nuoK:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
nuoK |
|---|---|
| Mnemonic |
NADH:ubiquinone oxidoreductase |
| Synonyms |
ECK2273, b2279, JW2274[1], JW2274 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
51.58 minutes |
MG1655: 2393367..2393065 |
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NC_012967: 2338936..2338634 |
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NC_012759: 2278870..2279172 |
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W3110 |
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W3110: 2400015..2399713 |
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DH10B: 2485132..2484830 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
nuoK(del) (Keio:JW2274) |
deletion |
deletion |
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nuoKE72A |
E72A |
Retains 43% dNADH oxidase and dNADH-DB reductase activity |
seeded from UniProt:P0AFE4 | ||||
|
nuoKE72Q |
E72Q |
Retains 22% dNADH oxidase and dNADH-DB reductase activity |
seeded from UniProt:P0AFE4 | ||||
|
nuoKRR25AA |
RR25AA |
Retains 14% dNADH oxidase and dNADH-DB reductase activity |
seeded from UniProt:P0AFE4 | ||||
|
nuoKE36A |
E36A |
Retains 2% dNADH oxidase and dNADH- DB reductase activity |
seeded from UniProt:P0AFE4 | ||||
|
nuoKE36Q |
E36Q |
Retains 2% dNADH oxidase and dNADH- DB reductase activity, neither a membrane potential nor a proton gradient can be formed by isolated membranes |
seeded from UniProt:P0AFE4 | ||||
|
nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire b-Methyl-D-glucoside |
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nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-D-Glucose-6-phosphate |
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nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire D-Galacturonate |
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|
nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
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nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Hydroxybutyrate |
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nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketoglutarate |
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|
nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire Succinate |
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|
nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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|
nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire Glucuronamide |
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|
nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
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nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanyl-glycine |
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|
nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Asparagine |
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|
nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Aspartate |
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|
nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire Glycyl-L-aspartate |
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|
nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Serine |
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|
nuoK(del)::kan |
deletion |
Biolog:respiration |
unable to respire Uridine |
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nuoK761(del)::kan |
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nuoK761(del)::FRT |
deletion |
Mutagenesis rate. |
Decreased Stress Induced Mutagenesis (SIM). |
Parent Strain: SMR4562 Experimental Strain: SMR13653 |
The mutation's effect on SIM was of Medium strength with a reduction of mutant frequency of 68 to 89 percent. See table S3 for full experimental data. | ||
|
nuoK761(del)::FRT |
deletion |
SDS-EDTA Sensitivity |
Parent Strain: SMR4562 Experimental Strain: SMR13653 |
The mutation conferred an increase in SDS-EDTA sensitivity. See table S7 and S1 for a summary of experimental data. | |||
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SMR4562 yiaG-yfp FRTcatFRT nuoK761(del)::FRTKanFRT |
deletion |
SigmaS activity |
Decrease in SigmaS activity |
Parental Strain: SMR10582 Experimental Strain: SMR14285 |
See table S8 for experimental data. | ||
|
CAG45114 nuoK761(del)::FRTKanFRT |
deletion |
SigmaE activity |
Decrease in sigmaE activity |
Parental Strain: CAG45115 Experimental Strain: SMR15366 |
See table S11 for full experimental data. | ||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2274 |
Plasmid clone |
Status:Clone OK Primer 1:GCCATCCCCTTACAACATGGACT Primer 2:CCTCCGCGCATCTCACTTACTGA | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 89% [8] | ||
|
zfd-1::Tn10 |
Linked marker |
est. P1 cotransduction: % [8] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 4.14 4.15 4.16 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ 5.0 5.1 5.2 5.3 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
