mutS:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
MutS |
|---|---|
| Synonyms |
methyl-directed mismatch repair protein[1], B2733[2][1], Plm[2][1], Ant[2][1], Fdv[2][1], MutS[2][1] , ant, ECK2728, fdv, JW2703, plm, b2733 |
| Product description |
Component of MutHLS complex, methyl-directed mismatch repair[2][3] Methyl-directed mismatch repair protein; dimeric/tetrameric[4] |
| EC number (for enzymes) |
|
| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0006298 |
mismatch repair |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0030983 |
mismatched DNA binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0006298 |
mismatch repair |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0016887 |
ATPase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0032300 |
mismatch repair complex |
IGI: Inferred from Genetic Interaction |
C |
P06722 (MutH) P23367 (MutL) |
complete | |||
| GO:0003684 |
damaged DNA binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006298 |
mismatch repair |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006298 |
mismatch repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006298 |
mismatch repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006298 |
mismatch repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0032136 |
adenine/cytosine mispair binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0006298 |
mismatch repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006298 |
mismatch repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006298 |
mismatch repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006298 |
mismatch repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0030983 |
mismatched DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0030983 |
mismatched DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0030983 |
mismatched DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0030983 |
mismatched DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0030983 |
mismatched DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0030983 |
mismatched DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0030983 |
mismatched DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006974 |
response to DNA damage stimulus |
IEP: Inferred from Expression Pattern |
P |
complete | ||||
| GO:0043531 |
ADP binding |
IDA: Inferred from Direct Assay |
F |
Xstal structure |
complete | |||
| GO:0030983 |
mismatched DNA binding |
IDA: Inferred from Direct Assay |
F |
Xstal structure |
complete | |||
| GO:0008301 |
DNA bending activity |
IDA: Inferred from Direct Assay |
F |
atomic force microscopy |
complete | |||
| GO:0006298 |
mismatch repair |
IDA: Inferred from Direct Assay |
P |
Atomic force microscopy |
complete | |||
| GO:0005524 |
ATP binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0030983 |
mismatched DNA binding |
IDA: Inferred from Direct Assay |
F |
Enzymatic and chemical protection (footprinting) assays |
complete | |||
| GO:0000018 |
regulation of DNA recombination |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of MutHLS complex, methyl-directed mismatch repair |
Inferred from genetic interaction | ||
|
Protein |
tufA |
Experiment(s):EBI-886447 | ||
|
Protein |
galF |
Experiment(s):EBI-1143773 | ||
|
Protein |
baeS |
Experiment(s):EBI-1143773 | ||
|
Protein |
envZ |
Experiment(s):EBI-1143773 | ||
|
Protein |
tufB |
MALDI(Z-score):19.762370 | ||
|
Protein |
DnaN: beta sliding clamp subunit of DNA pol III |
Protein mobility shift during native gel electrophoresis and kinase protection assay. | ||
|
DNA |
GT mismatches |
Crystal structure | ||
|
Protein |
MutL |
Visualized using EM | ||
|
DNA |
Heteroduplex DNA |
Visualized using EM | ||
| edit table |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MSAIENFDAH TPMMQQYLRL KAQHPEILLF YRMGDFYELF YDDAKRASQL LDISLTKRGA SAGEPIPMAG IPYHAVENYL AKLVNQGESV AICEQIGDPA TSKGPVERKV VRIVTPGTIS DEALLQERQD NLLAAIWQDS KGFGYATLDI SSGRFRLSEP ADRETMAAEL QRTNPAELLY AEDFAEMSLI EGRRGLRRRP LWEFEIDTAR QQLNLQFGTR DLVGFGVENA PRGLCAAGCL LQYAKDTQRT TLPHIRSITM EREQDSIIMD AATRRNLEIT QNLAGGAENT LASVLDCTVT PMGSRMLKRW LHMPVRDTRV LLERQQTIGA LQDFTAGLQP VLRQVGDLER ILARLALRTA RPRDLARMRH AFQQLPELRA QLETVDSAPV QALREKMGEF AELRDLLERA IIDTPPVLVR DGGVIASGYN EELDEWRALA DGATDYLERL EVRERERTGL DTLKVGFNAV HGYYIQISRG QSHLAPINYM RRQTLKNAER YIIPELKEYE DKVLTSKGKA LALEKQLYEE LFDLLLPHLE ALQQSASALA ELDVLVNLAE RAYTLNYTCP TFIDKPGIRI TEGRHPVVEQ VLNEPFIANP LNLSPQRRML IITGPNMGGK STYMRQTALI ALMAYIGSYV PAQKVEIGPI DRIFTRVGAA DDLASGRSTF MVEMTETANI LHNATEYSLV LMDEIGRGTS TYDGLSLAWA CAENLANKIK ALTLFATHYF ELTQLPEKME GVANVHLDAL EHGDTIAFMH SVQDGAASKS YGLAVAALAG VPKEVIKRAR QKLRELESIS PNAAATQVDG TQMSLLSVPE ETSPAVEALE NLDPDSLTPR QALEWIYRLK SLV |
| Length |
853 |
| Mol. Wt |
95.249 kDa |
| pI |
5.4 (calculated) |
| Extinction coefficient |
73,230 - 73,980 (calc based on 27 Y, 6 W, and 6 C residues) |
| edit table |
Domains/Motifs/Modification Sites
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See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
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Structure
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Structure figures<protect> | ||||||
Notes
Analytical ultracentrifugation data suggest that MutS exists as an equilibrating mixture of dimers and tetramers, both of which would be present at physiologically relevant concentrations. Kinetic parameters for ATPase activity and oligomerization are described[21][8]
There are several structure papers for MutS:
- The crystal structure of DNA mismatch repair protein MutS binding to a G-T mismatch[11]
- Crystal structures of mismatch repair protein MutS and its complex with a substrate DNA[22]
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
|
Purification protocol |
from plasmid pMS312 |
|
|
Purification protocol |
Single-step purification of His6-MutS |
|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 Glickman, BW & Radman, M (1980) Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction. Proc. Natl. Acad. Sci. U.S.A. 77 1063-7 PubMed
- ↑ 6.0 6.1 6.2 Su, SS & Modrich, P (1986) Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs. Proc. Natl. Acad. Sci. U.S.A. 83 5057-61 PubMed
- ↑ Lahue, RS et al. (1989) DNA mismatch correction in a defined system. Science 245 160-4 PubMed
- ↑ 8.0 8.1 Bjornson, KP et al. (2000) Modulation of MutS ATP hydrolysis by DNA cofactors. Biochemistry 39 3176-83 PubMed
- ↑ Brown, J et al. (2001) Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches. Biochem. J. 354 627-33 PubMed
- ↑ Khil, PP & Camerini-Otero, RD (2002) Over 1000 genes are involved in the DNA damage response of Escherichia coli. Mol. Microbiol. 44 89-105 PubMed
- ↑ 11.0 11.1 11.2 11.3 Lamers, MH et al. (2000) The crystal structure of DNA mismatch repair protein MutS binding to a G x T mismatch. Nature 407 711-7 PubMed
- ↑ 12.0 12.1 Wang, H et al. (2003) DNA bending and unbending by MutS govern mismatch recognition and specificity. Proc. Natl. Acad. Sci. U.S.A. 100 14822-7 PubMed
- ↑ Junop, MS et al. (2003) In vitro and in vivo studies of MutS, MutL and MutH mutants: correlation of mismatch repair and DNA recombination. DNA Repair (Amst.) 2 387-405 PubMed
- ↑ Rayssiguier, C et al. (1989) The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants. Nature 342 396-401 PubMed
- ↑ Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 16.0 16.1 16.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ López de Saro, FJ & O'Donnell, M (2001) Interaction of the beta sliding clamp with MutS, ligase, and DNA polymerase I. Proc. Natl. Acad. Sci. U.S.A. 98 8376-80 PubMed
- ↑ 19.0 19.1 Allen, DJ et al. (1997) MutS mediates heteroduplex loop formation by a translocation mechanism. EMBO J. 16 4467-76 PubMed
- ↑ 20.0 20.1 20.2 20.3 20.4 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
- ↑ Bjornson, KP et al. (2003) Assembly and molecular activities of the MutS tetramer. J. Biol. Chem. 278 34667-73 PubMed
- ↑ Obmolova, G et al. (2000) Crystal structures of mismatch repair protein MutS and its complex with a substrate DNA. Nature 407 703-10 PubMed
- ↑ Feng, G & Winkler, ME (1995) Single-step purifications of His6-MutH, His6-MutL and His6-MutS repair proteins of escherichia coli K-12. BioTechniques 19 956-65 PubMed
Categories
- GO:0006298 ! mismatch repair
- GO:0030983 ! mismatched DNA binding
- GO:0016887 ! ATPase activity
- GO:0000166 ! nucleotide binding
- GO:0032300 ! mismatch repair complex
- GO:0003684 ! damaged DNA binding
- GO:0005524 ! ATP binding
- GO:0032136 ! adenine/cytosine mispair binding
- GO:0006974 ! cellular response to DNA damage stimulus
- GO:0043531 ! ADP binding
- GO:0008301 ! DNA binding, bending
- GO:0000018 ! regulation of DNA recombination
- Proteins
- RefGenome Annotated Gene
