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On One Page

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Expression|

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Standard Name	phoU
Gene Synonym(s)	ECK3717, b3724, JW3702, phoT, pst, nmpA^[1]^[2]
Product Desc.	PhoU phosphate transport system protein^[2]^[3] Negative regulator of pho regulon; may have additional role in phosphate metabolism^[4]
Product Synonyms(s)	negative regulator of PhoA/PhoB two-component regulatory system^[1], B3724^[2]^[1], Pst^[2]^[1], PhoT^[2]^[1], NmpA^[2]^[1], PhoU^[2]^[1] , ECK3717, JW3702, phoT, b3724
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): pstSCAB-phoU^[2], OP00284, phoT, pstSCABphoU
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	phoU
Mnemonic	Phosphate
Synonyms	ECK3717, b3724, JW3702, phoT, pst, nmpA^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	84.16 minutes	MG1655: 3905601..3904876 <gbrowseImage> name=NC_000913:3904876..3905601 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3867945..3867220 <gbrowseImage> name=NC_012967:3867220..3867945 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3793209..3793934 <gbrowseImage> name=NC_012759:3793209..3793934 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3729103..3729828 <gbrowseImage> name=NC_007779:3729103..3729828 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4003185..4002460 <gbrowseImage> name=NC_010473:4002460..4003185 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3904876	Edman degradation	PMID:3536855
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
phoU(del) (Keio:JW3702)	deletion	deletion			PMID:16738554	Shigen CGSC10706^[5]
pst-32						CGSC:6402
pst-9						CGSC:11618
phoU755(del)::kan					PMID:16738554	CGSC:102364
phoU755(del)::FRTKanFRT			Mutagenesis rate	Decreased stress-induced mutagenesis (SIM) phenotype.	PMID:23224554	Parental Strain: SMR4562 Experimental Strain: SMR13473	Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population.
CAG45114 phoU755(del)::FRTKanFRT	deletion		Sigma^E activity	Decrease in sigma^E activity	PMID:23224554	Parental Strain: CAG45114 Experimental Strain: SMR15289	See table S11 for full experimental results.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3702	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGACAGTCTCAATCTTAATAA Primer 2:CCTTTGTCGCTATCTTTCCCCGC
7A1	Kohara Phage	Genobase	PMID:3038334
zid-501::Tn10	Linked marker	CAG18499 = CGSC7458^[5]	est. P1 cotransduction: 30% ^[6]
rbsD296::Tn10	Linked marker	CAG18501 = CGSC7460^[5]	est. P1 cotransduction: 39% ^[6] Synonyms:zie-296::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10735	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10735	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000726	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948233	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0728	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012177	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PhoU
Synonyms	negative regulator of PhoA/PhoB two-component regulatory system^[1], B3724^[2]^[1], Pst^[2]^[1], PhoT^[2]^[1], NmpA^[2]^[1], PhoU^[2]^[1] , ECK3717, JW3702, phoT, b3724
Product description	PhoU phosphate transport system protein^[2]^[3] Negative regulator of pho regulon; may have additional role in phosphate metabolism^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006817	phosphate transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0592	P	Seeded from EcoCyc (v14.0)	complete
	GO:0045936	negative regulation of phosphate metabolic process	PMID:1459954	IMP: Inferred from Mutant Phenotype		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	glpD		PMID:16606699	Experiment(s):EBI-1146579
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MDSLNLNKHI SGQFNAELES IRTQVMTMGG MVEQQLSDAI TAMHNQDSDL AKRVIEGDKN VNMMEVAIDE ACVRIIAKRQ PTASDLRLVM VISKTIAELE RIGDVADKIC RTALEKFSQQ HQPLLVSLES LGRHTIQMLH DVLDAFARMD IDEAVRIYRE DKKVDQEYEG IVRQLMTYMM EDSRTIPSVL TALFCARSIE RIGDRCQNIC EFIFYYVKGQ DFRHVGGDEL DKLLAGKDSD K
Length	241
Mol. Wt	27.417 kDa
pI	5.0 (calculated)
Extinction coefficient	7,450 - 8,075 (calc based on 5 Y, 0 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	25..113	PF01895 PhoU domain	PMID:19920124
Domain	128..213	PF01895 PhoU domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=phoU taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131592	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948233	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012177	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A9K7	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10735	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10735	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948233	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000726	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0728	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	1185	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	797	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1426	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
pstSCAB-phoU
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3905581..3905621 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3724 (EcoliWiki Page)	NCBI GEO profiles for phoU
microarray	GenExpDB:b3724 (EcoliWiki Page)	Summary of data for phoU from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to phoU Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10735	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0728	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3724
EcoGene	EcoGene:EG10735	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000726	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012177	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000033404 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	PHOU	From SHIGELLACYC
E. coli O157	PHOU	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR10010:SF14
Superfamily (EcoliWiki Page)	SUPERFAMILY:109755
Pfam (EcoliWiki Page)	PF01895 PhoU domain
Pfam (EcoliWiki Page)	PF01895 PhoU domain
EcoCyc	EcoCyc:EG10735	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10735	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000726	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0728	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012177	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

phoU:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to phoU Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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