putP:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
putP |
|---|---|
| Mnemonic |
Proline utilization |
| Synonyms |
ECK1006, b1015, JW1001, putC[1], putC |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
23.25 minutes |
MG1655: 1078528..1080036 |
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NC_012967: 1095589..1097097 |
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NC_012759: 981496..983004 |
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W3110 |
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W3110: 1079727..1081235 |
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DH10B: 1132456..1133964 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
1078528 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔputP (Keio:JW1001) |
deletion |
deletion |
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putPR257C |
R257C |
Sodium-independent binding affinity for proline |
seeded from UniProt:P07117 | ||||
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putPR376E,Q |
R376E,Q |
No change in activity |
seeded from UniProt:P07117 | ||||
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putPR376K |
R376K |
Loss of activity |
seeded from UniProt:P07117 | ||||
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putP5::Tn5 |
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ΔputP760::kan |
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putAP(del)101 |
Auxotrophies |
Proline Auxotroph |
Strains: RM2 |
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|
putP putA + |
Resistant to |
Dehydroproline resistance |
Strain:CSH4 |
table 2 | |||
|
putP putA + |
Resistant to |
L-Azetidine-2-carboxylate resistance |
Strain:CSH4 |
table 2 | |||
|
putP in strain C4 |
Resistant to |
L-Azetidine-2-carboxylate resistance |
Strain: C4 |
Table 1 | |||
|
putP in strain C4 |
Resistant to |
Dehydroproline resistance |
Strain: C4 |
Table 1 | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1001 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGCTATTAGCACACCGATGTT Primer 2:CCGCTTTCCTGCAACCGTGACGG | |
|
Kohara Phage |
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|
Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 57% [10] | ||
|
Linked marker |
est. P1 cotransduction: 1% [10] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Hanada, K et al. (1992) Sodium ion and proline binding sites in the Na+/proline symport carrier of Escherichia coli. Biochim. Biophys. Acta 1105 61-6 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ Stalmach, ME et al. (1983) Two proline porters in Escherichia coli K-12. J. Bacteriol. 156 481-6 PubMed
- ↑ 6.0 6.1 Wood, JM (1981) Genetics of L-proline utilization in Escherichia coli. J. Bacteriol. 146 895-901 PubMed
- ↑ 7.0 7.1 Tristram, H & Neale, S (1968) The activity and specificity of the proline permease in wild-type and analogue-resistant strains of Escherichia coli. J. Gen. Microbiol. 50 121-37 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
