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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

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Standard Name	pncB
Gene Synonym(s)	ECK0922, b0931, JW0914^[1], JW0914
Product Desc.	Nicotinate phosphoribosyltransferase^[2]
Product Synonyms(s)	nicotinate phosphoribosyltransferase^[1], B0931^[3]^[1], PncB^[3]^[1] , ECK0922, JW0914, b0931
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): pncB^[3], OP00273
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	pncB
Mnemonic	Pyridine nucleotide cycle
Synonyms	ECK0922, b0931, JW0914^[1], JW0914
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	21.3 minutes	MG1655: 989579..988377 <gbrowseImage> name=NC_000913:988377..989579 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1007447..1006245 <gbrowseImage> name=NC_012967:1006245..1007447 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 891345..892547 <gbrowseImage> name=NC_012759:891345..892547 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 990778..989576 <gbrowseImage> name=NC_007779:989576..990778 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1043507..1042305 <gbrowseImage> name=NC_010473:1042305..1043507 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	988380	Edman degradation	PMID:2211655
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔpncB (Keio:JW0914)	deletion	deletion			PMID:16738554	Shigen CGSC8926^[4]
pncB::Tn5KAN-2 (FB20253)	Insertion at nt 926 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20253	does not contain pKD46
ΔpncB747::kan					PMID:16738554	CGSC:102388
pncB^-			Resistant to	Resistant to 6-amino-nicotinamide	CURRENT MICROBIOLOGY Volume 4, Number 1 (1980), 31-35, DOI: 10.1007/BF02602888	Strain: UTH7095	Table 1
pncB^-			Resistant to	Resistant to 6-amino-nicotinate	CURRENT MICROBIOLOGY Volume 4, Number 1 (1980), 31-35, DOI: 10.1007/BF02602888	Strain: UTH7095	Table 1
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0914	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACACAATTCGCTTCTCCTGT Primer 2:CCACTGGCTTTTTTAATATGCGG
11F5	Kohara Phage	Genobase	PMID:3038334
7F5	Kohara Phage	Genobase	PMID:3038334
zca-1230::Tn10	Linked marker	CAG18478 = CGSC7356^[4]	est. P1 cotransduction: 17% ^[5] Synonyms:zbj-1230::Tn10
zcb-3059::Tn10	Linked marker	CAG12094 = CGSC7358^[4]	est. P1 cotransduction: 62% ^[5]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10742	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10742	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000733	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946648	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0735	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003167	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PncB
Synonyms	nicotinate phosphoribosyltransferase^[1], B0931^[3]^[1], PncB^[3]^[1] , ECK0922, JW0914, b0931
Product description	Nicotinate phosphoribosyltransferase^[2]
EC number (for enzymes)	2.4.2.11^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004516	nicotinate phosphoribosyltransferase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00570	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004516	nicotinate phosphoribosyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006406	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004516	nicotinate phosphoribosyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007229	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004516	nicotinate phosphoribosyltransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.4.2.11	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00570	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009435	NAD biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00570	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016757	transferase activity, transferring glycosyl groups	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0328	F	Seeded from EcoCyc (v14.0)	complete
	GO:0019357	nicotinate nucleotide biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006406	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019363	pyridine nucleotide biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007229	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019363	pyridine nucleotide biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0662	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rplS	PMID:15690043	Experiment(s):EBI-887088
Protein	rplV	PMID:15690043	Experiment(s):EBI-887088
Protein	rpsB	PMID:15690043	Experiment(s):EBI-887088
Protein	tufA	PMID:15690043	Experiment(s):EBI-887088
Protein	yeiM	PMID:15690043	Experiment(s):EBI-887088
Protein	zwf	PMID:16606699	Experiment(s):EBI-1138406
Protein	ykgF	PMID:16606699	Experiment(s):EBI-1138406
Protein	rrmA	PMID:16606699	Experiment(s):EBI-1138406
Protein	uspG	PMID:16606699	Experiment(s):EBI-1138406
Protein	rplB	PMID:16606699	Experiment(s):EBI-1138406
Protein	hchA	PMID:16606699	Experiment(s):EBI-1138406
Protein	nadE	PMID:16606699	Experiment(s):EBI-1138406
Protein	groL	PMID:16606699	Experiment(s):EBI-1138406
Protein	pepD	PMID:16606699	Experiment(s):EBI-1138406
Protein	psuT	PMID:19402753	LCMS(ID Probability):99.0
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTQFASPVLH SLLDTDAYKL HMQQAVFHHY YDVHVAAEFR CRGDDLLGIY ADAIREQVQA MQHLRLQDDE YQWLSALPFF KADYLNWLRE FRFNPEQVTV SNDNGKLDIR LSGPWREVIL WEVPLLAVIS EMVHRYRSPQ ADVAQALDTL ESKLVDFSAL TAGLDMSRFH LMDFGTRRRF SREVQETIVK RLQQESWFVG TSNYDLARRL SLTPMGTQAH EWFQAHQQIS PDLANSQRAA LAAWLEEYPD QLGIALTDCI TMDAFLRDFG VEFASRYQGL RHDSGDPVEW GEKAIAHYEK LGIDPQSKTL VFSDNLDLRK AVELYRHFSS RVQLSFGIGT RLTCDIPQVK PLNIVIKLVE CNGKPVAKLS DSPGKTICHD KAFVRALRKA FDLPHIKKAS
Length	400
Mol. Wt	45.897 kDa
pI	6.7 (calculated)
Extinction coefficient	61,880 - 62,505 (calc based on 12 Y, 8 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P18133
Domain	169..395	PF04095 Nicotinate phosphoribosyltransferase (NAPRTase) family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pncB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128898	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946648	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003167	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P18133	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10742	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10742	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946648	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000733	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0735	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	2.68E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	27.614+/-0.249	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.101081531	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	2424	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1038	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1310	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
pncB
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:989559..989599 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0931 (EcoliWiki Page)	NCBI GEO profiles for pncB
microarray	GenExpDB:b0931 (EcoliWiki Page)	Summary of data for pncB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (989499..989850) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ04; Well:G1^[6]
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Notes

Accessions Related to pncB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10742	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0735	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0931
EcoGene	EcoGene:EG10742	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000733	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003167	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000000304 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YOR209C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC14866 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000012970 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	PNCB	From SHIGELLACYC
E. coli O157	PNCB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04095 Nicotinate phosphoribosyltransferase (NAPRTase) family
Superfamily (EcoliWiki Page)	SUPERFAMILY:51690
Panther (EcoliWiki Page)	PTHR11098:SF1
Superfamily (EcoliWiki Page)	SUPERFAMILY:54675
EcoCyc	EcoCyc:EG10742	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10742	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000733	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0735	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003167	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

pncB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to pncB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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