2][1], RadC[2][1], RecG[2][1] , ECK3642, JW3627, radC, spoV, b3652

Product description

RecG, DNA helicase, resolution of Holliday junctions, branch migration[2][3]

Junction-specific ATP-dependent DNA helicase; facilitates branch migration of Holliday junctions; allelic to radC102; overexpression improves resistance to weak organic acids[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001650

F

Seeded from EcoCyc (v14.0)

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004365

F

Seeded from EcoCyc (v14.0)

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011545

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0004003

ATP-dependent DNA helicase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004609

F

Seeded from EcoCyc (v14.0)

complete

GO:0004386

helicase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001650

F

Seeded from EcoCyc (v14.0)

complete

GO:0004386

helicase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0347

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001650

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011545

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004609

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0234

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004609

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0233

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

Seeded from EcoCyc (v14.0)

complete

GO:0008026

ATP-dependent helicase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011545

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

aceF

PMID:15690043

Experiment(s):EBI-882119

Protein

rrmA

PMID:16606699

Experiment(s):EBI-1146417

Protein

rpsC

PMID:16606699

Experiment(s):EBI-1146417

Protein

yjbD

PMID:16606699

Experiment(s):EBI-1146417

Protein

rplB

PMID:16606699

Experiment(s):EBI-1146417

Protein

relE

PMID:16606699

Experiment(s):EBI-1146417

Protein

rplQ

PMID:16606699

Experiment(s):EBI-1146417

Protein

rpsG

PMID:16606699

Experiment(s):EBI-1146417

Protein

rpsM

PMID:16606699

Experiment(s):EBI-1146417

Protein

fkpA

PMID:16606699

Experiment(s):EBI-1146417

Protein

yfiE

PMID:16606699

Experiment(s):EBI-1146417

Protein

rplA

PMID:16606699

Experiment(s):EBI-1146417

Protein

cybC

PMID:16606699

Experiment(s):EBI-1146417

Protein

nadE

PMID:16606699

Experiment(s):EBI-1146417

Protein

ssb

PMID:16606699

Experiment(s):EBI-1146417

Protein

diaA

PMID:16606699

Experiment(s):EBI-1146417

Protein

rpsI

PMID:16606699

Experiment(s):EBI-1146417

Protein

rplD

PMID:16606699

Experiment(s):EBI-1146417

Protein

rpsD

PMID:16606699

Experiment(s):EBI-1146417

Protein

yghW

PMID:16606699

Experiment(s):EBI-1146417
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKGRLLDAVP LSSLTGVGAA LSNKLAKINL HTVQDLLLHL PLRYEDRTHL YPIGELLPGV
YATVEGEVLN CNISFGGRRM MTCQISDGSG ILTMRFFNFS AAMKNSLAAG RRVLAYGEAK
RGKYGAEMIH PEYRVQGDLS TPELQETLTP VYPTTEGVKQ ATLRKLTDQA LDLLDTCAIE
ELLPPELSQG MMTLPEALRT LHRPPPTLQL SDLETGQHPA QRRLILEELL AHNLSMLALR
AGAQRFHAQP LSANDTLKNK LLAALPFKPT GAQARVVAEI ERDMALDVPM MRLVQGDVGS
GKTLVAALAA LRAIAHGKQV ALMAPTELLA EQHANNFRNW FAPLGIEVGW LAGKQKGKAR
LAQQEAIASG QVQMIVGTHA IFQEQVQFNG LALVIIDEQH RFGVHQRLAL WEKGQQQGFH
PHQLIMTATP IPRTLAMTAY ADLDTSVIDE LPPGRTPVTT VAIPDTRRTD IIDRVHHACI
TEGRQAYWVC TLIEESELLE AQAAEATWEE LKLALPELNV GLVHGRMKPA EKQAVMASFK
QGELHLLVAT TVIEVGVDVP NASLMIIENP ERLGLAQLHQ LRGRVGRGAV ASHCVLLYKT
PLSKTAQIRL QVLRDSNDGF VIAQKDLEIR GPGELLGTRQ TGNAEFKVAD LLRDQAMIPE
VQRLARHIHE RYPQQAKALI ERWMPETERY SNA
Length

693

Mol. Wt

76.431 kDa

pI

7.3 (calculated)

Extinction coefficient

50,880 - 51,630 (calc based on 12 Y, 6 W, and 6 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

270..438

PF00270 DEAD/DEAH box helicase

PMID:19920124

Domain

511..589

PF00271 Helicase conserved C-terminal domain

PMID:19920124

Domain

62..136

PF01336 OB-fold nucleic acid binding domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=recG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131523

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948162

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011939

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P24230

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10829

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10829

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948162

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000820

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0822

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

6.894+/-0.041

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.02666987

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

112

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

56

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

85

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

rpoZ-spoT-trmH-recG

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3823213..3823253 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3652 (EcoliWiki Page)

NCBI GEO profiles for recG

microarray

GenExpDB:b3652 (EcoliWiki Page)

Summary of data for recG from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to recG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10829

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0822

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3652

EcoGene

EcoGene:EG10829

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000820

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011939

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000028393 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT2G01440 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q6ZFX9 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

RECG

From SHIGELLACYC

E. coli O157

RECG

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01336 OB-fold nucleic acid binding domain

Pfam (EcoliWiki Page)

PF00271 Helicase conserved C-terminal domain

Pfam (EcoliWiki Page)

PF00270 DEAD/DEAH box helicase

Superfamily (EcoliWiki Page)

SUPERFAMILY:50249

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

Panther (EcoliWiki Page)

PTHR24031:SF174

EcoCyc

EcoCyc:EG10829

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10829

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000820

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0822

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011939

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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