nuoC:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
nuoC |
---|---|
Gene Synonym(s) |
ECK2280, b2286, JW5375, nuoCD, nuoD[1], nuoD |
Product Desc. |
Component of connecting fragment of NADH dehydrogenase I[3]; NADH dehydrogenase I[2][3] NADH:ubiquinone oxidoreductase subunit C, complex I; NADH dehydrogenase I[4] |
Product Synonyms(s) |
NADH:ubiquinone oxidoreductase, chain C,D[1], B2286[2][1], NuoD[2][1], NuoCD[2][1], NuoC[2][1] , ECK2280, JW5375, nuoCD, nuoD, b2286 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression |
transcription unit(s): nuoABCEFGHIJKLMN[2], nuo1-14 |
Regulation/Activity | |
Quick Links | |
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Notes
Leif (1995) find the starting amino acids at codon +4 (MVNN), in conflict with the more recent data of David (2002), which does not include these 4 amino acids and the initial Met. Alignment with Yersinia NuoC supports the second ATG annotated here. The reason for the discrepancy is not known as both used native protein preparations, although the protein band sequenced by Leif et al. was not the correct MW for the revised, fused NuoCD protein sequence. HT_Cmplx18_Mem: NuoB+NuoC.[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
nuoC |
---|---|
Mnemonic |
NADH:ubiquinone oxidoreductase |
Synonyms |
ECK2280, b2286, JW5375, nuoCD, nuoD[1], nuoD |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
51.73 minutes |
MG1655: 2401879..2400077 |
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NC_012967: 2347448..2345646 |
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NC_012759: 2285882..2287672 |
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W3110 |
|
W3110: 2408527..2406725 |
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DH10B: 2493644..2491842 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
Coding Start (SO:0000323) |
2400080 |
Edman degradation |
PMID:7607227 |
| |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
nuoC(del) (Keio:JW5375) |
deletion |
deletion |
PMID:16738554 |
||||
nuoC768(del)::FRT |
deletion |
Mutagenesis rate |
Decrease in rate of stress induced mutagenesis (SIM) |
PMID:23224554 |
Parent Strain: SMR4162 Experimental Strain: SMR12223 |
The mutation conferred a strong decrease in the rate of SIM, the mutation frequency being roughly 90 percent less than the wild type. See table S3 for full experimental results. | |
nuoC768(del)::FRT |
deletion |
Sensitivity to |
Increased SDS-EDTA sensitivity |
PMID:23224554 |
Parent Strain: SMR4162 Experimental Strain: SMR12223 |
The mutation conferred an increase in SDS-EDTA sensitivity relative to the wild type. See tables S7 and S1 for a summary of experimental data. | |
SMR4562 nuoC768(del)::FRT yiaG-yfp FRTcatFRT |
Deletion |
Sigma S |
Decrease in SigmaS activity |
PMID:23224554 |
Parental Strain: SMR10582 Experimental Strain: SMR12655 |
See table S8 for full experimental results. | |
CAG45114 nuoC768(del)::RTKanFRT |
deltion |
SigmaE activity |
Decrease in sigmaE activity |
PMID:23224554 |
Parental Strain: CAG45115 Experimental Strain: SMR15277 |
See table S11 for full experimental data. | |
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW5375 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCGTGAACAATATGACCGACTT Primer 2:CCGCGGTCCACATCTGACATAAC | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 69% [6] | ||
zfd-1::Tn10 |
Linked marker |
est. P1 cotransduction: % [6] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG12084 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12084 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001990 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB2009 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0007549 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
NuoC |
---|---|
Synonyms |
NADH:ubiquinone oxidoreductase, chain C,D[1], B2286[2][1], NuoD[2][1], NuoCD[2][1], NuoC[2][1] , ECK2280, JW5375, nuoCD, nuoD, b2286 |
Product description |
Component of connecting fragment of NADH dehydrogenase I[3]; NADH dehydrogenase I[2][3] NADH:ubiquinone oxidoreductase subunit C, complex I; NADH dehydrogenase I[4] |
EC number (for enzymes) | |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
NOT |
GO:0005737 |
cytoplasm |
PMID:7607227 |
IDA: Inferred from Direct Assay |
C |
Seeded from Riley et al 2006 [1]. See discussion page for explanation of 'NOT' qualifier. |
complete | |
GO:0003824 |
catalytic activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0511 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005886 |
plasma membrane |
PMID:7607227 |
IDA: Inferred from Direct Assay |
C |
The NADH dehydrogenase I complex fractionates with cytoplasmic membranes. |
complete | ||
GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0963 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
Contributes to |
GO:0003954 |
NADH dehydrogenase activity |
PMID:3122832 |
IDA: Inferred from Direct Assay |
F |
Purified NADH dehydrogenase I in membrane vesicles can oxidize NADH. |
complete | |
GO:0005737 |
cytoplasm |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006810 |
transport |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0813 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0030964 |
NADH dehydrogenase complex |
PMID:7607227 |
IDA: Inferred from Direct Assay |
C |
The E. coli NADH dehydrogenase complex was purified and the subunits separated by SDS-PAGE. The N-termini of these 6 polypeptides were sequenced after deblocking with methanolic HCl. |
complete | ||
GO:0008137 |
NADH dehydrogenase (ubiquinone) activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001268 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016020 |
membrane |
PMID:16858726 |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0016491 |
oxidoreductase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0560 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016651 |
oxidoreductase activity, acting on NADH or NADPH |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001135 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016651 |
oxidoreductase activity, acting on NADH or NADPH |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010218 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016651 |
oxidoreductase activity, acting on NADH or NADPH |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010219 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0019898 |
extrinsic to membrane |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-9903 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0048038 |
quinone binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001135 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0048038 |
quinone binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0874 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0050136 |
NADH dehydrogenase (quinone) activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_01359 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0050136 |
NADH dehydrogenase (quinone) activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
EC:1.6.99.5 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0051287 |
NAD or NADH binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001135 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001135 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001268 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010218 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010219 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0560 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of connecting fragment of NADH dehydrogenase I |
could be indirect |
||
Protein |
gadB |
PMID:19402753 |
MALDI(Z-score):19.246482 | |
Protein |
pcnB |
PMID:19402753 |
MALDI(Z-score):24.739832 | |
Protein |
pflB |
PMID:19402753 |
MALDI(Z-score):20.070712 | |
Protein |
dps |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
metK |
PMID:19402753 |
MALDI(Z-score):20.921273 | |
Protein |
cysJ |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
cysI |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
crr |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
ycbX |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
Subunits of NADH dehydrogenase I |
could be indirect |
| |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
Cytoplasm |
PMID:7607227 |
| ||
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MVNNMTDLTA QEPAWQTRDH LDDPVIGELR NRFGPDAFTV QATRTGVPVV WIKREQLLEV GDFLKKLPKP YVMLFDLHGM DERLRTHREG LPAADFSVFY HLISIDRNRD IMLKVALAEN DLHVPTFTKL FPNANWYERE TWDLFGITFD GHPNLRRIMM PQTWKGHPLR KDYPARATEF SPFELTKAKQ DLEMEALTFK PEEWGMKRGT ENEDFMFLNL GPNHPSAHGA FRIVLQLDGE EIVDCVPDIG YHHRGAEKMG ERQSWHSYIP YTDRIEYLGG CVNEMPYVLA VEKLAGITVP DRVNVIRVML SELFRINSHL LYISTFIQDV GAMTPVFFAF TDRQKIYDLV EAITGFRMHP AWFRIGGVAH DLPRGWDRLL REFLDWMPKR LASYEKAALQ NTILKGRSQG VAAYGAKEAL EWGTTGAGLR ATGIDFDVRK ARPYSGYENF DFEIPVGGGV SDCYTRVMLK VEELRQSLRI LEQCLNNMPE GPFKADHPLT TPPPKERTLQ HIETLITHFL QVSWGPVMPA NESFQMIEAT KGINSYYLTS DGSTMSYRTR VRTPSFAHLQ QIPAAIRGSL VSDLIVYLGS IDFVMSDVDR |
Length |
600 |
Mol. Wt |
68.695 kDa |
pI |
6.4 (calculated) |
Extinction coefficient |
95,800 - 96,300 (calc based on 20 Y, 12 W, and 4 C residues) |
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Domains/Motifs/Modification Sites
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<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0007549 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG12084 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12084 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120001990 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB2009 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
Ecoli K-12 |
32.453+/-0.16 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.216981132 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
Protein |
E. coli K-12 MG1655 |
3010 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
1164 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
2039 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:2401859..2401899
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
</protect>
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
<protect>
Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for nuoC | |
microarray |
Summary of data for nuoC from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to nuoC Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG12084 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB2009 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12084 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001990 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0007549 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Anopheles gambiae |
|
From Inparanoid:20070104 |
Apis mellifera |
|
From Inparanoid:20070104 |
Bos taurus |
|
From Inparanoid:20070104 |
Caenorhabditis briggsae |
|
From Inparanoid:20070104 |
Caenorhabditis elegans |
|
From Inparanoid:20070104 |
Canis familiaris |
|
From Inparanoid:20070104 |
Ciona intestinalis |
|
From Inparanoid:20070104 |
Danio rerio |
|
From Inparanoid:20070104 |
Dictyostelium discoideum |
|
From Inparanoid:20070104 |
Drosophila melanogaster |
|
From Inparanoid:20070104 |
Drosophila pseudoobscura |
|
From Inparanoid:20070104 |
Gallus gallus |
|
From Inparanoid:20070104 |
Homo sapiens |
|
From Inparanoid:20070104 |
Macaca mulatta |
|
From Inparanoid:20070104 |
Monodelphis domestica |
|
From Inparanoid:20070104 |
Mus musculus |
|
From Inparanoid:20070104 |
Oryza gramene |
|
From Inparanoid:20070104 |
Pan troglodytes |
|
From Inparanoid:20070104 |
Takifugu rubripes |
|
From Inparanoid:20070104 |
Tetraodon nigroviridis |
|
From Inparanoid:20070104 |
Xenopus tropicalis |
|
From Inparanoid:20070104 |
Shigella flexneri |
NUOC |
From SHIGELLACYC |
E. coli O157 |
NUOC |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG12084 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12084 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001990 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB2009 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0007549 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
Categories
- Genes with homologs in Anopheles gambiae
- Genes with homologs in Apis mellifera
- Genes with homologs in Bos taurus
- Genes with homologs in Caenorhabditis briggsae
- Genes with homologs in Caenorhabditis elegans
- Genes with homologs in Canis familiaris
- Genes with homologs in Ciona intestinalis
- Genes with homologs in Danio rerio
- Genes with homologs in Dictyostelium discoideum
- Genes with homologs in Drosophila melanogaster
- Genes with homologs in Drosophila pseudoobscura
- Genes with homologs in Gallus gallus
- Genes with homologs in Homo sapiens
- Genes with homologs in Macaca mulatta
- Genes with homologs in Monodelphis domestica
- Genes with homologs in Mus musculus
- Genes with homologs in Oryza gramene
- Genes with homologs in Pan troglodytes
- Genes with homologs in Takifugu rubripes
- Genes with homologs in Tetraodon nigroviridis
- Genes with homologs in Xenopus tropicalis
- Genes with homologs in Shigella flexneri
- Genes with homologs in E. coli O157