{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	recE
Gene Synonym(s)	ECK1347, b1350, JW1344, rac, sbcA, rmuB^[1], rmuB
Product Desc.	exonuclease VIII, ds DNA exonuclease, 5' --> 3' specific^[2]^[3] RecET recombinase, exonuclease VIII, Rac prophage; recombination and repair; degrades one strand 5'-3' in duplex DNA^[4]
Product Synonyms(s)	Rac prophage; exonuclease VIII, 5' -> 3' specific dsDNA exonuclease^[1], B1350^[2]^[1], RmuB^[2]^[1], Rac^[2]^[1], SbcA^[2]^[1], RecE^[2]^[1] , ECK1347, JW1344, rmuB, sbcA, b1350
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): intR^[2]
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

RecET promotes homologous recombinarion between short regions of homology, analagous to the lambda Red recombinase. sbcA suppressor mutations allow expression of recE, in some cases by fusing to the upstream racC gene.^[4]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	recE
Mnemonic	Recombination
Synonyms	ECK1347, b1350, JW1344, rac, sbcA, rmuB^[1], rmuB
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	30.45 minutes	MG1655: 1415410..1412810 <gbrowseImage> name=NC_000913:1412810..1415410 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1414556..1411956 <gbrowseImage> name=NC_012967:1411956..1414556 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1304869..1307469 <gbrowseImage> name=NC_012759:1304869..1307469 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1419100..1416500 <gbrowseImage> name=NC_007779:1416500..1419100 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1504806..1502206 <gbrowseImage> name=NC_010473:1502206..1504806 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1412813	Edman degradation	PMID:3056915
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔrecE (Keio:JW1344)	deletion	deletion	PMID:16738554	Shigen CGSC9198^[5]
recE101::Tn10				CGSC:84457
ΔrecE787::kan			PMID:16738554	CGSC:102624
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1344	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGCACAAAACCACTCTTCCT Primer 2:CCGTCATTTGCATATTCCTTAGC
4A6	Kohara Phage	Genobase	PMID:3038334
2C3	Kohara Phage	Genobase	PMID:3038334
recT3061::Tn10	Linked marker	CAG12081 = CGSC7375^[5]	est. P1 cotransduction: 93% ^[6] Synonyms:zcj-3061::Tn10, zda-3061::Tn10
zhj-3076::Tn10	Linked marker	CAG18640 = CGSC7453^[5]	est. P1 cotransduction: 47% ^[6]
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10827	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10827	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000818	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945918	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0820	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004531	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	RecE
Synonyms	Rac prophage; exonuclease VIII, 5' -> 3' specific dsDNA exonuclease^[1], B1350^[2]^[1], RmuB^[2]^[1], Rac^[2]^[1], SbcA^[2]^[1], RecE^[2]^[1] , ECK1347, JW1344, rmuB, sbcA, b1350
Product description	exonuclease VIII, ds DNA exonuclease, 5' --> 3' specific^[2]^[3] RecET recombinase, exonuclease VIII, Rac prophage; recombination and repair; degrades one strand 5'-3' in duplex DNA^[4]
EC number (for enzymes)	3.1.11.-^[1]
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004518	nuclease activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0540	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004527	exonuclease activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0269	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	aceE	PMID:15690043	Experiment(s):EBI-882168
Protein	ligA	PMID:15690043	Experiment(s):EBI-882168, EBI-883824
Protein	rpsB	PMID:16606699	Experiment(s):EBI-1139608
Protein	mreB	PMID:16606699	Experiment(s):EBI-1139608
Protein	recG	PMID:16606699	Experiment(s):EBI-1139608
Protein	rfaG	PMID:16606699	Experiment(s):EBI-1139608
Protein	glpR	PMID:16606699	Experiment(s):EBI-1139608
Protein	pheA	PMID:16606699	Experiment(s):EBI-1139608
Protein	idnO	PMID:16606699	Experiment(s):EBI-1139608
Protein	ligA	PMID:19402753	MALDI(Z-score):23.878210
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSTKPLFLLR KAKKSSGEPD VVLWASNDFE STCATLDYLI VKSGKKLSSY FKAVATNFPV VNDLPAEGEI DFTWSERYQL SKDSMTWELK PGAAPDNAHY QGNTNVNGED MTEIEENMLL PISGQELPIR WLAQHGSEKP VTHVSRDGLQ ALHIARAEEL PAVTALAVSH KTSLLDPLEI RELHKLVRDT DKVFPNPGNS NLGLITAFFE AYLNADYTDR GLLTKEWMKG NRVSHITRTA SGANAGGGNL TDRGEGFVHD LTSLARDVAT GVLARSMDLD IYNLHPAHAK RIEEIIAENK PPFSVFRDKF ITMPGGLDYS RAIVVASVKE APIGIEVIPA HVTEYLNKVL TETDHANPDP EIVDIACGRS SAPMPQRVTE EGKQDDEEKP QPSGTTAVEQ GEAETMEPDA TEHHQDTQPL DAQSQVNSVD AKYQELRAEL HEARKNIPSK NPVDDDKLLA ASRGEFVDGI SDPNDPKWVK GIQTRDCVYQ NQPETEKTSP DMNQPEPVVQ QEPEIACNAC GQTGGDNCPD CGAVMGDATY QETFDEESQV EAKENDPEEM EGAEHPHNEN AGSDPHRDCS DETGEVADPV IVEDIEPGIY YGISNENYHA GPGISKSQLD DIADTPALYL WRKNAPVDTT KTKTLDLGTA FHCRVLEPEE FSNRFIVAPE FNRRTNAGKE EEKAFLMECA STGKTVITAE EGRKIELMYQ SVMALPLGQW LVESAGHAES SIYWEDPETG ILCRCRPDKI IPEFHWIMDV KTTADIQRFK TAYYDYRYHV QDAFYSDGYE AQFGVQPTFV FLVASTTIEC GRYPVEIFMM GEEAKLAGQQ EYHRNLRTLS DCLNTDEWPA IKTLSLPRWA KEYAND
Length	866
Mol. Wt	96.368 kDa
pI	4.6 (calculated)
Extinction coefficient	106,230 - 107,980 (calc based on 27 Y, 12 W, and 14 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..178	PF06630 Enterobacterial exodeoxyribonuclease VIII		PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=recE taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129311	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945918	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004531	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P15032	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10827	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10827	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945918	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000818	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0820	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	0.183+/-0.027	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.0025	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	15	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	8	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
intR
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:1415390..1415430 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1350 (EcoliWiki Page)	NCBI GEO profiles for recE
microarray	GenExpDB:b1350 (EcoliWiki Page)	Summary of data for recE from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1415323..1415567) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ04; Well:D9^[7]
edit table

Notes

Accessions Related to recE Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10827	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0820	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1350
EcoGene	EcoGene:EG10827	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000818	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004531	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	S1955	From SHIGELLACYC
E. coli O157	RECE	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF06630 Enterobacterial exodeoxyribonuclease VIII
EcoCyc	EcoCyc:EG10827	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10827	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000818	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0820	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004531	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

recE:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to recE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search