proS:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

proS

Gene Synonym(s)

ECK0194, b0194, JW0190, drp, drpA[1], drpA

Product Desc.

prolyl-tRNA synthetase[2][3];

Component of prolyl-tRNA synthetase[2][3]

Proline--tRNA ligase[4]

Product Synonyms(s)

prolyl-tRNA synthetase[1], DrpA[2][1], B0194[2][1], Drp[2][1], ProS[2][1] , drpA, ECK0194, JW0190, b0194

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): proS[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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An insertion domain (248-393) in ProS is homologous to YeaK and YbaK.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

proS

Mnemonic

Proline

Synonyms

ECK0194, b0194, JW0190, drp, drpA[1], drpA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

4.68 minutes 

MG1655: 218775..217057
<gbrowseImage> name=NC_000913:217057..218775 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 221616..219898
<gbrowseImage> name=NC_012967:219898..221616 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 217056..218774
<gbrowseImage> name=NC_012759:217056..218774 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 218775..217057
<gbrowseImage> name=NC_007779:217057..218775 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 192879..191161
<gbrowseImage> name=NC_010473:191161..192879 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

217057

Edman degradation

PMID:2203971
PMID:9298646


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

proSK279A

K279A

Severely affects the posttransfer editing activity, with little change in both the aminoacylation and the pretransfer editing activities

seeded from UniProt:P16659

proST257A

T257A

Reduces the posttransfer editing activity 5-fold, with little change in both the aminoacylation and the pretransfer editing activities

seeded from UniProt:P16659

proSD394A

D394A

Reduces the posttransfer editing activity approximately 2-fold, with little change in both the aminoacylation and the pretransfer editing activities

seeded from UniProt:P16659

proSC443G

C443G

150-fold decrease in posttransfer editing activity against alanine, without change neither in pretransfer editing nor in aminoacylation

seeded from UniProt:P16659

proSD350A

D350A

Abolishes the pretransfer editing activity and reduces the aminoacylation activity 20-fold and the posttransfer editing activity 5-fold

seeded from UniProt:P16659

proSH369A

H369A

Reduces both the aminoacylation and the pretransfer editing activities 2.5- fold, and the posttransfer editing activity 5-fold. Loss of specificity in deacylation

seeded from UniProt:P16659

proSD378A

D378A

Little change in both the aminoacylation and the editing activities

seeded from UniProt:P16659

proSD386A

D386A

Reduces the posttransfer editing activity approximately 2-fold, with little change in both the aminoacylation and the pretransfer editing activities

seeded from UniProt:P16659

proS127(ts)

temperature sensitive

PMID:6991867

CGSC:8622


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0190

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCGTACTAGCCAATACCTGCT

Primer 2:CCGCCTTTAATCTGTTTCACCAG

6F5

Kohara Phage

Genobase

PMID:3038334

10F9

Kohara Phage

Genobase

PMID:3038334

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[5]

est. P1 cotransduction: 2% [6]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[5]

est. P1 cotransduction: 59% [6]
Synonyms:zae-502::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10770

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10770

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:949116

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000761

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0763

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000657

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ProS

Synonyms

prolyl-tRNA synthetase[1], DrpA[2][1], B0194[2][1], Drp[2][1], ProS[2][1] , drpA, ECK0194, JW0190, b0194

Product description

prolyl-tRNA synthetase[2][3];

Component of prolyl-tRNA synthetase[2][3]

Proline--tRNA ligase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002314

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002316

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004500

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006195

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0004827

proline-tRNA ligase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01569

F

Seeded from EcoCyc (v14.0)

complete

GO:0004827

proline-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002316

F

Seeded from EcoCyc (v14.0)

complete

GO:0004827

proline-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004500

F

Seeded from EcoCyc (v14.0)

complete

GO:0004827

proline-tRNA ligase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:6.1.1.15

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01569

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002314

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002316

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004154

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004500

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006195

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002314

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002316

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004154

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004500

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006195

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0648

P

Seeded from EcoCyc (v14.0)

complete

GO:0006433

prolyl-tRNA aminoacylation

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01569

P

Seeded from EcoCyc (v14.0)

complete

GO:0006433

prolyl-tRNA aminoacylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002316

P

Seeded from EcoCyc (v14.0)

complete

GO:0006433

prolyl-tRNA aminoacylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004500

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of prolyl-tRNA synthetase

could be indirect

Protein

aceE

PMID:15690043

Experiment(s):EBI-888737

Protein

dnaN

PMID:15690043

Experiment(s):EBI-888737

Protein

groL

PMID:16606699

Experiment(s):EBI-1135910
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MRTSQYLLST LKETPADAEV ISHQLMLRAG MIRKLASGLY TWLPTGVRVL KKVENIVREE
MNNAGAIEVS MPVVQPADLW QESGRWEQYG PELLRFVDRG ERPFVLGPTH EEVITDLIRN
ELSSYKQLPL NFYQIQTKFR DEVRPRFGVM RSREFLMKDA YSFHTSQESL QETYDAMYAA
YSKIFSRMGL DFRAVQADTG SIGGSASHEF QVLAQSGEDD VVFSDTSDYA ANIELAEAIA
PKEPRAAATQ EMTLVDTPNA KTIAELVEQF NLPIEKTVKT LLVKAVEGSS FPQVALLVRG
DHELNEVKAE KLPQVASPLT FATEEEIRAV VKAGPGSLGP VNMPIPVVID RTVAAMSDFA
AGANIDGKHY FGINWDRDVA TPEVADIRNV VAGDPSPDGQ GRLLIKRGIE VGHIFQLGTK
YSEALKASVQ GEDGRNQILT MGCYGIGVTR VVAAAIEQNY DERGIVWPDA IAPFQVAILP
MNMHKSFRVQ ELAEKLYSEL RAQGIEVLLD DRKERPGVMF ADMELIGIPH TIVLGDRNLD
NDDIEYKYRR NGEKQLIKTG DIVEYLVKQI KG
Length

572

Mol. Wt

63.692 kDa

pI

5.0 (calculated)

Extinction coefficient

54,320 - 54,445 (calc based on 18 Y, 5 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

475..569

PF03129 Anticodon binding domain

PMID:19920124

Domain

49..219

PF00587 tRNA synthetase class II core domain (G, H, P, S and T)

PMID:19920124

Domain

257..379

PF04073 YbaK / prolyl-tRNA synthetases associated domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=proS taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128187

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:949116

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000657

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P16659

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10770

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10770

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:949116

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000761

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0763

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

2.35E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

12156

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2265

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

5518

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

proS

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:218755..218795 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0194 (EcoliWiki Page)

NCBI GEO profiles for proS

microarray

GenExpDB:b0194 (EcoliWiki Page)

Summary of data for proS from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (218706..218951) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ10; Well:B5[7]

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Notes

Accessions Related to proS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10770

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0763

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0194

EcoGene

EcoGene:EG10770

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000761

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000657

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000011343 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G52520 (score: 1.000; bootstrap: 100%)
  • AT3G62120 (score: 0.288)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00041965 (score: 1.000; bootstrap: 98%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00004190 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000028036 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000001808 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-030131-4219 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-040425-1283 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0231314 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0027082 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA11463-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000017526 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000294375 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Mus musculus

  • MGI:2386296 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q7XID8 (score: 1.000; bootstrap: 79%)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000001345 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000009630 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YER087W (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPBC24C63 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000156226 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00033608001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000043241 (score: 1.000; bootstrap: 98%)

From Inparanoid:20070104

Shigella flexneri

PROS

From SHIGELLACYC

E. coli O157

PROS

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF03129 Anticodon binding domain

Panther (EcoliWiki Page)

PTHR11451:SF3

Superfamily (EcoliWiki Page)

SUPERFAMILY:52954

Superfamily (EcoliWiki Page)

SUPERFAMILY:55681

Superfamily (EcoliWiki Page)

SUPERFAMILY:55826

Pfam (EcoliWiki Page)

PF00587 tRNA synthetase class II core domain (G, H, P, S and T)

Pfam (EcoliWiki Page)

PF04073 YbaK / prolyl-tRNA synthetases associated domain

EcoCyc

EcoCyc:EG10770

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10770

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000761

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0763

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000657

Escherichia coli str. K-12 substr. MG1655

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  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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