nrdA:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
nrdA |
---|---|
Gene Synonym(s) |
ECK2226, b2234, JW2228, dnaF[1], dnaF |
Product Desc. |
Component of B1 protein[2][3]; ribonucleotide reductase I[2] Ribonucleoside diphosphate reductase, subunit alpha; class I, aerobic; ribonucleotide reductase; B1 protein, R1 subunit[4] |
Product Synonyms(s) |
ribonucleoside diphosphate reductase 1, alpha subunit[1], B2234[2][1], DnaF[2][1], NrdA[2][1] , dnaF, ECK2226, JW2228, b2234 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
Reducing equivalents provided by glutaredoxin or thioredoxin. Binds TrxA (Kumar, 2004). About half of the overproduced protein has the first one or two amino acids removed. E. coli B native protein is reported to start with Gln. a second native E. coli B protein start may correspond to a cleavage after Leu26. Leu26 CTG codon is unlikely to be an alternative start codon in K-12 or B and no evidence of this cleavage or an alternative start codon was seen with the overproduced K-12 protein. An M-N or MN-Q cleavage by MAP would be unusual. The variable N-termini may be created by an unknown protease.[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
nrdA |
---|---|
Mnemonic |
Nucleotide reductase |
Synonyms |
ECK2226, b2234, JW2228, dnaF[1], dnaF |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
50.5 minutes |
MG1655: 2342887..2345172 |
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NC_012967: 2291565..2293850 |
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NC_012759: 2228692..2230977 |
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W3110 |
|
W3110: 2349535..2351820 |
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DH10B: 2433875..2436160 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
Coding Start (SO:0000323) |
2342887 |
Edman degradation |
PMID:3894026 |
| |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
nrdA |
Deletion | ||||||
nrdA |
Deletion | ||||||
nrdAE441A,Q |
E441A,Q |
Loss of activity |
seeded from UniProt:P00452 | ||||
nrdAE441D |
E441D |
Decrease in activity |
seeded from UniProt:P00452 | ||||
nrdAY730F |
Y730F |
Loss of activity |
seeded from UniProt:P00452 | ||||
nrdAY731F |
Y731F |
Loss of activity |
seeded from UniProt:P00452 | ||||
nrdA1(ts) |
temperature sensitive |
||||||
nrdA pPS1 |
Resistant to |
increased resistance to hydroxyurea |
PMID:7009552 |
KK535 |
figure 4B. | ||
nrdA pPS2 |
Resistant to |
resistance to hydroxyurea |
PMID:7009552 |
KK535 |
figure 4B | ||
nrdA pPS101 |
Resistant to |
slight increase in hydroxyurea resistance |
PMID:7009552 |
KK535 |
figure 4 | ||
nrdA pPS201 |
Resistant to |
slight increase resistance to hydroxyurea |
PMID:7009552 |
KK535 |
figure 4 | ||
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW2228 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCAATCAGAATCTGCTGGTGAC Primer 2:CCGATCTTACATGCGCCGCTTTC | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 42% [6] | ||
Linked marker |
est. P1 cotransduction: 100% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10660 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10660 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000653 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB0654 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0007383 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
NrdA |
---|---|
Synonyms |
ribonucleoside diphosphate reductase 1, alpha subunit[1], B2234[2][1], DnaF[2][1], NrdA[2][1] , dnaF, ECK2226, JW2228, b2234 |
Product description |
Component of B1 protein[2][3]; ribonucleotide reductase I[2] Ribonucleoside diphosphate reductase, subunit alpha; class I, aerobic; ribonucleotide reductase; B1 protein, R1 subunit[4] |
EC number (for enzymes) | |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0015949 |
nucleobase, nucleoside and nucleotide interconversion |
PMID:13973714 |
IDA: Inferred from Direct Assay |
P |
complete | |||
GO:0005971 |
ribonucleoside-diphosphate reductase complex |
PMID:13973714 |
IDA: Inferred from Direct Assay |
C |
complete | |||
GO:0009263 |
deoxyribonucleotide biosynthetic process |
PMID:13973714 |
IDA: Inferred from Direct Assay |
P |
complete | |||
GO:0004748 |
ribonucleoside-diphosphate reductase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000788 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004748 |
ribonucleoside-diphosphate reductase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008926 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
PMID:4902211 |
IDA: Inferred from Direct Assay |
F |
complete | |||
GO:0004748 |
ribonucleoside-diphosphate reductase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR013509 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
Contributes to |
GO:0004748 |
ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor |
PMID:13973714 |
IDA: Inferred from Direct Assay |
F |
replaces outdated GO:0016959 |
complete | |
GO:0004748 |
ribonucleoside-diphosphate reductase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
EC:1.17.4.1 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005515 |
protein binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008926 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR013509 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0067 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005971 |
ribonucleoside-diphosphate reductase complex |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000788 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005971 |
ribonucleoside-diphosphate reductase complex |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008926 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005971 |
ribonucleoside-diphosphate reductase complex |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR013346 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006260 |
DNA replication |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000788 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006260 |
DNA replication |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008926 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006260 |
DNA replication |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR013509 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006260 |
DNA replication |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0235 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008926 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR013346 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR013509 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0560 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of B1 protein |
could be indirect |
||
Protein |
mreB |
PMID:15690043 |
Experiment(s):EBI-881495 | |
Protein |
proA |
PMID:15690043 |
Experiment(s):EBI-881495 | |
Protein |
tufA |
PMID:15690043 |
Experiment(s):EBI-881495 | |
Protein |
bglX |
PMID:16606699 |
Experiment(s):EBI-1142281 | |
Protein |
mreB |
PMID:19402753 |
MALDI(Z-score):35.698224 | |
Protein |
Subunits of ribonucleotide reductase I |
could be indirect |
| |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MNQNLLVTKR DGSTERINLD KIHRVLDWAA EGLHNVSISQ VELRSHIQFY DGIKTSDIHE TIIKAAADLI SRDAPDYQYL AARLAIFHLR KKAYGQFEPP ALYDHVVKMV EMGKYDNHLL EDYTEEEFKQ MDTFIDHDRD MTFSYAAVKQ LEGKYLVQNR VTGEIYESAQ FLYILVAACL FSNYPRETRL QYVKRFYDAV STFKISLPTP IMSGVRTPTR QFSSCVLIEC GDSLDSINAT SSAIVKYVSQ RAGIGINAGR IRALGSPIRG GEAFHTGCIP FYKHFQTAVK SCSQGGVRGG AATLFYPMWH LEVESLLVLK NNRGVEGNRV RHMDYGVQIN KLMYTRLLKG EDITLFSPSD VPGLYDAFFA DQEEFERLYT KYEKDDSIRK QRVKAVELFS LMMQERASTG RIYIQNVDHC NTHSPFDPAI APVRQSNLCL EIALPTKPLN DVNDENGEIA LCTLSAFNLG AINNLDELEE LAILAVRALD ALLDYQDYPI PAAKRGAMGR RTLGIGVINF AYYLAKHGKR YSDGSANNLT HKTFEAIQYY LLKASNELAK EQGACPWFNE TTYAKGILPI DTYKKDLDTI ANEPLHYDWE ALRESIKTHG LRNSTLSALM PSETSSQISN ATNGIEPPRG YVSIKASKDG ILRQVVPDYE HLHDAYELLW EMPGNDGYLQ LVGIMQKFID QSISANTNYD PSRFPSGKVP MQQLLKDLLT AYKFGVKTLY YQNTRDGAED AQDDLVPSIQ DDGCESGACK I |
Length |
761 |
Mol. Wt |
85.778 kDa |
pI |
5.7 (calculated) |
Extinction coefficient |
88,590 - 89,965 (calc based on 41 Y, 5 W, and 11 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0007383 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG10660 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10660 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120000653 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0654 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MC4100 |
1.08E+03 |
molecules/cell |
|
emPAI |
PMID:18304323 | |
Protein |
Ecoli K-12 |
251.1+/-1.592 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.02047+/-0.00547 |
Molecules/cell |
|
by FISH |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.061706783 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
Protein |
E. coli K-12 MG1655 |
6667 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
904 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
2748 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:2342867..2342907
source=MG1655
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for nrdA | |
microarray |
Summary of data for nrdA from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to nrdA Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10660 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0654 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10660 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000653 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0007383 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Anopheles gambiae |
|
From Inparanoid:20070104 |
Apis mellifera |
|
From Inparanoid:20070104 |
Arabidopsis thaliana |
|
From Inparanoid:20070104 |
Bos taurus |
|
From Inparanoid:20070104 |
Caenorhabditis briggsae |
|
From Inparanoid:20070104 |
Caenorhabditis elegans |
|
From Inparanoid:20070104 |
Canis familiaris |
|
From Inparanoid:20070104 |
Ciona intestinalis |
|
From Inparanoid:20070104 |
Danio rerio |
|
From Inparanoid:20070104 |
Dictyostelium discoideum |
|
From Inparanoid:20070104 |
Drosophila melanogaster |
|
From Inparanoid:20070104 |
Gallus gallus |
|
From Inparanoid:20070104 |
Homo sapiens |
|
From Inparanoid:20070104 |
Macaca mulatta |
|
From Inparanoid:20070104 |
Monodelphis domestica |
|
From Inparanoid:20070104 |
Mus musculus |
|
From Inparanoid:20070104 |
Oryza gramene |
|
From Inparanoid:20070104 |
Pan troglodytes |
|
From Inparanoid:20070104 |
Saccharomyces cerevisiae |
|
From Inparanoid:20070104 |
Schizosaccharomyces pombe |
|
From Inparanoid:20070104 |
Takifugu rubripes |
|
From Inparanoid:20070104 |
Tetraodon nigroviridis |
|
From Inparanoid:20070104 |
Xenopus tropicalis |
|
From Inparanoid:20070104 |
Shigella flexneri |
NRDA |
From SHIGELLACYC |
E. coli O157 |
NRDA |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10660 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10660 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000653 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0654 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0007383 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
Categories
- Genes with homologs in Anopheles gambiae
- Genes with homologs in Apis mellifera
- Genes with homologs in Arabidopsis thaliana
- Genes with homologs in Bos taurus
- Genes with homologs in Caenorhabditis briggsae
- Genes with homologs in Caenorhabditis elegans
- Genes with homologs in Canis familiaris
- Genes with homologs in Ciona intestinalis
- Genes with homologs in Danio rerio
- Genes with homologs in Dictyostelium discoideum
- Genes with homologs in Drosophila melanogaster
- Genes with homologs in Gallus gallus
- Genes with homologs in Homo sapiens
- Genes with homologs in Macaca mulatta
- Genes with homologs in Monodelphis domestica
- Genes with homologs in Mus musculus
- Genes with homologs in Oryza gramene
- Genes with homologs in Pan troglodytes
- Genes with homologs in Saccharomyces cerevisiae
- Genes with homologs in Schizosaccharomyces pombe
- Genes with homologs in Takifugu rubripes
- Genes with homologs in Tetraodon nigroviridis
- Genes with homologs in Xenopus tropicalis
- Genes with homologs in Shigella flexneri
- Genes with homologs in E. coli O157