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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	nuoA
Gene Synonym(s)	ECK2282, b2288, JW2283^[1], JW2283
Product Desc.	NuoA^[2]^[3]; Component of NADH dehydrogenase I^[2]^[3] NADH:ubiquinone oxidoreductase subunit A, complex I; NADH dehydrogenase I^[4]
Product Synonyms(s)	NADH:ubiquinone oxidoreductase, membrane subunit A^[1], B2288^[2]^[1], NuoA^[2]^[1] , ECK2282, JW2283, b2288
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): nuoABCEFGHIJKLMN^[2], nuo1-14
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	nuoA
Mnemonic	NADH:ubiquinone oxidoreductase
Synonyms	ECK2282, b2288, JW2283^[1], JW2283
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	51.78 minutes, 51.78 minutes	MG1655: 2403094..2402651 <gbrowseImage> name=NC_000913:2402651..2403094 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2348663..2348220 <gbrowseImage> name=NC_012967:2348220..2348663 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2288456..2288899 <gbrowseImage> name=NC_012759:2288456..2288899 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2409742..2409299 <gbrowseImage> name=NC_007779:2409299..2409742 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2494859..2494416 <gbrowseImage> name=NC_010473:2494416..2494859 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔnuoA (Keio:JW2283)	deletion	deletion			PMID:16738554	Shigen CGSC8305^[5]
nuoA::Tn5KAN-2 (FB20749)	Insertion at nt 293 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20749	does not contain pKD46
nuoA::Tn5KAN-2 (FB20750)	Insertion at nt 293 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20750	contains pKD46
ΔnuoA::kan	deletion		Biolog:respiration	unable to respire b-Methyl-D-glucoside	PMID:16095938
ΔnuoA::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
ΔnuoA::kan	deletion		Biolog:respiration	unable to respire a-Ketobutyrate	PMID:16095938
ΔnuoA::kan	deletion		Biolog:respiration	unable to respire a-Ketoglutarate	PMID:16095938
ΔnuoA::kan	deletion		Biolog:respiration	unable to respire Succinate	PMID:16095938
ΔnuoA::kan	deletion		Biolog:respiration	unable to respire Bromosuccinate	PMID:16095938
ΔnuoA::kan	deletion		Biolog:respiration	unable to respire Glucuronamide	PMID:16095938
ΔnuoA::kan	deletion		Biolog:respiration	unable to respire L-Alanine	PMID:16095938
ΔnuoA::kan	deletion		Biolog:respiration	unable to respire L-Alanyl-glycine	PMID:16095938
ΔnuoA::kan	deletion		Biolog:respiration	unable to respire L-Asparagine	PMID:16095938
ΔnuoA::kan	deletion		Biolog:respiration	unable to respire L-Aspartate	PMID:16095938
ΔnuoA::kan	deletion		Biolog:respiration	unable to respire Glycyl-L-aspartate	PMID:16095938
ΔnuoA::kan	deletion		Biolog:respiration	unable to respire L-Serine	PMID:16095938
ΔnuoA770::kan					PMID:16738554	CGSC:99824
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Notes

The nuo region was identified^[6] initially from Hfr matings into strain IY12, which carried mutations in both the nuo and ndh"" loci.

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2283	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGTATGTCAACATCCACTGA Primer 2:CCGCGTTGACGATTAGCGATACT
9C2	Kohara Phage	Genobase	PMID:3038334
zfb-223::Tn10	Linked marker	CAG18484 = CGSC7406^[5]	est. P1 cotransduction: 63% ^[7] Synonyms:zej-223::Tn10
zfd-1::Tn10	Linked marker	CAG18467 = CGSC7408^[5]	est. P1 cotransduction: % ^[7] Synonyms:zfb-1::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12082	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12082	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001988	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946764	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2007	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007553	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	NuoA
Synonyms	NADH:ubiquinone oxidoreductase, membrane subunit A^[1], B2288^[2]^[1], NuoA^[2]^[1] , ECK2282, JW2283, b2288
Product description	NuoA^[2]^[3]; Component of NADH dehydrogenase I^[2]^[3] NADH:ubiquinone oxidoreductase subunit A, complex I; NADH dehydrogenase I^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008137	NADH dehydrogenase (ubiquinone) activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000440	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	PMID:7607227	IDA: Inferred from Direct Assay		C	The NADH dehydrogenase I complex fractionates with cytoplasmic membranes.	complete
Contributes to	GO:0003954	NADH dehydrogenase activity	PMID:3122832	IDA: Inferred from Direct Assay		F	Purified NADH dehydrogenase I in membrane vesicles can oxidize NADH.	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0030964	NADH dehydrogenase complex	PMID:7607227	IDA: Inferred from Direct Assay		C	E. coli NADH dehydrogenase I was purified and the subunits separated by SDS-PAGE. These seven subunits were not recovered from the gel in sufficient amounts to allow Edman degradation. The polypeptides were assigned to their respective genes based on their apparent molecular masses.	complete
	GO:0019684	photosynthesis, light reaction	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01394	P	Seeded from EcoCyc (v14.0)	complete
	GO:0048038	quinone binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0874	F	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01394	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000440	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of NADH dehydrogenase I	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSMSTSTEVI AHHWAFAIFL IVAIGLCCLM LVGGWFLGGR ARARSKNVPF ESGIDSVGSA RLRLSAKFYL VAMFFVIFDV EALYLFAWST SIRESGWVGF VEAAIFIFVL LAGLVYLVRI GALDWTPARS RRERMNPETN SIANRQR
Length	147
Mol. Wt	16.457 kDa
pI	10.2 (calculated)
Extinction coefficient	31,970 - 32,220 (calc based on 3 Y, 5 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	25..125	PF00507 NADH-ubiquinone/plastoquinone oxidoreductase, chain 3		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nuoA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:49176207	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946764	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007553	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AFC3	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12082	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12082	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946764	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001988	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2007	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.04E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	109.877+/-0.444	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.16291+/-0.01511	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by FISH	PMID:20671182
mRNA	Ecoli K-12	0.261851016	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	3084	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1083	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1022	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
nuoABCEFGHIJKLMN
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2403074..2403114 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2288 (EcoliWiki Page)	NCBI GEO profiles for nuoA
microarray	GenExpDB:b2288 (EcoliWiki Page)	Summary of data for nuoA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to nuoA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12082	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2007	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2288
EcoGene	EcoGene:EG12082	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001988	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007553	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT2G07751 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-011205-9 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0201594 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000028434 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000355206 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:102499 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q6ENV8 (score: 1.000; bootstrap: 100%) P19044 (score: 1.000; bootstrap: 100%) Q6L394 (score: 1.000; bootstrap: 100%) Q7G225 (score: 0.952) Q6QY70 (score: 0.952) P12126 (score: 0.952) Q6ENG9 (score: 0.952) P26303 (score: 0.934) Q85XC4 (score: 0.922) Q8HCQ0 (score: 0.108) P60160 (score: 0.102) Q6R9F4 (score: 0.102) Q9G9Y1 (score: 0.096) P16265 (score: 0.096)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000039725 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000049319 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	NUOA	From SHIGELLACYC
E. coli O157	NUOA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00507 NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
Panther (EcoliWiki Page)	PTHR11058:SF4
EcoCyc	EcoCyc:EG12082	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12082	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001988	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2007	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007553	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ Calhoun, MW & Gennis, RB (1993) Demonstration of separate genetic loci encoding distinct membrane-bound respiratory NADH dehydrogenases in Escherichia coli. J. Bacteriol. 175 3013-9 PubMed
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

nuoA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to nuoA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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