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Standard Name	narL
Gene Synonym(s)	ECK1215, b1221, JW1212, frdR, narR^[1]
Product Desc.	NarL transcriptional dual regulator^[2]^[3] Two-component regulator protein for nitrate/nitrite response; cognate sensor is NarX^[4]
Product Synonyms(s)	B1221^[2], NarR^[2], pleiotrophic regulation of anaerobic respiration: response regulator for nar, frd, dms and tor genes; (sensors, narX, narQ)^[2], FrdR^[2], nitrate/nitrite response regulator NarL^[2], unphosphorylated nitrate/nitrite response regulator NarL^[2], unmodified nitrate/nitrite response regulator NarL^[2] , ECK1215, frdR, JW1212, b1221
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): narXL^[2], OP00213, narL
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	narL
Mnemonic	Nitrate reductase, nitrate regulation
Synonyms	ECK1215, b1221, JW1212, frdR, narR^[1]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	27.47 minutes	MG1655: 1275052..1274402 <gbrowseImage> name=NC_000913:1274402..1275052 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1275979..1275329 <gbrowseImage> name=NC_012967:1275329..1275979 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1163123..1163773 <gbrowseImage> name=NC_012759:1163123..1163773 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1277406..1276756 <gbrowseImage> name=NC_007779:1276756..1277406 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1315293..1314643 <gbrowseImage> name=NC_010473:1314643..1315293 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
narL(del) (Keio:JW1212)	deletion	deletion			PMID:16738554	Shigen CGSC9101^[5]
narL(del)::kan	deletion		Biolog:respiration	unable to respire D-Mannose	PMID:16095938
narL732(del)::kan					PMID:16738554	CGSC:102026
nar-215::Tn10			Enzyme Activity	mutant is narL^- and has reduced Nitrate Reductase Activity	PMID:7047497	Experimental strain: RK5278	table 5
nar-215::Tn10			Enzyme Activity	mutant is narL ^- and has decreased formate activity	PMID:7047497	Experimental Strain: RK5278	Table 5
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Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1212	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGTAATCAGGAACCGGCTAC Primer 2:CCGAAAATGCGCTCCTGATGCAC
13H6	Kohara Phage	Genobase	PMID:3038334
fadR13::Tn10	Linked marker	CAG18497 = CGSC7365^[5]	est. P1 cotransduction: 18% ^[6]
oppC506::Tn10	Linked marker	CAG12169 = CGSC7369^[5]	est. P1 cotransduction: 32% ^[6] Synonyms:zch-506::Tn10, zci-506::Tn10
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc	EcoCyc:EG10643	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10643	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000636	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945795	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0637	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004097	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	NarL
Synonyms	B1221^[2], NarR^[2], pleiotrophic regulation of anaerobic respiration: response regulator for nar, frd, dms and tor genes; (sensors, narX, narQ)^[2], FrdR^[2], nitrate/nitrite response regulator NarL^[2], unphosphorylated nitrate/nitrite response regulator NarL^[2], unmodified nitrate/nitrite response regulator NarL^[2] , ECK1215, frdR, JW1212, b1221
Product description	NarL transcriptional dual regulator^[2]^[3] Two-component regulator protein for nitrate/nitrite response; cognate sensor is NarX^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000156	two-component response regulator activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001789	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000156	two-component response regulator activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016032	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000160	two-component signal transduction system (phosphorelay)	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001789	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000160	two-component signal transduction system (phosphorelay)	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016032	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000160	two-component signal transduction system (phosphorelay)	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0902	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016032	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003700	transcription factor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000792	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005622	intracellular	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000792	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005622	intracellular	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016032	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006350	transcription	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0804	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000792	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001789	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016032	P	Seeded from EcoCyc (v14.0)	complete
	GO:0042128	nitrate assimilation	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0534	P	Seeded from EcoCyc (v14.0)	complete
	GO:0043565	sequence-specific DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000792	F	Seeded from EcoCyc (v14.0)	complete
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Interactions

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Partner Type	Partner	Notes	References	Evidence
Protein	yicI		PMID:16606699	Experiment(s):EBI-1139219
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Notes

Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSNQEPATIL LIDDHPMLRT GVKQLISMAP DITVVGEASN GEQGIELAES LDPDLILLDL NMPGMNGLET LDKLREKSLS GRIVVFSVSN HEEDVVTALK RGADGYLLKD MEPEDLLKAL HQAAAGEMVL SEALTPVLAA SLRANRATTE RDVNQLTPRE RDILKLIAQG LPNKMIARRL DITESTVKVH VKHMLKKMKL KSRVEAAVWV HQERIF
Length	216
Mol. Wt	23.927 kDa
pI	6.0 (calculated)
Extinction coefficient	6,990 (calc based on 1 Y, 1 W, and 0 C residues)
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Domains/Motifs/Modification Sites

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Type	Residues	Description	References
Domain	153..210	PF00196 Bacterial regulatory proteins, luxR family	PMID:19920124
Domain	9..121	PF00072 Response regulator receiver domain	PMID:19920124
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<motif_map/>

Structure
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Structures

<beststructure> gene=narL taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129184	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945795	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004097	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AF28	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10643	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10643	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945795	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000636	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0637	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.29E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	1667	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1224	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	799	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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Transcription unit(s)
narXL
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1275032..1275072 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1221 (EcoliWiki Page)	NCBI GEO profiles for narL
microarray	GenExpDB:b1221 (EcoliWiki Page)	Summary of data for narL from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to narL Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10643	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0637	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1221
EcoGene	EcoGene:EG10643	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000636	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004097	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT5G62920 (score: 1.000; bootstrap: 100%) AT3G48100 (score: 0.735) AT2G41310 (score: 0.379) AT3G57040 (score: 0.369) AT2G40670 (score: 0.346) AT3G56380 (score: 0.337) AT2G46790 (score: 0.065) AT2G07440 (score: 0.061)	From Inparanoid:20070104
Shigella flexneri	NARL	From SHIGELLACYC
E. coli O157	NARL	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00196 Bacterial regulatory proteins, luxR family
Superfamily (EcoliWiki Page)	SUPERFAMILY:46894
Superfamily (EcoliWiki Page)	SUPERFAMILY:52172
Panther (EcoliWiki Page)	PTHR26402:SF164
Pfam (EcoliWiki Page)	PF00072 Response regulator receiver domain
EcoCyc	EcoCyc:EG10643	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10643	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000636	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0637	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004097	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

narL:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to narL Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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