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Standard Name	nhaA
Gene Synonym(s)	ECK0020, b0019, JW0018, ant, antA, hyc^[1]
Product Desc.	sodium/proton NhaA transporter^[2]^[3] Na+/H+ antiporter 1, strongly pH-dependent; helps regulate intracellular pH and extrude lithium; nhaA_P1 activated by NhaR, repressed by H-NS and stimulated by Na(+)^[4]
Product Synonyms(s)	sodium-proton antiporter^[1], B0019^[2]^[1], Hyc^[2]^[1], Ant^[2]^[1], AntA^[2]^[1], NhaA^[2]^[1] , ant, antA, ECK0020, JW0018, b0019
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): nhaA^[2], OP00281
Regulation/Activity
Quick Links
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Notes

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nhaA P2 promoter is RpoS-regulated, but H-NS, Na(+)- and NhaR-independent.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	nhaA
Mnemonic	Na+/H+ antiporter
Synonyms	ECK0020, b0019, JW0018, ant, antA, hyc^[1]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	0.38 minutes	MG1655: 17489..18655 <gbrowseImage> name=NC_000913:17489..18655 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 17488..18654 <gbrowseImage> name=NC_012967:17488..18654 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 17489..18655 <gbrowseImage> name=NC_012759:17489..18655 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 17489..18655 <gbrowseImage> name=NC_007779:17489..18655 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 17489..18655 <gbrowseImage> name=NC_010473:17489..18655 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	17489	Edman degradation	PMID:8381959
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
nhaA(del) (Keio:JW0018)	deletion	deletion			PMID:16738554	Shigen CGSC8344^[5]
nhaAD163C		D163C		Loss of antiport activity			seeded from UniProt:P13738
nhaAD163N		D163N		Loss of antiport activity. Abolishes ability to grow on high salt medium			seeded from UniProt:P13738
nhaAD164N		D164N		Loss of antiport activity. Abolishes ability to grow on high salt medium			seeded from UniProt:P13738
nhaAH225A		H225A		Loss of antiport activity. Abolishes ability to grow at alkaline pH			seeded from UniProt:P13738
nhaAH225C,S		H225C,S		Slightly reduced antiport activity. No effect on pH sensitivity			seeded from UniProt:P13738
nhaAG338S		G338S		Loss of pH-sensitivity			seeded from UniProt:P13738
nhaAH225D,R		H225D,R		Shifts threshold for pH-sensitive inactivation			seeded from UniProt:P13738
nhaAD133N		D133N		Loss of antiport activity. Abolishes ability to grow on high salt medium			seeded from UniProt:P13738
nhaA1(del)::kan					PMID:2555351 PMID:8093613	CGSC:78967
nhaA737(del)::kan					PMID:16738554	CGSC:102060
nhaA(del) nhaR(del)			Sensitivity to	Sensitive to Li⁺	PMID:1316901	Strain: OR200
nhaA(del)			Sensitivity to	Sensitivity to Li⁺	PMID:1316901		Table 4A
nhaA multicopy			Resistant to	Resistance to Li ⁺	PMID:1316901	Strains: OR100/pKR225 and OR200/pKR225
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0018	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAACATCTGCATCGATTCTT Primer 2:CCAACTGATGGACGCAAACGAAC
9E4	Kohara Phage	Genobase	PMID:3038334
6H3	Kohara Phage	Genobase	PMID:3038334
22B12	Kohara Phage	Genobase	PMID:3038334
thrA34::Tn10	Linked marker	CAG18442 = CGSC7320^[5]	est. P1 cotransduction: 53% ^[6] Synonyms:thr-34::Tn10
carB96::Tn10	Linked marker	CAG12093 = CGSC7322^[5]	est. P1 cotransduction: 59% ^[6] Synonyms:car-96::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10652	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10652	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944758	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000645	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0646	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000068	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	NhaA
Synonyms	sodium-proton antiporter^[1], B0019^[2]^[1], Hyc^[2]^[1], Ant^[2]^[1], AntA^[2]^[1], NhaA^[2]^[1] , ant, antA, ECK0020, JW0018, b0019
Product description	sodium/proton NhaA transporter^[2]^[3] Na+/H+ antiporter 1, strongly pH-dependent; helps regulate intracellular pH and extrude lithium; nhaA_P1 activated by NhaR, repressed by H-NS and stimulated by Na(+)^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01844	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006814	sodium ion transport	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01844	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006814	sodium ion transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004670	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006814	sodium ion transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0739	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006885	regulation of pH	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004670	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015081	sodium ion transmembrane transporter activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01844	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004670	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0031402	sodium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0915	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 11 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKHLHRFFSS DASGGIILII AAILAMIMAN SGATSGWYHD FLETPVQLRV GSLEINKNML LWINDALMAV FFLLVGLEVK RELMQGSLAS LRQAAFPVIA AIGGMIVPAL LYLAFNYADP ITREGWAIPA ATDIAFALGV LALLGSRVPL ALKIFLMALA IIDDLGAIII IALFYTNDLS MASLGVAAVA IAVLAVLNLC GARRTGVYIL VGVVLWTAVL KSGVHATLAG VIVGFFIPLK EKHGRSPAKR LEHVLHPWVA YLILPLFAFA NAGVSLQGVT LDGLTSILPL GIIAGLLIGK PLGISLFCWL ALRLKLAHLP EGTTYQQIMV VGILCGIGFT MSIFIASLAF GSVDPELINW AKLGILVGSI SSAVIGYSWL RVRLRPSV
Length	388
Mol. Wt	41.357 kDa
pI	9.6 (calculated)
Extinction coefficient	55,920 - 56,295 (calc based on 8 Y, 8 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	4..380	PF06965 Na+/H+ antiporter 1		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nhaA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128013	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000068	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P13738	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10652	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10652	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944758	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000645	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944758	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0646	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	1046	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	131	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	190	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
nhaA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:17469..17509 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0019 (EcoliWiki Page)	NCBI GEO profiles for nhaA
microarray	GenExpDB:b0019 (EcoliWiki Page)	Summary of data for nhaA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (16862..17558) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ13; Well:D9^[8]
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Notes

Accessions Related to nhaA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10652	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0646	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0019
EcoGene	EcoGene:EG10652	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000645	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000068	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	NHAA	From SHIGELLACYC
E. coli O157	NHAA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF06965 Na+/H+ antiporter 1
EcoCyc	EcoCyc:EG10652	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10652	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000645	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0646	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000068	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

nhaA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to nhaA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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