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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	opgG
Gene Synonym(s)	ECK1034, b1048, JW1035, opgG, mdoA, G428, mdoG^[1]^[2], mdoG
Product Desc.	periplasmic glucan (MDO) biosynthesis protein^[2]^[3]
Product Synonyms(s)	glucan biosynthesis protein, periplasmic^[1], MdoA^[2]^[1], B1048^[2]^[1], MdoG^[2]^[1], OpgG^[2]^[1] , ECK1034, JW1035, mdoA, mdoG, b1048
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): opgGH^[2]
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	opgG
Mnemonic	Osmoregulated periplasmic glucans
Synonyms	ECK1034, b1048, JW1035, opgG, mdoA, G428, mdoG^[1]^[2], mdoG
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	23.89 minutes	MG1655: 1108558..1110093 <gbrowseImage> name=NC_000913:1108558..1110093 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1123904..1125439 <gbrowseImage> name=NC_012967:1123904..1125439 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1012483..1014018 <gbrowseImage> name=NC_012759:1012483..1014018 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1110912..1112447 <gbrowseImage> name=NC_007779:1110912..1112447 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1164003..1165538 <gbrowseImage> name=NC_010473:1164003..1165538 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1108624	Edman degradation	PMID:9298646
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔmdoG (Keio:JW1035)	deletion	deletion	PMID:16738554	Shigen CGSC9000^[4]
mdoG::Tn5KAN-2 (FB21169)	Insertion at nt 693 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20091	does not contain pKD46
ΔmdoG790::kan			PMID:16738554	CGSC:101782
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1035	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATGAAAATGCGTTGGTTGAG Primer 2:CCTTCATTGGCAGGTAACTGGTA
1C7	Kohara Phage	Genobase	PMID:3038334
1H7	Kohara Phage	Genobase	PMID:3038334
zcc-282::Tn10	Linked marker	CAG18466 = CGSC7359^[4]	est. P1 cotransduction: 13% ^[5]
zce-726::Tn10	Linked marker	CAG12078 = CGSC7361^[4]	est. P1 cotransduction: 12% ^[5]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11885	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1831	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1048
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C1313
EcoGene	EcoGene:EG11885	Escherichia coli str. K-12 substr. MG1655
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:MDOG
CGSC (EcoliWiki Page)	CGSC:31849
ASAP	ASAP:ABE-0003558	Escherichia coli str. K-12 substr. MG1655
EcoCyc (EcoliWiki Page)	ECOCYC:EG11885	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945005	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001815	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	OpgG
Synonyms	glucan biosynthesis protein, periplasmic^[1], MdoA^[2]^[1], B1048^[2]^[1], MdoG^[2]^[1], OpgG^[2]^[1] , ECK1034, JW1035, mdoA, mdoG, b1048
Product description	periplasmic glucan (MDO) biosynthesis protein^[2]^[3]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011013	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011013	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009250	glucan biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01069	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016051	carbohydrate biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007444	P	Seeded from EcoCyc (v14.0)	complete
	GO:0030246	carbohydrate binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011013	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01069	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007444	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	trxB	PMID:16606699	Experiment(s):EBI-1138688
Protein	rpsC	PMID:16606699	Experiment(s):EBI-1138688
Protein	ytfN	PMID:16606699	Experiment(s):EBI-1138688
Protein	rpsI	PMID:16606699	Experiment(s):EBI-1138688
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
periplasm		From EcoCyc^[3]
Periplasm	PMID:9298646	EchoLocation:mdoG
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMKMRWLSAA VMLTLYTSSS WAFSIDDVAK QAQSLAGKGY ETPKSNLPSV FRDMKYADYQ QIQFNHDKAY WNNLKTPFKL EFYHQGMYFD TPVKINEVTA TAVKRIKYSP DYFTFGDVQH DKDTVKDLGF AGFKVLYPIN SKDKNDEIVS MLGASYFRVI GAGQVYGLSA RGLAIDTALP SGEEFPRFKE FWIERPKPTD KRLTIYALLD SPRATGAYKF VVMPGRDTVV DVQSKIYLRD KVGKLGVAPL TSMFLFGPNQ PSPANNYRPE LHDSNGLSIH AGNGEWIWRP LNNPKHLAVS SFSMENPQGF GLLQRGRDFS RFEDLDDRYD LRPSAWVTPK GEWGKGSVEL VEIPTNDETN DNIVAYWTPD QLPEPGKEMN FKYTITFSRD EDKLHAPDNA WVQQTRRSTG DVKQSNLIRQ PDGTIAFVVD FTGAEMKKLP EDTPVTAQTS IGDNGEIVES TVRYNPVTKG WRLVMRVKVK DAKKTTEMRA ALVNADQTLS ETWSYQLPAN E
Length	511
Mol. Wt	57.913 kDa
pI	7.1 (calculated)
Extinction coefficient	97,290 (calc based on 21 Y, 12 W, and C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-22	UniProt Manual:Signal Peptides	UniProt:P33136
Domain	23..509	PF04349 Periplasmic glucan biosynthesis protein, MdoG		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mdoG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129011	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945005	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003558	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P33136	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11885	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11885	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945005	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001815	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1831	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.51E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	4229	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1637	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	2570	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mdoGH
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1108538..1108578 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1048 (EcoliWiki Page)	NCBI GEO profiles for mdoG
microarray	GenExpDB:b1048 (EcoliWiki Page)	Summary of data for mdoG from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to opgG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11885	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1831	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1048
EcoGene	EcoGene:EG11885	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001815	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003558	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	MDOG	From SHIGELLACYC
E. coli O157	MDOG	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04349 Periplasmic glucan biosynthesis protein, MdoG
Superfamily (EcoliWiki Page)	SUPERFAMILY:74650
Superfamily (EcoliWiki Page)	SUPERFAMILY:81296
EcoCyc	EcoCyc:EG11885	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11885	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001815	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1831	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003558	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

opgG:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to opgG Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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