PMID:6355059
| Citation |
Stalmach, ME, Grothe, S and Wood, JM (1983) Two proline porters in Escherichia coli K-12. J. Bacteriol. 156:481-6 |
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| Abstract |
Escherichia coli mutants defective at putP and putA lack proline transport via proline porter I and proline dehydrogenase activity, respectively. They retain a proline uptake system (proline porter II) that is induced during tryptophan-limited growth and are sensitive to the toxic L-proline analog, 3,4-dehydroproline. 3,4-Dehydroproline-resistant mutants derived from a putP putA mutant lack proline porter II. Auxotrophic derivatives derived from putP+ or putP bacteria can grow if provided with proline at low concentration (25 microM); those derived from the 3,4-dehydroproline-resistant mutants require high proline for growth (2.5 mM). We conclude that E. coli, like Salmonella typhimurium, possesses a second proline porter that is inactivated by mutations at the proP locus. |
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| Keywords |
Amino Acid Transport Systems, Neutral; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Genotype; Isoenzymes/genetics; Kinetics; Membrane Transport Proteins/genetics; Mutation; Proline/metabolism; Species Specificity |
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