rcsC:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rcsC

Mnemonic

Regulator of capsule synthesis

Synonyms

ECK2211, b2218, JW5917, JW5920, JW5920+JW5917[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

49.9 minutes, 49.9 minutes 

MG1655: 2317850..2315049
<gbrowseImage> name=NC_000913:2315049..2317850 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2266528..2263727
<gbrowseImage> name=NC_012967:2263727..2266528 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2200854..2203703
<gbrowseImage> name=NC_012759:2200854..2203703 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2320735..2320361
<gbrowseImage> name=NC_007779:2320361..2320735 source=W3110 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2324498..2322075
<gbrowseImage> name=NC_007779:2322075..2324498 source=W3110 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2324498..2320361
<gbrowseImage> name=NC_007779:2320361..2324498 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2408886..2406037
<gbrowseImage> name=NC_010473:2406037..2408886 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrcsC (Keio:JW5917)

deletion

deletion

PMID:16738554[2]

Shigen

rcsC::Tn5KAN-2 (FB20714)

Insertion at nt 1312 in Minus orientation

PMID:15262929[3]

E. coli Genome Project:FB20714

contains pKD46

rcsC::Tn5KAN-2 (FB20715)

Insertion at nt 1312 in Minus orientation

PMID:15262929[3]

E. coli Genome Project:FB20715

does not contain pKD46

rcsCD948E

D948E

(in strain: O9:K30:H12)

Strain variation; seeded from UniProt:P14376

rcsCL315V

L315V

(in strain: O9:K30:H12)

Strain variation; seeded from UniProt:P14376

rcsCK776E

K776E

(in strain: O9:K30:H12)

Strain variation; seeded from UniProt:P14376

rcsCI890T

I890T

(in strain: O9:K30:H12)

Strain variation; seeded from UniProt:P14376

rcsCL938V

L938V

(in strain: O9:K30:H12)

Strain variation; seeded from UniProt:P14376

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[4]

Status:

Primer 1:

Primer 2:

22A6

Kohara Phage

Genobase

PMID:3038334[5]

5A1

Kohara Phage

Genobase

PMID:3038334[5]

4F12

Kohara Phage

Genobase

PMID:3038334[5]

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[6]

est. P1 cotransduction: 62% [7]
Synonyms:zeg-722, zei-722::Tn10

atoS298::Tn10

Linked marker

CAG12177 gyrA+ = CGSC7525[6]

est. P1 cotransduction: 85% [7]
Synonyms:zeh-298::Tn10, zej-298::Tn10 nnnThe original CAG12177 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10822

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10822

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000813

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948993

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0815

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007336

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 5.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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