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On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	proB
Gene Synonym(s)	ECK0243, b0242, JW0232, pro2, pro(2)^[1], pro
Product Desc.	ProB^[2]^[3]; Component of γ-glutamyl kinase^[2]^[3]; γ-glutamyl kinase-GP-reductase multienzyme complex^[2]^[3] Glutamate 5-kinase, proline biosynthesis^[4]
Product Synonyms(s)	gamma-glutamate kinase^[1], B0242^[2]^[1], ProB^[2]^[1] , ECK0243, JW0232, pro, b0242
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): proBA^[2]
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Contains PUA domain, required for proline-triggered aggregation.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	proB
Mnemonic	Proline
Synonyms	ECK0243, b0242, JW0232, pro2, pro(2)^[1], pro
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	5.6 minutes	MG1655: 259612..260715 <gbrowseImage> name=NC_000913:259612..260715 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 264880..265983 <gbrowseImage> name=NC_012967:264880..265983 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 259611..260714 <gbrowseImage> name=NC_012759:259611..260714 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 259612..260715 <gbrowseImage> name=NC_007779:259612..260715 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 233716..234819 <gbrowseImage> name=NC_010473:233716..234819 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	259612	Edman degradation	PMID:6089111
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔproB (Keio:JW0232)	deletion	deletion	Auxotrophies	Requires proline for growth	PMID:16738554	Shigen CGSC8467^[5]
proB22			Auxotrophies	Requires proline for growth		CGSC:5455
proB28			Auxotrophies	Requires proline for growth		CGSC:10032
proB91			Auxotrophies	Requires proline for growth		CGSC:12266
ΔproB760::kan	deletion	deletion	Auxotrophies	Requires proline for growth	PMID:16738554	CGSC:102468
proB			Resistant to	Nitro-4-pyridine N-oxide Resistance	PMID:385587	Strain: CU210
proB			Resistant to	Nitro-4-pyridine N-oxide Resistance	PMID:5329291	Strain: W3630	Table 1
proB			Resistant to	Nitro-4-pyridine N-oxide Resistance	PMID:4598010
proB			Resistant to	Dehydroproline Resistance	PMID:4598010
proB			Resistant to	Azetidine carboxylate resistance	PMID:385587
proB			Resistant to	Nitro-4-pyridine N-oxide Resistance	PMID:4598010
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0232	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGTGACAGCCAGACGCTGGT Primer 2:CCACGGGTAATCATGTCATCACG
8F9	Kohara Phage	Genobase	PMID:3038334
8G4	Kohara Phage	Genobase	PMID:3038334
yafC502::Tn10	Linked marker	CAG18436 = CGSC7328^[5]	est. P1 cotransduction: 35% ^[6] Synonyms:zae-502::Tn10
proA81::Tn10	Linked marker	CAG18447 = CGSC7330^[5]	est. P1 cotransduction: 99% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10768	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10768	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000759	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946425	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0761	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000830	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	ProB
Synonyms	gamma-glutamate kinase^[1], B0242^[2]^[1], ProB^[2]^[1] , ECK0243, JW0232, pro, b0242
Product description	ProB^[2]^[3]; Component of γ-glutamyl kinase^[2]^[3]; γ-glutamyl kinase-GP-reductase multienzyme complex^[2]^[3] Glutamate 5-kinase, proline biosynthesis^[4]
EC number (for enzymes)	2.7.2.11^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	PMID:16337196	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	PMID:17069808	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	PMID:6319365	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0003723	RNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002478	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004349	glutamate 5-kinase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00456	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004349	glutamate 5-kinase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001057	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004349	glutamate 5-kinase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005715	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004349	glutamate 5-kinase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011529	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004349	glutamate 5-kinase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR019797	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004349	glutamate 5-kinase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.7.2.11	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004349	glutamate 5-kinase activity	PMID:16337196	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0004349	glutamate 5-kinase activity	PMID:17069808	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0004349	glutamate 5-kinase activity	PMID:6319365	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00456	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005715	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011529	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006561	proline biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00456	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006561	proline biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001057	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006561	proline biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005715	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006561	proline biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011529	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006561	proline biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR019797	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006561	proline biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0641	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006561	proline biosynthetic process	PMID:2841193	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of γ-glutamyl kinase	could be indirect
Protein	prs		PMID:16606699	Experiment(s):EBI-1136004
Protein	rfaS		PMID:16606699	Experiment(s):EBI-1136004
Protein	Subunits of γ-glutamyl kinase-GP-reductase multienzyme complex	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSDSQTLVVK LGTSVLTGGS RRLNRAHIVE LVRQCAQLHA AGHRIVIVTS GAIAAGREHL GYPELPATIA SKQLLAAVGQ SRLIQLWEQL FSIYGIHVGQ MLLTRADMED RERFLNARDT LRALLDNNIV PVINENDAVA TAEIKVGDND NLSALAAILA GADKLLLLTD QKGLYTADPR SNPQAELIKD VYGIDDALRA IAGDSVSGLG TGGMSTKLQA ADVACRAGID TIIAAGSKPG VIGDVMEGIS VGTLFHAQAT PLENRKRWIF GAPPAGEITV DEGATAAILE RGSSLLPKGI KSVTGNFSRG EVIRICNLEG RDIAHGVSRY NSDALRRIAG HHSQEIDAIL GYEYGPVAVH RDDMITR
Length	367
Mol. Wt	39.057 kDa
pI	6.5 (calculated)
Extinction coefficient	21,430 - 21,805 (calc based on 7 Y, 2 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	5..235	PF00696 Amino acid kinase family	PMID:19920124
Domain	276..350	PF01472 PUA domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=proB taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128228	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946425	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000830	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A7B5	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10768	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10768	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946425	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000759	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0761	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.78E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	2048	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	536	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1202	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
proBA
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:259592..259632 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0242 (EcoliWiki Page)	NCBI GEO profiles for proB
microarray	GenExpDB:b0242 (EcoliWiki Page)	Summary of data for proB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (259024..259471) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ01; Well:A7^[7]
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Notes

Accessions Related to proB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10768	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0761	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0242
EcoGene	EcoGene:EG10768	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000759	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000830	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Saccharomyces cerevisiae	YDR300C (score: 1.000; bootstrap: 100%) YHR033W (score: 0.572)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC17H93c (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000013948 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Shigella flexneri	PROB	From SHIGELLACYC
E. coli O157	PROB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01472 PUA domain
Pfam (EcoliWiki Page)	PF00696 Amino acid kinase family
Superfamily (EcoliWiki Page)	SUPERFAMILY:53633
Panther (EcoliWiki Page)	PTHR11063:SF6
Superfamily (EcoliWiki Page)	SUPERFAMILY:88697
EcoCyc	EcoCyc:EG10768	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10768	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000759	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0761	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000830	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

proB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to proB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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