proS:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
proS |
|---|---|
| Mnemonic |
Proline |
| Synonyms |
ECK0194, b0194, JW0190, drp, drpA[1], drpA |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
4.68 minutes |
MG1655: 218775..217057 |
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NC_012967: 221616..219898 |
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NC_012759: 217056..218774 |
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W3110 |
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W3110: 218775..217057 |
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DH10B: 192879..191161 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
217057 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
proSK279A |
K279A |
Severely affects the posttransfer editing activity, with little change in both the aminoacylation and the pretransfer editing activities |
seeded from UniProt:P16659 | ||||
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proST257A |
T257A |
Reduces the posttransfer editing activity 5-fold, with little change in both the aminoacylation and the pretransfer editing activities |
seeded from UniProt:P16659 | ||||
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proSD394A |
D394A |
Reduces the posttransfer editing activity approximately 2-fold, with little change in both the aminoacylation and the pretransfer editing activities |
seeded from UniProt:P16659 | ||||
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proSC443G |
C443G |
150-fold decrease in posttransfer editing activity against alanine, without change neither in pretransfer editing nor in aminoacylation |
seeded from UniProt:P16659 | ||||
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proSD350A |
D350A |
Abolishes the pretransfer editing activity and reduces the aminoacylation activity 20-fold and the posttransfer editing activity 5-fold |
seeded from UniProt:P16659 | ||||
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proSH369A |
H369A |
Reduces both the aminoacylation and the pretransfer editing activities 2.5- fold, and the posttransfer editing activity 5-fold. Loss of specificity in deacylation |
seeded from UniProt:P16659 | ||||
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proSD378A |
D378A |
Little change in both the aminoacylation and the editing activities |
seeded from UniProt:P16659 | ||||
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proSD386A |
D386A |
Reduces the posttransfer editing activity approximately 2-fold, with little change in both the aminoacylation and the pretransfer editing activities |
seeded from UniProt:P16659 | ||||
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proS127(ts) |
temperature sensitive |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0190 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCGTACTAGCCAATACCTGCT Primer 2:CCGCCTTTAATCTGTTTCACCAG | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 2% [8] | ||
|
Linked marker |
est. P1 cotransduction: 59% [8] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Eriani, G et al. (1990) Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs. Nature 347 203-6 PubMed
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ Bohman, K & Isaksson, LA (1980) A temperature-sensitive mutant in prolinyl-tRNA ligase of Escherichia coli K-12. Mol. Gen. Genet. 177 603-5 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
