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Gene |

Product(s) |

Expression|

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Standard Name	narI
Gene Synonym(s)	ECK1221, b1227, JW1218, chlI^[1], chlI
Product Desc.	nitrate reductase A, γ subunit^[2]^[3]; Component of nitrate reductase A^[2]^[3] Nitrate reductase I (NRA), gamma subunit^[4]
Product Synonyms(s)	nitrate reductase 1, gamma (cytochrome b(NR)) subunit^[1], B1227^[2]^[1], ChlI^[2]^[1], NarI^[2]^[1] , chlI, ECK1221, JW1218, b1227
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): narGHJI^[2], OP00060, chlC, narG, narGHIJ
Regulation/Activity
Quick Links
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Notes

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NarI is involved in localizing NarGHI to the membrane Induced by anaerobiosis plus nitrate.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	narI
Mnemonic	Nitrate reductase, nitrate regulation
Synonyms	ECK1221, b1227, JW1218, chlI^[1], chlI
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	27.7 minutes	MG1655: 1285072..1285749 <gbrowseImage> name=NC_000913:1285072..1285749 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1285999..1286676 <gbrowseImage> name=NC_012967:1285999..1286676 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1173793..1174470 <gbrowseImage> name=NC_012759:1173793..1174470 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1287426..1288103 <gbrowseImage> name=NC_007779:1287426..1288103 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1325313..1325990 <gbrowseImage> name=NC_010473:1325313..1325990 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1285072	Edman degradation	PMID:3053688
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
narI(del) (Keio:JW1218)	deletion	deletion			PMID:16738554	Shigen CGSC9104^[5]
narI738(del)::kan					PMID:16738554	CGSC:102022
nar-204::Tn10			Enzyme Activity	Mutant is NarI^- and has reduced nitrate reductase activity	PMID:7047497	Experimental strain: RK5267	table 5
nar-204::Tn10			Enzyme Activity	Mutant is NarI^- and has reduced Formate activity	PMID:7047497	Experimental Strain: RK5267	table 5
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1218	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCAATTCCTGAATATGTTCTT Primer 2:CCGTGACGAGCGCGCACCAGCTG
12H9	Kohara Phage	Genobase	PMID:3038334
4D8	Kohara Phage	Genobase	PMID:3038334
fadR13::Tn10	Linked marker	CAG18497 = CGSC7365^[5]	est. P1 cotransduction: 9% ^[6]
oppC506::Tn10	Linked marker	CAG12169 = CGSC7369^[5]	est. P1 cotransduction: 51% ^[6] Synonyms:zch-506::Tn10, zci-506::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10640	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10640	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000633	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945808	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0634	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004125	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	NarI
Synonyms	nitrate reductase 1, gamma (cytochrome b(NR)) subunit^[1], B1227^[2]^[1], ChlI^[2]^[1], NarI^[2]^[1] , chlI, ECK1221, JW1218, b1227
Product description	nitrate reductase A, γ subunit^[2]^[3]; Component of nitrate reductase A^[2]^[3] Nitrate reductase I (NRA), gamma subunit^[4]
EC number (for enzymes)	1.7.99.4^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005506	iron ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0408	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005506	iron ion binding	PMID:9325288	IPI: Inferred from Physical Interaction		F	Seeded from EcoCyc (v14.0)	Missing: with/from
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008940	nitrate reductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003816	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008940	nitrate reductase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:1.7.99.4	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009055	electron carrier activity	PMID:10504245	IPI: Inferred from Physical Interaction		F	Seeded from EcoCyc (v14.0)	Missing: with/from
	GO:0009061	anaerobic respiration	PMID:7050087	IEP: Inferred from Expression Pattern		P	Seeded from EcoCyc (v14.0)	complete
	GO:0009325	nitrate reductase complex	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003816	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	F	Seeded from EcoCyc (v14.0)	complete
	GO:0017004	cytochrome complex assembly	PMID:3053688	IDA: Inferred from Direct Assay		P	Seeded from EcoCyc (v14.0)	complete
	GO:0022900	electron transport chain	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0249	P	Seeded from EcoCyc (v14.0)	complete
	GO:0031224	intrinsic to membrane	PMID:5996	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0042128	nitrate assimilation	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0534	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003816	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of nitrate reductase A	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 5 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
Inner Membrane		PMID:3053688	EchoLocation:narI
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MQFLNMFFFD IYPYIAGAVF LIGSWLRYDY GQYTWRAASS QMLDRKGMNL ASNLFHIGIL GIFVGHFFGM LTPHWMYEAW LPIEVKQKMA MFAGGASGVL CLIGGVLLLK RRLFSPRVRA TTTGADILIL SLLVIQCALG LLTIPFSAQH MDGSEMMKLV GWAQSVVTFH GGASQHLDGV AFIFRLHLVL GMTLFLLFPF SRLIHIWSVP VEYLTRKYQL VRARH
Length	225
Mol. Wt	25.498 kDa
pI	10.2 (calculated)
Extinction coefficient	44,920 - 45,170 (calc based on 8 Y, 6 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	3..225	PF02665 Nitrate reductase gamma subunit		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=narI taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129190	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945808	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004125	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P11350	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10640	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10640	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945808	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000633	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0634	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	20^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	22	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	6^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
narGHJI
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1285052..1285092 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1227 (EcoliWiki Page)	NCBI GEO profiles for narI
microarray	GenExpDB:b1227 (EcoliWiki Page)	Summary of data for narI from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to narI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10640	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0634	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1227
EcoGene	EcoGene:EG10640	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000633	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004125	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	NARI	From SHIGELLACYC
E. coli O157	NARI	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02665 Nitrate reductase gamma subunit
Superfamily (EcoliWiki Page)	SUPERFAMILY:103501
EcoCyc	EcoCyc:EG10640	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10640	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000633	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0634	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004125	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

narI:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to narI Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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