murI:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
murI |
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Gene Synonym(s) |
ECK3959, b3967, JW5550, dga, glr, yijA[1], yijA |
Product Desc. |
Component of glutamate racemase[2][3] Glutamate racemase; D-glutamate synthesis;[4] |
Product Synonyms(s) |
glutamate racemase[1], B3967[2][1], YijA[2][1], Glr[2][1], Dga[2][1], MurI[2][1] , dga, ECK3959, glr, JW5550, yijA, b3967 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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Notes
murI is an essential gene. MurI can inhibit DNA gyrase activity (Ashiuchi, 2002).[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
murI |
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Mnemonic |
Murein |
Synonyms |
ECK3959, b3967, JW5550, dga, glr, yijA[1], yijA |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
89.74 minutes |
MG1655: 4163451..4164308 |
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NC_012967: 4145289..4146146 |
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NC_012759: 4053120..4053977 |
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W3110 |
|
W3110: 3471253..3470396 |
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DH10B: 4263148..4264005 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW5550 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCGCTACCAAACTGCAGGACGG Primer 2:CCGCCTAAAACTGCCAGTTTTTC | |
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 69% [6] | ||
Linked marker |
est. P1 cotransduction: 30% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG11204 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11204 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001184 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB1189 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0012989 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
MurI |
---|---|
Synonyms |
glutamate racemase[1], B3967[2][1], YijA[2][1], Glr[2][1], Dga[2][1], MurI[2][1] , dga, ECK3959, glr, JW5550, yijA, b3967 |
Product description |
Component of glutamate racemase[2][3] Glutamate racemase; D-glutamate synthesis;[4] |
EC number (for enzymes) | |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0005737 |
cytoplasm |
C |
Seeded from Riley et al 2006 [1]. |
Missing: evidence, reference | ||||
GO:0007047 |
cellular cell wall organization |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0961 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008152 |
metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001920 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008360 |
regulation of cell shape |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0133 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008881 |
glutamate racemase activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00258 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008881 |
glutamate racemase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004391 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008881 |
glutamate racemase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
EC:5.1.1.3 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009252 |
peptidoglycan biosynthetic process |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00258 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009252 |
peptidoglycan biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004391 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009252 |
peptidoglycan biosynthetic process |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0573 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016853 |
isomerase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0413 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016855 |
racemase and epimerase activity, acting on amino acids and derivatives |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001920 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016855 |
racemase and epimerase activity, acting on amino acids and derivatives |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR018187 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of glutamate racemase |
could be indirect |
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Protein |
tufA |
PMID:15690043 |
Experiment(s):EBI-888865 | |
Protein |
nusG |
PMID:15690043 |
Experiment(s):EBI-893999 | |
Protein |
rplK |
PMID:15690043 |
Experiment(s):EBI-893999 | |
Protein |
rplL |
PMID:15690043 |
Experiment(s):EBI-893999 | |
Protein |
rpsB |
PMID:15690043 |
Experiment(s):EBI-893999 | |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MRQSMATKLQ DGNTPCLAAT PSEPRPTVLV FDSGVGGLSV YDEIRHLLPD LHYIYAFDNV AFPYGEKSEA FIVERVVAIV TAVQERYPLA LAVVACNTAS TVSLPALREK FDFPVVGVVP AIKPAARLTA NGIVGLLATR GTVKRSYTHE LIARFANECQ IEMLGSAEMV ELAEAKLHGE DVSLDALKRI LRPWLRMKEP PDTVVLGCTH FPLLQEELLQ VLPEGTRLVD SGAAIARRTA WLLEHEAPDA KSADANIAFC MAMTPGAEQL LPVLQRYGFE TLEKLAVLG |
Length |
289 |
Mol. Wt |
31.504 kDa |
pI |
5.3 (calculated) |
Extinction coefficient |
21,430 - 22,055 (calc based on 7 Y, 2 W, and 5 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0012989 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG11204 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11204 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120001184 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1189 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
Ecoli K-12 |
13.395+/-0.076 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.017502917 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
Protein |
E. coli K-12 MG1655 |
989 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
159 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
424 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
![]() Figure courtesy of RegulonDB |
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
murI inactivation leads to an inability to form colonies in the absence of D-glutamic acid. [7]
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:4163431..4163471
source=MG1655
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for murI | |
microarray |
Summary of data for murI from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to murI Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG11204 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1189 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11204 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001184 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0012989 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Xenopus tropicalis |
|
From Inparanoid:20070104 |
Shigella flexneri |
MURI |
From SHIGELLACYC |
E. coli O157 |
MURI |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG11204 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11204 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001184 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1189 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0012989 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Doublet, P et al. (1992) Identification of the Escherichia coli murI gene, which is required for the biosynthesis of D-glutamic acid, a specific component of bacterial peptidoglycan. J. Bacteriol. 174 5772-9 PubMed
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