mutS:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

mutS

Gene Synonym(s)

ECK2728, b2733, JW2703, fdv, ant, plm[1], plm

Product Desc.

MutS[2][3];

Component of MutHLS complex, methyl-directed mismatch repair[2][3]

Methyl-directed mismatch repair protein; dimeric/tetrameric[4]

Product Synonyms(s)

methyl-directed mismatch repair protein[1], B2733[2][1], Plm[2][1], Ant[2][1], Fdv[2][1], MutS[2][1] , ant, ECK2728, fdv, JW2703, plm, b2733

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): mutS[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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MutS, also known as the "mismatch recognition" enzyme, is essential for the DNA mismatch repair (MMR) biological pathway. It recognizes base-base mismatches and small nucleotide insertion/deletion mispairs generated during DNA synthesis or damage caused by various agents. After recognizing the mismatches, MutS binds to the mismatch as a homodimer. Although the MutS binding site is sequence-wise identical, the mismatch and MutS are structurally and functionally different, so it is virtually a heterodimer.

MutS also functions as an ATPase, providing energy for the MMR pathway. After binding to the mismatch, a hemi-methylated dGATC site is located and cleaved by other MMR enzymes. This dGATC binding site is either in the 3' or 5' direction from the mismatch. The entire MMR process is a highly conserved pathway with great similarities between the human pathway and E. coli pathway. The human homolog, MutSα, acts virtually the same as the E. coli form of the protein.[5]

MutS has weak ATPase activity and may be involved in recognizing the DNA mismatch. MutS C-terminus dimerization, not tetramerization, is required for mismatch repair. [6] [4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mutS

Mnemonic

Mutator

Synonyms

ECK2728, b2733, JW2703, fdv, ant, plm[1], plm

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

61.54 minutes 

MG1655: 2855115..2857676
<gbrowseImage> name=NC_000913:2855115..2857676 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2751953..2754514
<gbrowseImage> name=NC_012967:2751953..2754514 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2740927..2743488
<gbrowseImage> name=NC_012759:2740927..2743488 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2855749..2858310
<gbrowseImage> name=NC_007779:2855749..2858310 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2947657..2950218
<gbrowseImage> name=NC_010473:2947657..2950218 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

mutS(del) (Keio:JW2703)

deletion

deletion

PMID:16738554

Shigen
CGSC10126[7]

mutS::Tn5KAN-I-SceI (FB20917)

Insertion at nt 2340 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20917

contains pKD46

mutS::Tn5KAN-I-SceI (FB20918)

Insertion at nt 2340 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20918

does not contain pKD46

mutS201::Tn5

CGSC:7794

mutS215::Tn10

CGSC:7797

mutS3

CGSC:11134

mutS210

CGSC:85420

mutS104::mini-Tn10

CGSC:85448

mutS738(del)::kan

PMID:16738554

CGSC:101976

MutS F596A

F596A

  • Mildly defective ATPase activity
  • Defective MutH activation in vitro
  • Moderately elevated mutation and DNA recombination rates in vivo

PMID:12606120

MutS H760A

H760A

  • Mildly defective ATPase activity
  • Defective MutH activation in vitro
  • Moderately elevated mutation and DNA recombination rates in vivo

PMID:12606120

MutS H728A

H728A

  • Mildly defective ATPase activity
  • Defective MutH activation in vitro
  • Moderately elevated mutation and DNA recombination rates in vivo

PMID:12606120

MutS S668A/T669A

S668A/T669A

  • Modest reduction of Kcat (~three-fold)
  • Fails to activate MMR

PMID:12606120

MutS D693N

D693N

  • Modest reduction of Kcat (~three-fold)
  • Fails to activate MMR

PMID:12606120

MutS S668A/T669A/E694A

S668A/T669A/E694A

  • Defective in DNA binding
  • Defective in MMR
  • Catalyzes ATP hydrolysis better than single E694A mutant

PMID:12606120

MutS K620M

K620M

  • Severely reduced ATP binding capacity
  • Fails to activate MutH in MMR initiation assays

PMID:12606120

MutS E694Q

E694Q

  • Binds ATP better than the wt MutS,but hydrolyzes ATP poorly
  • Supports low-level MutH activation in a mismatch-independent manner

PMID:12606120

MutS F36A

F36A

  • Fails to recognize a mismatch site hence failing to initiate MMR
  • Loses mismatch binding capacity

PMID:12606120

MutS D693A

D693A

  • Binds ATP better than the wt MutS,but hydrolyzes ATP poorly
  • Supports low-level MutH activation in a mismatch-independent manner

PMID:12606120

MutS E38Q

E38Q

  • Binds DNA better than wt MutS
  • Activates MutH ~50% more than the wt MutS in correlation with increased affinity for heteroduplex DNA
  • Completely inactive in MMR and in preventing homologous recombination in vivo

PMID:12606120

MutS E694A

E694A

  • Binds ATP better than the wt MutS,but hydrolyzes ATP poorly
  • Supports low-level MutH activation in a mismatch-independent manner

PMID:12606120

MutS E38A

E38A

  • Exhibits moderately increased affinity for homoduplex DNA in vitro
  • MMR defective in vivo

PMID:12606120

MutS E177A

E177A

  • Unable to form H bonds that link domains I and II
  • Indistinguishable from ΔmutS in the in vivo MMR and recombination assays
  • Possesses a lower affinity for heteroduplex DNA in vitro in the presence of ATP
  • Possesses a higher affinity for homoduplex DNA in vitro in the presence of ATP
  • Hyperactive in activating MutH with 2-fold and 4-fold increases observed relative to the wt MutS for heteroduplex and homoduplex DNA, respectively
  • Reduced ATP hydrolysis

PMID:12606120

MutS T115A

T115A

  • Unable to form H bonds that link domains I and II
  • Indistinguishable from ΔmutS in the in vivo MMR and recombination assays
  • Possesses a lower affinity for heteroduplex DNA in vitro in the presence of ATP
  • Possesses a higher affinity for homoduplex DNA in vitro in the presence of ATP
  • Hyperactive in activating MutH with 2-fold and 4-fold increases observed relative to the wt MutS for heteroduplex and homoduplex DNA, respectively
  • Reduced ATP hydrolysis

PMID:12606120

MutS R197E/R198E

R197E/R198E

  • Disrupts the domain interface more extensively than the E177A or T115A mutation
  • In vivo defects similar to E177A and T115A mutations
  • Relatively normal DNA binding
  • Slightly elevated affinity for homoduplex DNA in the presence of ATP
  • Completely fails to activate MutH in vitro

PMID:12606120

MutS

Overexpression of MutS

Mutation Frequence

The rate of GC transversion to TA is reduced.

PMID 10940054

See Fig 1

MutS R197E/R198E/R199E

R197E/R198E/R199E

  • Disrupts the domain interface more extensively than the E177A or T115A mutation
  • In vivo defects similar to E177A and T115A mutations
  • Probably results in an unstable protein, which is found in inclusion bodies when over-expressed

PMID:12606120

MutS

Overexpression of MutS

Mutation Frequence

the amount of mutations due to MutY are decreased.

PMID 10940054

See Fig 3

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2703

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGTGCAATAGAAAATTTCGA

Primer 2:CCaACCAGGCTCTTCAAGCGATA

12F2

Kohara Phage

Genobase

PMID:3038334

25D2

Kohara Phage

Genobase

PMID:3038334

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[7]

est. P1 cotransduction: 32% [8]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

cysI95::Tn10

Linked marker

CAG12173 = CGSC7425[7]

est. P1 cotransduction: 30% [8]
Synonyms:cysC95::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10625

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10625

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000618

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947206

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0620

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008977

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MutS

Synonyms

methyl-directed mismatch repair protein[1], B2733[2][1], Plm[2][1], Ant[2][1], Fdv[2][1], MutS[2][1] , ant, ECK2728, fdv, JW2703, plm, b2733

Product description

MutS[2][3];

Component of MutHLS complex, methyl-directed mismatch repair[2][3]

Methyl-directed mismatch repair protein; dimeric/tetrameric[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0006298

mismatch repair

PMID:6987663

IMP: Inferred from Mutant Phenotype

P

complete

GO:0030983

mismatched DNA binding

PMID:3014530

IDA: Inferred from Direct Assay

F

complete

GO:0006298

mismatch repair

PMID:2665076

IDA: Inferred from Direct Assay

P

complete

GO:0016887

ATPase activity

PMID:10715140

IDA: Inferred from Direct Assay

F

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0032300

mismatch repair complex

PMID:6987663

IGI: Inferred from Genetic Interaction

UniProtKB:P06722|UniProtKB:P23367

C

P06722 (MutH) P23367 (MutL)

complete

GO:0003684

damaged DNA binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00096

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00096

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000432

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005748

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007695

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007696

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007860

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007861

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016151

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0006298

mismatch repair

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00096

P

Seeded from EcoCyc (v14.0)

complete

GO:0006298

mismatch repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000432

P

Seeded from EcoCyc (v14.0)

complete

GO:0006298

mismatch repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005748

P

Seeded from EcoCyc (v14.0)

complete

GO:0006298

mismatch repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007695

P

Seeded from EcoCyc (v14.0)

complete

GO:0032136

adenine/cytosine mispair binding

PMID:11237867

IDA: Inferred from Direct Assay

F

complete

GO:0006298

mismatch repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007696

P

Seeded from EcoCyc (v14.0)

complete

GO:0006298

mismatch repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007860

P

Seeded from EcoCyc (v14.0)

complete

GO:0006298

mismatch repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007861

P

Seeded from EcoCyc (v14.0)

complete

GO:0006298

mismatch repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016151

P

Seeded from EcoCyc (v14.0)

complete

GO:0030983

mismatched DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000432

F

Seeded from EcoCyc (v14.0)

complete

GO:0030983

mismatched DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005748

F

Seeded from EcoCyc (v14.0)

complete

GO:0030983

mismatched DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007695

F

Seeded from EcoCyc (v14.0)

complete

GO:0030983

mismatched DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007696

F

Seeded from EcoCyc (v14.0)

complete

GO:0030983

mismatched DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007860

F

Seeded from EcoCyc (v14.0)

complete

GO:0030983

mismatched DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007861

F

Seeded from EcoCyc (v14.0)

complete

GO:0030983

mismatched DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016151

F

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

PMID:11967071

IEP: Inferred from Expression Pattern

P

complete

GO:0043531

ADP binding

PMID:11048711

IDA: Inferred from Direct Assay

F

Xstal structure

complete

GO:0030983

mismatched DNA binding

PMID:11048711

IDA: Inferred from Direct Assay

F

Xstal structure

complete

GO:0008301

DNA bending activity

PMID:14634210

IDA: Inferred from Direct Assay

F

atomic force microscopy

complete

GO:0006298

mismatch repair

PMID:14634210

IDA: Inferred from Direct Assay

P

Atomic force microscopy

complete

GO:0005524

ATP binding

PMID:12606120

IDA: Inferred from Direct Assay

F

complete

GO:0030983

mismatched DNA binding

PMID:3014530

IDA: Inferred from Direct Assay

F

Enzymatic and chemical protection (footprinting) assays

complete

GO:0000018

regulation of DNA recombination

PMID:2555716

IMP: Inferred from Mutant Phenotype

P

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of MutHLS complex, methyl-directed mismatch repair

PMID:6987663

Inferred from genetic interaction

Protein

tufA

PMID:15690043

Experiment(s):EBI-886447

Protein

galF

PMID:16606699

Experiment(s):EBI-1143773

Protein

baeS

PMID:16606699

Experiment(s):EBI-1143773

Protein

envZ

PMID:16606699

Experiment(s):EBI-1143773

Protein

tufB

PMID:19402753

MALDI(Z-score):19.762370

Protein

DnaN: beta sliding clamp subunit of DNA pol III

PMID:11459978

Protein mobility shift during native gel electrophoresis and kinase protection assay.

DNA

GT mismatches

PMID:11048711

Crystal structure

Protein

MutL

PMID:9250691

Visualized using EM

DNA

Heteroduplex DNA

PMID:9250691

Visualized using EM

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSAIENFDAH TPMMQQYLRL KAQHPEILLF YRMGDFYELF YDDAKRASQL LDISLTKRGA
SAGEPIPMAG IPYHAVENYL AKLVNQGESV AICEQIGDPA TSKGPVERKV VRIVTPGTIS
DEALLQERQD NLLAAIWQDS KGFGYATLDI SSGRFRLSEP ADRETMAAEL QRTNPAELLY
AEDFAEMSLI EGRRGLRRRP LWEFEIDTAR QQLNLQFGTR DLVGFGVENA PRGLCAAGCL
LQYAKDTQRT TLPHIRSITM EREQDSIIMD AATRRNLEIT QNLAGGAENT LASVLDCTVT
PMGSRMLKRW LHMPVRDTRV LLERQQTIGA LQDFTAGLQP VLRQVGDLER ILARLALRTA
RPRDLARMRH AFQQLPELRA QLETVDSAPV QALREKMGEF AELRDLLERA IIDTPPVLVR
DGGVIASGYN EELDEWRALA DGATDYLERL EVRERERTGL DTLKVGFNAV HGYYIQISRG
QSHLAPINYM RRQTLKNAER YIIPELKEYE DKVLTSKGKA LALEKQLYEE LFDLLLPHLE
ALQQSASALA ELDVLVNLAE RAYTLNYTCP TFIDKPGIRI TEGRHPVVEQ VLNEPFIANP
LNLSPQRRML IITGPNMGGK STYMRQTALI ALMAYIGSYV PAQKVEIGPI DRIFTRVGAA
DDLASGRSTF MVEMTETANI LHNATEYSLV LMDEIGRGTS TYDGLSLAWA CAENLANKIK
ALTLFATHYF ELTQLPEKME GVANVHLDAL EHGDTIAFMH SVQDGAASKS YGLAVAALAG
VPKEVIKRAR QKLRELESIS PNAAATQVDG TQMSLLSVPE ETSPAVEALE NLDPDSLTPR
QALEWIYRLK SLV
Length

853

Mol. Wt

95.249 kDa

pI

5.4 (calculated)

Extinction coefficient

73,230 - 73,980 (calc based on 27 Y, 6 W, and 6 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

11..123

PF01624 MutS domain I

PMID:19920124

Domain

131..256

PF05188 MutS domain II

PMID:19920124

Domain

264..564

PF05192 MutS domain III

PMID:19920124

Domain

428..519

PF05190 MutS family domain IV

PMID:19920124

Domain

567..799

PF00488 MutS domain V

PMID:19920124

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mutS taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Analytical ultracentrifugation data suggest that MutS exists as an equilibrating mixture of dimers and tetramers, both of which would be present at physiologically relevant concentrations. Kinetic parameters for ATPase activity and oligomerization are described[9][10]

There are several structure papers for MutS:

  • The crystal structure of DNA mismatch repair protein MutS binding to a G-T mismatch[11]
  • Crystal structures of mismatch repair protein MutS and its complex with a substrate DNA[12]

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

Purification protocol

from plasmid pMS312

PMID:3014530

Purification protocol

Single-step purification of His6-MutS

PMID:8747662

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130640

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947206

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008977

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P23909

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10625

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10625

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947206

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000618

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0620

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

8.80E+01

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

1049

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

150

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

484

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

mutS

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2855095..2855135 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2733 (EcoliWiki Page)

NCBI GEO profiles for mutS

microarray

GenExpDB:b2733 (EcoliWiki Page)

Summary of data for mutS from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (2854755..2855144) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ12; Well:E1[13]

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Notes

Accessions Related to mutS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10625

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0620

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2733

EcoGene

EcoGene:EG10625

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000618

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008977

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000014707 (score: 1.000; bootstrap: 62%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000013289 (score: 1.000; bootstrap: 85%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT3G24495 (score: 1.000; bootstrap: 62%)
  • AT4G02070 (score: 0.103)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000001867 (score: 1.000; bootstrap: 89%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00035331 (score: 1.000; bootstrap: 67%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00003422 (score: 1.000; bootstrap: 51%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000003882 (score: 1.000; bootstrap: 56%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000008912 (score: 1.000; bootstrap: 73%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-020905-3 (score: 1.000; bootstrap: 68%)
  • ZDB-CDNA-040425-552 (score: 1.000; bootstrap: 69%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0232360 (score: 1.000; bootstrap: 98%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0036486 (score: 1.000; bootstrap: 61%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA20021-PA (score: 1.000; bootstrap: 64%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000014557 (score: 1.000; bootstrap: 56%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000234420 (score: 1.000; bootstrap: 55%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000024039 (score: 1.000; bootstrap: 56%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000001344 (score: 1.000; bootstrap: 62%)

From Inparanoid:20070104

Mus musculus

  • MGI:1343961 (score: 1.000; bootstrap: 50%)

From Inparanoid:20070104

Oryza gramene

  • Q5VR41 (score: 1.000; bootstrap: 80%)
  • Q8RVT1 (score: 0.676)
  • Q9XGD0 (score: 0.608)
  • Q9T0N6 (score: 0.510)
  • Q69MX6 (score: 0.075)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000020434 (score: 1.000; bootstrap: 78%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000018449 (score: 1.000; bootstrap: 56%)
  • ENSRNOP00000021923 (score: 1.000; bootstrap: 54%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YHR120W (score: 1.000; bootstrap: 78%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPCC2856c (score: 1.000; bootstrap: 50%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000166199 (score: 1.000; bootstrap: 67%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00028603001 (score: 1.000; bootstrap: 84%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000042811 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

MUTS

From SHIGELLACYC

E. coli O157

MUTS

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF05190 MutS family domain IV

Pfam (EcoliWiki Page)

PF01624 MutS domain I

Pfam (EcoliWiki Page)

PF05188 MutS domain II

Pfam (EcoliWiki Page)

PF05192 MutS domain III

Pfam (EcoliWiki Page)

PF00488 MutS domain V

Superfamily (EcoliWiki Page)

SUPERFAMILY:48334

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

Superfamily (EcoliWiki Page)

SUPERFAMILY:53150

Panther (EcoliWiki Page)

PTHR11361:SF34

Superfamily (EcoliWiki Page)

SUPERFAMILY:55271

EcoCyc

EcoCyc:EG10625

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10625

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000618

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0620

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008977

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Li, GM (2008) Mechanisms and functions of DNA mismatch repair. Cell Res. 18 85-98 PubMed
  6. ↑ Mendillo, ML et al. (2007) Escherichia coli MutS tetramerization domain structure reveals that stable dimers but not tetramers are essential for DNA mismatch repair in vivo. J. Biol. Chem. 282 16345-54 PubMed
  7. ↑ 7.0 7.1 7.2 CGSC: The Coli Genetics Stock Center
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  9. ↑ Bjornson, KP et al. (2003) Assembly and molecular activities of the MutS tetramer. J. Biol. Chem. 278 34667-73 PubMed
  10. ↑ Bjornson, KP et al. (2000) Modulation of MutS ATP hydrolysis by DNA cofactors. Biochemistry 39 3176-83 PubMed
  11. ↑ Lamers, MH et al. (2000) The crystal structure of DNA mismatch repair protein MutS binding to a G x T mismatch. Nature 407 711-7 PubMed
  12. ↑ Obmolova, G et al. (2000) Crystal structures of mismatch repair protein MutS and its complex with a substrate DNA. Nature 407 703-10 PubMed
  13. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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