nuoK:On One Page

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Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

nuoK

Gene Synonym(s)

ECK2273, b2279, JW2274[1], JW2274

Product Desc.

NuoK[2][3];

Component of NADH dehydrogenase I[2][3]

NADH:ubiquinone oxidoreductase subunit K, complex I; NADH dehydrogenase I[4]

Product Synonyms(s)

NADH:ubiquinone oxidoreductase, membrane subunit K[1], B2279[2][1], NuoK[2][1] , ECK2273, JW2274, b2279

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): nuoABCEFGHIJKLMN[2], nuo1-14

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

nuoK

Mnemonic

NADH:ubiquinone oxidoreductase

Synonyms

ECK2273, b2279, JW2274[1], JW2274

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

51.58 minutes 

MG1655: 2393367..2393065
<gbrowseImage> name=NC_000913:2393065..2393367 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2338936..2338634
<gbrowseImage> name=NC_012967:2338634..2338936 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2278870..2279172
<gbrowseImage> name=NC_012759:2278870..2279172 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2400015..2399713
<gbrowseImage> name=NC_007779:2399713..2400015 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2485132..2484830
<gbrowseImage> name=NC_010473:2484830..2485132 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

nuoK(del) (Keio:JW2274)

deletion

deletion

PMID:16738554

Shigen
CGSC8304[5]

nuoKE72A

E72A

Retains 43% dNADH oxidase and dNADH-DB reductase activity

seeded from UniProt:P0AFE4

nuoKE72Q

E72Q

Retains 22% dNADH oxidase and dNADH-DB reductase activity

seeded from UniProt:P0AFE4

nuoKRR25AA

RR25AA

Retains 14% dNADH oxidase and dNADH-DB reductase activity

seeded from UniProt:P0AFE4

nuoKE36A

E36A

Retains 2% dNADH oxidase and dNADH- DB reductase activity

seeded from UniProt:P0AFE4

nuoKE36Q

E36Q

Retains 2% dNADH oxidase and dNADH- DB reductase activity, neither a membrane potential nor a proton gradient can be formed by isolated membranes

seeded from UniProt:P0AFE4

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire b-Methyl-D-glucoside

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire a-D-Glucose-6-phosphate

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire D-Galacturonate

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire Glucuronamide

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanyl-glycine

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire Glycyl-L-aspartate

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938

nuoK(del)::kan

deletion

Biolog:respiration

unable to respire Uridine

PMID:16095938

nuoK761(del)::kan

PMID:16738554

CGSC:99827

nuoK761(del)::FRT

deletion

Mutagenesis rate.

Decreased Stress Induced Mutagenesis (SIM).

PMID:23224554

Parent Strain: SMR4562 Experimental Strain: SMR13653

The mutation's effect on SIM was of Medium strength with a reduction of mutant frequency of 68 to 89 percent. See table S3 for full experimental data.

nuoK761(del)::FRT

deletion

SDS-EDTA Sensitivity

PMID:23224554

Parent Strain: SMR4562 Experimental Strain: SMR13653

The mutation conferred an increase in SDS-EDTA sensitivity. See table S7 and S1 for a summary of experimental data.

SMR4562 yiaG-yfp FRTcatFRT nuoK761(del)::FRTKanFRT

deletion

SigmaS activity

Decrease in SigmaS activity

PMID:23224554

Parental Strain: SMR10582 Experimental Strain: SMR14285

See table S8 for experimental data.

CAG45114 nuoK761(del)::FRTKanFRT

deletion

SigmaE activity

Decrease in sigmaE activity

PMID:23224554

Parental Strain: CAG45115 Experimental Strain: SMR15366

See table S11 for full experimental data.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2274

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCATCCCCTTACAACATGGACT

Primer 2:CCTCCGCGCATCTCACTTACTGA

4C8

Kohara Phage

Genobase

PMID:3038334

E9B9

Kohara Phage

Genobase

PMID:3038334

zfb-223::Tn10

Linked marker

CAG18484 = CGSC7406[5]

est. P1 cotransduction: 89% [6]
Synonyms:zej-223::Tn10

zfd-1::Tn10

Linked marker

CAG18467 = CGSC7408[5]

est. P1 cotransduction: % [6]
Synonyms:zfb-1::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12091

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12091

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001996

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947580

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2015

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007534

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

NuoK

Synonyms

NADH:ubiquinone oxidoreductase, membrane subunit K[1], B2279[2][1], NuoK[2][1] , ECK2273, JW2274, b2279

Product description

NuoK[2][3];

Component of NADH dehydrogenase I[2][3]

NADH:ubiquinone oxidoreductase subunit K, complex I; NADH dehydrogenase I[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005515

protein binding

PMID:19959836

IPI: Inferred from Physical Interaction

UniProtKB:P25714

F

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

PMID:7607227

IDA: Inferred from Direct Assay

C

The NADH dehydrogenase I complex fractionates with cytoplasmic membranes.

complete

Contributes to

GO:0003954

NADH dehydrogenase activity

PMID:3122832

IDA: Inferred from Direct Assay

F

Purified NADH dehydrogenase I in membrane vesicles can oxidize NADH.

complete

GO:0019684

photosynthesis, light reaction

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01456

P

Seeded from EcoCyc (v14.0)

complete

GO:0030964

NADH dehydrogenase complex

PMID:7607227

IDA: Inferred from Direct Assay

C

E. coli NADH dehydrogenase I was purified and the subunits separated by SDS-PAGE. These seven subunits were not recovered from the gel in sufficient amounts to allow Edman degradation. The polypeptides were assigned to their respective genes based on their apparent molecular masses.

complete

GO:0042773

ATP synthesis coupled electron transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001133

P

Seeded from EcoCyc (v14.0)

complete

GO:0048038

quinone binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0874

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01456

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001133

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of NADH dehydrogenase I

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 3 predicted transmembrane domains

Daley et al. (2005) [7]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MIPLQHGLIL AAILFVLGLT GLVIRRNLLF MLIGLEIMIN ASALAFVVAG SYWGQTDGQV
MYILAISLAA AEASIGLALL LQLHRRRQNL NIDSVSEMRG
Length

100

Mol. Wt

10.845 kDa

pI

9.1 (calculated)

Extinction coefficient

8,480 (calc based on 2 Y, 1 W, and 0 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

6..100

PF00420 NADH-ubiquinone/plastoquinone oxidoreductase chain 4L

PMID:19920124

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<motif_map/>

tmhmm.php?gene=nuoK&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nuoK taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130214

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947580

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007534

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AFE4

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12091

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12091

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947580

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001996

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2015

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

1352

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

592

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

705

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

nuoABCEFGHIJKLMN

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2393347..2393387 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2279 (EcoliWiki Page)

NCBI GEO profiles for nuoK

microarray

GenExpDB:b2279 (EcoliWiki Page)

Summary of data for nuoK from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to nuoK Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12091

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2015

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2279

EcoGene

EcoGene:EG12091

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001996

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007534

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Oryza gramene

  • P69378 (score: 1.000; bootstrap: 62%)
  • Q6QXX2 (score: 1.000; bootstrap: 64%)
  • Q6L3D8 (score: 1.000; bootstrap: 64%)
  • Q6ENP5 (score: 1.000; bootstrap: 63%)
  • P12128 (score: 1.000; bootstrap: 63%)
  • Q6ENA5 (score: 1.000; bootstrap: 61%)
  • P69379 (score: 1.000; bootstrap: 61%)
  • Q6QY35 (score: 1.000; bootstrap: 62%)
  • P11646 (score: 0.977)

From Inparanoid:20070104

Shigella flexneri

NUOK

From SHIGELLACYC

E. coli O157

NUOK

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00420 NADH-ubiquinone/plastoquinone oxidoreductase chain 4L

Panther (EcoliWiki Page)

PTHR11434:SF0

EcoCyc

EcoCyc:EG12091

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12091

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001996

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2015

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007534

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

Categories

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