rcsC:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
rcsC |
---|---|
Gene Synonym(s) |
ECK2211, b2218, JW5917, JW5920, JW5920+JW5917[1] |
Product Desc. |
Negative regulatory gene for capsule (colanic acid) synthesis, controls sliminess; contains TerF; probable histidine kinase[4] |
Product Synonyms(s) |
hybrid sensory kinase in two-component regulatory system with RcsB and YojN[1], B2218[2][1], sensor protein RcsC-Phis[2][1], ECK2211, JW5917, JW5920, b2218 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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Notes
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
rcsC |
---|---|
Mnemonic |
Regulator of capsule synthesis |
Synonyms |
ECK2211, b2218, JW5917, JW5920, JW5920+JW5917[1] |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
49.9 minutes, 49.9 minutes |
MG1655: 2317850..2315049 |
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NC_012967: 2266528..2263727 |
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NC_012759: 2200854..2203703 |
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W3110 |
|
W3110: 2320735..2320361 |
||
W3110 |
|
W3110: 2324498..2322075 |
||
W3110 |
|
W3110: 2324498..2320361 |
||
DH10B: 2408886..2406037 |
||||
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
ΔrcsC (Keio:JW5917) |
deletion |
deletion |
PMID:16738554 |
||||
rcsC::Tn5KAN-2 (FB20714) |
Insertion at nt 1312 in Minus orientation |
PMID:15262929 |
contains pKD46 | ||||
rcsC::Tn5KAN-2 (FB20715) |
Insertion at nt 1312 in Minus orientation |
PMID:15262929 |
does not contain pKD46 | ||||
rcsCD948E |
D948E |
(in strain: O9:K30:H12) |
Strain variation; seeded from UniProt:P14376 | ||||
rcsCL315V |
L315V |
(in strain: O9:K30:H12) |
Strain variation; seeded from UniProt:P14376 | ||||
rcsCK776E |
K776E |
(in strain: O9:K30:H12) |
Strain variation; seeded from UniProt:P14376 | ||||
rcsCI890T |
I890T |
(in strain: O9:K30:H12) |
Strain variation; seeded from UniProt:P14376 | ||||
rcsCL938V |
L938V |
(in strain: O9:K30:H12) |
Strain variation; seeded from UniProt:P14376 | ||||
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
Plasmid clone |
PMID:16769691 Status: Primer 1: Primer 2: | ||
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 62% [6] | ||
Linked marker |
est. P1 cotransduction: 85% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10822 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10822 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000813 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB0815 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0007336 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
RcsC |
---|---|
Synonyms |
hybrid sensory kinase in two-component regulatory system with RcsB and YojN[1], B2218[2][1], sensor protein RcsC-Phis[2][1], ECK2211, JW5917, JW5920, b2218 |
Product description |
Negative regulatory gene for capsule (colanic acid) synthesis, controls sliminess; contains TerF; probable histidine kinase[4] |
EC number (for enzymes) |
|
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0000155 |
two-component sensor activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003661 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000155 |
two-component sensor activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005467 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000156 |
two-component response regulator activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001789 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001789 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019017 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0902 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0547 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004673 |
protein histidine kinase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005467 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004673 |
protein histidine kinase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019017 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004673 |
protein histidine kinase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
EC:2.7.13.3 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004871 |
signal transducer activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000014 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004871 |
signal transducer activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR009082 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004871 |
signal transducer activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019017 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003594 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019017 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0067 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005886 |
plasma membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019017 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0997 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-1003 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006355 |
regulation of transcription, DNA-dependent |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001789 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006355 |
regulation of transcription, DNA-dependent |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019017 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0007165 |
signal transduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000014 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0007165 |
signal transduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003661 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0007165 |
signal transduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005467 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0007165 |
signal transduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR009082 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016020 |
membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003661 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016020 |
membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0472 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019017 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016021 |
integral to membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0812 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016021 |
integral to membrane |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-9909 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016301 |
kinase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0418 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016310 |
phosphorylation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004358 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016740 |
transferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0808 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016772 |
transferase activity, transferring phosphorus-containing groups |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004358 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0018106 |
peptidyl-histidine phosphorylation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005467 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0018106 |
peptidyl-histidine phosphorylation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019017 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions
RcsC-Phis
See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
plasma membrane |
From EcoCyc[3] |
| ||
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MFRALALVLW LLIAFSSVFY IVNALHQRES EIRQEFNLSS DQAQRFIQRT SDVMKELKYI AENRLSAENG VLSPRGRETQ ADVPAFEPLF ADSDCSAMSN TWRGSLESLA WFMRYWRDNF SAAYDLNRVF LIGSDNLCMA NFGLRDMPVE RDTALKALHE RINKYRNAPQ DDSGSNLYWI SEGPRPGVGY FYALTPVYLA NRLQALLGVE QTIRMENFFL PGTLPMGVTI LDENGHTLIS LTGPESKIKG DPRWMQERSW FGYTEGFREL VLKKNLPPSS LSIVYSVPVD KVLERIRMLI LNAILLNVLA GAALFTLARM YERRIFIPAE SDALRLEEHE QFNRKIVASA PVGICILRTA DGVNILSNEL AHTYLNMLTH EDRQRLTQII CGQQVNFVDV LTSNNTNLQI SFVHSRYRNE NVAICVLVDV SSRVKMEESL QEMAQAAEQA SQSKSMFLAT VSHELRTPLY GIIGNLDLLQ TKELPKGVDR LVTAMNNSSS LLLKIISDIL DFSKIESEQL KIEPREFSPR EVMNHITANY LPLVVRKQLG LYCFIEPDVP VALNGDPMRL QQVISNLLSN AIKFTDTGCI VLHVRADGDY LSIRVRDTGV GIPAKEVVRL FDPFFQVGTG VQRNFQGTGL GLAICEKLIS MMDGDISVDS EPGMGSQFTV RIPLYGAQYP QKKGVEGLSG KRCWLAVRNA SLCQFLETSL QRSGIVVTTY EGQEPTPEDV LITDEVVSKK WQGRAVVTFC RRHIGIPLEK APGEWVHSVA APHELPALLA RIYLIEMESD DPANALPSTD KAVSDNDDMM ILVVDDHPIN RRLLADQLGS LGYQCKTAND GVDALNVLSK NHIDIVLSDV NMPNMDGYRL TQRIRQLGLT LPVIGVTANA LAEEKQRCLE SGMDSCLSKP VTLDVIKQTL TLYAERVRKS RDS |
Length |
933 |
Mol. Wt |
104.59 kDa |
pI |
5.9 (calculated) |
Extinction coefficient |
92,250 - 94,000 (calc based on 25 Y, 10 W, and 14 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0007336 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG10822 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10822 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120000813 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0815 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
Ecoli K-12 |
8.874+/-0.205 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.030748663 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
Protein |
E. coli K-12 MG1655 |
102 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
70 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
60 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:2317830..2317870
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for rcsC | |
microarray |
Summary of data for rcsC from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
GFP Fusion |
Intergenic region (2317751..2318083) fused to gfpmut2. |
GFP fusion described in Zaslaver, et al. | ||
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Notes
Accessions Related to rcsC Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10822 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0815 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10822 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000813 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0007336 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Apis mellifera |
|
From Inparanoid:20070104 |
Arabidopsis thaliana |
|
From Inparanoid:20070104 |
Oryza gramene |
|
From Inparanoid:20070104 |
Saccharomyces cerevisiae |
|
From Inparanoid:20070104 |
Xenopus tropicalis |
|
From Inparanoid:20070104 |
Shigella flexneri |
RCSC |
From SHIGELLACYC |
E. coli O157 |
RCSC |
From ECOO157CYC |
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Notes
Families
<protect>
See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.
Database | Accession | Notes |
---|---|---|
PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed
Categories
- TMHMM Prediction
- Genes in OpenBioSystems with Promoter Fusions
- Genes with homologs in Apis mellifera
- Genes with homologs in Arabidopsis thaliana
- Genes with homologs in Oryza gramene
- Genes with homologs in Saccharomyces cerevisiae
- Genes with homologs in Xenopus tropicalis
- Genes with homologs in Shigella flexneri
- Genes with homologs in E. coli O157