nrdA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
nrdA |
|---|---|
| Mnemonic |
Nucleotide reductase |
| Synonyms |
ECK2226, b2234, JW2228, dnaF[1], dnaF |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
50.5 minutes |
MG1655: 2342887..2345172 |
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NC_012967: 2291565..2293850 |
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NC_012759: 2228692..2230977 |
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W3110 |
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W3110: 2349535..2351820 |
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DH10B: 2433875..2436160 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2342887 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
nrdA |
Deletion | ||||||
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nrdA |
Deletion | ||||||
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nrdAE441A,Q |
E441A,Q |
Loss of activity |
seeded from UniProt:P00452 | ||||
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nrdAE441D |
E441D |
Decrease in activity |
seeded from UniProt:P00452 | ||||
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nrdAY730F |
Y730F |
Loss of activity |
seeded from UniProt:P00452 | ||||
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nrdAY731F |
Y731F |
Loss of activity |
seeded from UniProt:P00452 | ||||
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nrdA1(ts) |
temperature sensitive |
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nrdA pPS1 |
Resistant to |
increased resistance to hydroxyurea |
KK535 |
figure 4B. | |||
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nrdA pPS2 |
Resistant to |
resistance to hydroxyurea |
KK535 |
figure 4B | |||
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nrdA pPS101 |
Resistant to |
slight increase in hydroxyurea resistance |
KK535 |
figure 4 | |||
|
nrdA pPS201 |
Resistant to |
slight increase resistance to hydroxyurea |
KK535 |
figure 4 | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2228 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAATCAGAATCTGCTGGTGAC Primer 2:CCGATCTTACATGCGCCGCTTTC | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 42% [7] | ||
|
Linked marker |
est. P1 cotransduction: 100% [7] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Sjöberg, BM et al. (1985) Protein B1 of ribonucleotide reductase. Direct analytical data and comparisons with data indirectly deduced from the nucleotide sequence of the Escherichia coli nrdA gene. Eur. J. Biochem. 150 423-7 PubMed
- ↑ 3.0 3.1 3.2 3.3 Platz, A & Sjöberg, BM (1980) Construction and characterization of hybrid plasmids containing the Escherichia coli nrd region. J. Bacteriol. 143 561-8 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
- ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
