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Expression|

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Standard Name	narJ
Gene Synonym(s)	ECK1220, b1226, JW1217, chlC^[1]^[2]
Product Desc.	molybdenum cofactor assembly chaperone subunit (δ subunit) of nitrate reductase 1^[2]^[3] Redox enzyme maturation protein (REMP) for NarG; facilitates molybdenum insertion; binds NarG^[4]
Product Synonyms(s)	molybdenum-cofactor-assembly chaperone subunit (delta subunit) of nitrate reductase 1^[1], B1226^[2]^[1], ChlC^[2]^[1], NarJ^[2]^[1] , ECK1220, JW1217, b1226
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): narGHJI^[2], OP00060, chlC, narG, narGHIJ
Regulation/Activity
Quick Links
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Notes

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NarJ has two NarG binding sites: one for Mo insertion and one to block membrane attachment via NarI until activation is complete. Induced by anaerobiosis plus nitrate. NarJ is essential for a Tat-dependent interaction between NarG and Mo proteins.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	narJ
Mnemonic	Nitrate reductase, nitrate regulation
Synonyms	ECK1220, b1226, JW1217, chlC^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	27.68 minutes	MG1655: 1284362..1285072 <gbrowseImage> name=NC_000913:1284362..1285072 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1285289..1285999 <gbrowseImage> name=NC_012967:1285289..1285999 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1173083..1173793 <gbrowseImage> name=NC_012759:1173083..1173793 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1286716..1287426 <gbrowseImage> name=NC_007779:1286716..1287426 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1324603..1325313 <gbrowseImage> name=NC_010473:1324603..1325313 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele

Nt change(s)

AA change(s)

Phenotype: Type

Phenotype: Description

Reference

Availability

Comments

narJ(del) (Keio:JW1217)

deletion

PMID:16738554

Shigen

CGSC9103^[5]

nar(del)J737::kan

PMID:16738554

CGSC:102024

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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1217	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATCGAACTCGTGATTGTATC Primer 2:CCGTGCTGTCCTCCGGTGGTGAT
12H9	Kohara Phage	Genobase	PMID:3038334
4D8	Kohara Phage	Genobase	PMID:3038334
fadR13::Tn10	Linked marker	CAG18497 = CGSC7365^[5]	est. P1 cotransduction: 10% ^[6]
oppC506::Tn10	Linked marker	CAG12169 = CGSC7369^[5]	est. P1 cotransduction: 50% ^[6] Synonyms:zch-506::Tn10, zci-506::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10641	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10641	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000634	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945807	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0635	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004123	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	NarJ
Synonyms	molybdenum-cofactor-assembly chaperone subunit (delta subunit) of nitrate reductase 1^[1], B1226^[2]^[1], ChlC^[2]^[1], NarJ^[2]^[1] , ECK1220, JW1217, b1226
Product description	molybdenum cofactor assembly chaperone subunit (δ subunit) of nitrate reductase 1^[2]^[3] Redox enzyme maturation protein (REMP) for NarG; facilitates molybdenum insertion; binds NarG^[4]
EC number (for enzymes)	1.7.99.4^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0008940	nitrate reductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003765	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006457	protein folding				P	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0008940	nitrate reductase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:1.7.99.4	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009325	nitrate reductase complex	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003765	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042128	nitrate assimilation	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0534	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003765	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	sdhA	PMID:16606699	Experiment(s):EBI-1139229
Protein	yeaY	PMID:15690043	Experiment(s):EBI-880352
Protein	narG	PMID:15690043	Experiment(s):EBI-880352, EBI-880367, EBI-885776, EBI-885808
Protein	narH	PMID:15690043	Experiment(s):EBI-880352, EBI-885776
Protein	ompA	PMID:15690043	Experiment(s):EBI-880352
Protein	pepP	PMID:15690043	Experiment(s):EBI-880352
Protein	aceF	PMID:15690043	Experiment(s):EBI-885776
Protein	lpdA	PMID:15690043	Experiment(s):EBI-885776
Protein	narY	PMID:15690043	Experiment(s):EBI-885776
Protein	rplL	PMID:15690043	Experiment(s):EBI-885776
Protein	pepP	PMID:19402753	MALDI(Z-score):17.385217
Protein	narY	PMID:19402753	LCMS(ID Probability):99.6 MALDI(Z-score):22.455399
Protein	narH	PMID:19402753	LCMS(ID Probability):99.6 MALDI(Z-score):28.545841
Protein	narG	PMID:19402753	LCMS(ID Probability):99.6 MALDI(Z-score):38.955398
Protein	rplL	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MIELVIVSRL LEYPDAALWQ HQQEMFEAIA ASKNLPKEDA HALGIFLRDL TTMDPLDAQA QYSELFDRGR ATSLLLFEHV HGESRDRGQA MVDLLAQYEQ HGLQLNSREL PDHLPLYLEY LAQLPQSEAV EGLKDIAPIL ALLSARLQQR ESRYAVLFDL LLKLANTAID SDKVAEKIAD EARDDTPQAL DAVWEEEQVK FFADKGCGDS AITAHQRRFA GAVAPQYLNI TTGGQH
Length	236
Mol. Wt	26.449 kDa
pI	4.6 (calculated)
Extinction coefficient	21,430 - 21,555 (calc based on 7 Y, 2 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	3..165	PF02613 Nitrate reductase delta subunit		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=narJ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129189	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945807	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004123	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AF26	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10641	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10641	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945807	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000634	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0635	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	5.44+/-0.034	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	5^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	9	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
narGHJI
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1284342..1284382 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1226 (EcoliWiki Page)	NCBI GEO profiles for narJ
microarray	GenExpDB:b1226 (EcoliWiki Page)	Summary of data for narJ from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to narJ Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10641	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0635	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1226
EcoGene	EcoGene:EG10641	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000634	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004123	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	NARJ	From SHIGELLACYC
E. coli O157	NARJ	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02613 Nitrate reductase delta subunit
Superfamily (EcoliWiki Page)	SUPERFAMILY:89155
EcoCyc	EcoCyc:EG10641	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10641	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000634	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0635	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004123	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

narJ:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to narJ Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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