recG:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

recG

Mnemonic

Recombination

Synonyms

ECK3642, b3652, JW3627, spoV, radC[1], radC

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

82.4 minutes 

MG1655: 3823233..3825314
<gbrowseImage> name=NC_000913:3823233..3825314 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3763567..3765648
<gbrowseImage> name=NC_012967:3763567..3765648 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3711566..3713647
<gbrowseImage> name=NC_012759:3711566..3713647 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3815205..3813124
<gbrowseImage> name=NC_007779:3813124..3815205 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3920817..3922898
<gbrowseImage> name=NC_010473:3920817..3922898 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrecG (Keio:JW3627)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10665[3]

recG::Tn5KAN-I-SceI (FB21370)

Insertion at nt 155 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB21370

contains pKD46

recG::Tn5KAN-I-SceI (FB21371)

Insertion at nt 155 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB21371

does not contain pKD46

recG

deletion

Sensitivity to

decreases sensitivity to Bicyclomycin

PMID:21357484[5]

fig 2

recG2102

CGSC:13916

recG162

CGSC:26965

recG263(::kan)

PMID:8648624[6]

CGSC:69150

recG265(::cat)

CGSC:69152

ΔrecG756::kan

PMID:16738554[2]

CGSC:102626


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3627

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAAAGGTCGCCTGTTAGATGC

Primer 2:CCgGCATTCGAGTAACGTTCCGT

7F3

Kohara Phage

Genobase

PMID:3038334[8]

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[3]

est. P1 cotransduction: % [9]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[3]

est. P1 cotransduction: 86% [9]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10829

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10829

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000820

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948162

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0822

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011939

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
  6. Mahdi, AA et al. (1996) Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82. J. Mol. Biol. 257 561-73 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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