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Standard Name	purU
Gene Synonym(s)	ECK1227, b1232, JW1220, tgs, ychI^[1], ychI
Product Desc.	formyltetrahydrofolate deformylase^[2]^[3]; Component of formyltetrahydrofolate deformylase^[2]^[3] Formyltetrahydrofolate hydrolase; mutation causes transient glycine starvation^[4]
Product Synonyms(s)	formyltetrahydrofolate hydrolase^[1], B1232^[2]^[1], YchI^[2]^[1], Tgs^[2]^[1], PurU^[2]^[1] , ECK1227, JW1220, tgs, ychI, b1232
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): purU^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	purU
Mnemonic	Purine
Synonyms	ECK1227, b1232, JW1220, tgs, ychI^[1], ychI
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	27.74 minutes	MG1655: 1287847..1287005 <gbrowseImage> name=NC_000913:1287005..1287847 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1288418..1287576 <gbrowseImage> name=NC_012967:1287576..1288418 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1175726..1176568 <gbrowseImage> name=NC_012759:1175726..1176568 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1290201..1289359 <gbrowseImage> name=NC_007779:1289359..1290201 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1328088..1327246 <gbrowseImage> name=NC_010473:1327246..1328088 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1287005	Edman degradation	PMID:8226647
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔpurU (Keio:JW1220)	deletion	deletion	PMID:16738554	Shigen CGSC9106^[5]
ΔpurU741::kan			PMID:16738554	CGSC:102548
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1220	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCATTCACTCCAACGTAAAGT Primer 2:CCAAGAATAATCGTTCGATTACC
12H9	Kohara Phage	Genobase	PMID:3038334
4D8	Kohara Phage	Genobase	PMID:3038334
fadR13::Tn10	Linked marker	CAG18497 = CGSC7365^[5]	est. P1 cotransduction: 8% ^[6]
oppC506::Tn10	Linked marker	CAG12169 = CGSC7369^[5]	est. P1 cotransduction: 55% ^[6] Synonyms:zch-506::Tn10, zci-506::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11819	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11819	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001751	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945827	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1766	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004138	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PurU
Synonyms	formyltetrahydrofolate hydrolase^[1], B1232^[2]^[1], YchI^[2]^[1], Tgs^[2]^[1], PurU^[2]^[1] , ECK1227, JW1220, tgs, ychI, b1232
Product description	formyltetrahydrofolate deformylase^[2]^[3]; Component of formyltetrahydrofolate deformylase^[2]^[3] Formyltetrahydrofolate hydrolase; mutation causes transient glycine starvation^[4]
EC number (for enzymes)	3.5.1.10^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006164	purine nucleotide biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0658	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006189	'de novo' IMP biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004810	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006730	one-carbon metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0554	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002912	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008864	formyltetrahydrofolate deformylase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004810	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008864	formyltetrahydrofolate deformylase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.5.1.10	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009058	biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002376	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016597	amino acid binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002912	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016742	hydroxymethyl-, formyl- and related transferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002376	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of formyltetrahydrofolate deformylase	could be indirect
Protein	groL		PMID:16606699	Experiment(s):EBI-1139232
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MHSLQRKVLR TICPDQKGLI ARITNICYKH ELNIVQNNEF VDHRTGRFFM RTELEGIFND STLLADLDSA LPEGSVRELN PAGRRRIVIL VTKEAHCLGD LLMKANYGGL DVEIAAVIGN HDTLRSLVER FDIPFELVSH EGLTRNEHDQ KMADAIDAYQ PDYVVLAKYM RVLTPEFVAR FPNKIINIHH SFLPAFIGAR PYHQAYERGV KIIGATAHYV NDNLDEGPII MQDVIHVDHT YTAEDMMRAG RDVEKNVLSR ALYKVLAQRV FVYGNRTIIL
Length	280
Mol. Wt	31.934 kDa
pI	3.5 (calculated)
Extinction coefficient	16,390 - 16,765 (calc based on 11 Y, 0 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	7..72	PF01842 ACT domain	PMID:19920124
Domain	85..262	PF00551 Formyl transferase	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=purU taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129193	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945827	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004138	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P37051	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11819	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11819	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945827	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001751	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1766	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.68E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	5277	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	2324	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1671	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
purU
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1287827..1287867 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1232 (EcoliWiki Page)	NCBI GEO profiles for purU
microarray	GenExpDB:b1232 (EcoliWiki Page)	Summary of data for purU from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to purU Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11819	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1766	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1232
EcoGene	EcoGene:EG11819	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001751	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004138	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT5G47435 (score: 1.000; bootstrap: 100%) AT4G17360 (score: 0.804)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000015276 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Shigella flexneri	PURU	From SHIGELLACYC
E. coli O157	PURU	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00551 Formyl transferase
Pfam (EcoliWiki Page)	PF01842 ACT domain
Panther (EcoliWiki Page)	PTHR10520:SF7
Superfamily (EcoliWiki Page)	SUPERFAMILY:53328
Superfamily (EcoliWiki Page)	SUPERFAMILY:55021
EcoCyc	EcoCyc:EG11819	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11819	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001751	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1766	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004138	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

purU:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to purU Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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