ndk:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ndk

Mnemonic

Nucleoside diphosphate kinase

Synonyms

ECK2514, b2518, JW2502[1], JW2502

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

56.95 minutes 

MG1655: 2642886..2642455
<gbrowseImage> name=NC_000913:2642455..2642886 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2565683..2565252
<gbrowseImage> name=NC_012967:2565252..2565683 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2528260..2528691
<gbrowseImage> name=NC_012759:2528260..2528691 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2643520..2643089
<gbrowseImage> name=NC_007779:2643089..2643520 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2734651..2734220
<gbrowseImage> name=NC_010473:2734220..2734651 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2642458

Edman degradation

PMID:1323446[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δndk (Keio:JW2502)

deletion

deletion

PMID:16738554[3]

Shigen

ndk::Tn5KAN-2 (FB20840)

Insertion at nt 234 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20840

does not contain pKD46

ndk::Tn5KAN-2 (FB20841)

Insertion at nt 234 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20841

contains pKD46

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2502

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGCTATTGAACGTACTTTTTC

Primer 2:CCACGGGTGCGCGGGCACACTTC

2D5

Kohara Phage

Genobase

PMID:3038334[6]

7F8

Kohara Phage

Genobase

PMID:3038334[6]

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[7]

est. P1 cotransduction: 11% [8]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[7]

est. P1 cotransduction: 47% [8]
Synonyms:zff-208::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10650

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10650

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000643

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945611

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0644

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008291

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Ray, NB & Mathews, CK (1992) Nucleoside diphosphokinase: a functional link between intermediary metabolism and nucleic acid synthesis. Curr. Top. Cell. Regul. 33 343-57 PubMed
  3. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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