nuoH:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
nuoH |
|---|---|
| Mnemonic |
NADH:ubiquinone oxidoreductase |
| Synonyms |
ECK2276, b2282, JW2277[1], JW2277 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
51.61 minutes |
MG1655: 2395464..2394487 |
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NC_012967: 2341033..2340056 |
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NC_012759: 2280292..2281269 |
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W3110 |
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W3110: 2402112..2401135 |
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DH10B: 2487229..2486252 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
nuoH(del) (Keio:JW2277) |
deletion |
deletion |
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nuoH::Tn5KAN-2 (FB20740) |
Insertion at nt 323 in Plus orientation |
does not contain pKD46 | |||||
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nuoH::Tn5KAN-2 (FB20741) |
Insertion at nt 323 in Plus orientation |
contains pKD46 | |||||
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire Dextrin |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire D-Galactose |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire D-Mannitol |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire b-Methyl-D-glucoside |
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|
nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-D-Glucose |
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|
nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-D-Glucose-1-phosphate |
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|
nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-D-Glucose-6-phosphate |
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|
nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire DL-a-Glycerol |
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|
nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Hydroxybutyrate |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketoglutarate |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire DL-Lactate |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire Succinate |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire Glucuronamide |
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|
nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanyl-glycine |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Asparagine |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Aspartate |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire Glycyl-L-aspartate |
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|
nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Serine |
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nuoH(del)::kan |
deletion |
Biolog:respiration |
unable to respire Uridine |
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nuoH764(del)::kan |
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nuoH764(del)::FRT |
deletion |
Mutagenesis Rate |
Decrease in Stress Induced Mutagenesis (SIM). |
Parent Strain: SMR4562 Wild Type Strain: SMR13654 |
The mutation caused a strong reduction in SIM with a decrease of mutant frequency by over 90 % of the wild type. See figure S3 for experimental results. | ||
|
nuoH764(del)::FRT |
deletion |
Sensitivity to |
SDS-EDTA Sensitivity |
Parent Strain: SMR4562 Wild Type Strain: SMR13654 |
The mutation caused an increase in SDS-EDTA Sensitivity. See table S7 and S1 for a summary of experimental results. | ||
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SMR4562 yiaG-yfp FRTcatFRT nuoH764(del)::FRTKanFR |
deletion |
Sigma S activity |
Decrease in SigmaS activity. |
Parental Strain: SMR10582 Experimental Strain: SMR14287 |
See table S8 for full experimental data. | ||
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CAG45114 nuoH764::FRTKanFRT |
Insertion |
SigmaE activity |
Decrease in sigmaE activity |
Parental Strain: CAG45114 Experimental Strain: SMR15249 |
See table S11 for full experimental data. | ||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2277 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAGTTGGATATCACCGGAACT Primer 2:CCTTGCGCCTGCCAGAGAATGAC | |
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Kohara Phage |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 85% [9] | ||
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zfd-1::Tn10 |
Linked marker |
est. P1 cotransduction: % [9] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 5.12 5.13 5.14 5.15 5.16 5.17 5.18 5.19 5.20 5.21 5.22 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ 6.0 6.1 6.2 6.3 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 8.0 8.1 8.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
