murA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

murA

Gene Synonym(s)

ECK3178, b3189, JW3156, mrbA, murZ[1], murZ

Product Desc.

UDP-N-acetylglucosamine enoylpyruvyl transferase; fosfomycin resistance[2]

Product Synonyms(s)

UDP-N-acetylglucosamine 1-carboxyvinyltransferase[1], B3189[3][1], MurZ[3][1], MrbA[3][1], MurA[3][1] , ECK3178, JW3156, mrbA, murZ, b3189

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): murA[3]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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murA is an essential gene.[2]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

murA

Mnemonic

Murein

Synonyms

ECK3178, b3189, JW3156, mrbA, murZ[1], murZ

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

71.84 minutes 

MG1655: 3334516..3333257
<gbrowseImage> name=NC_000913:3333257..3334516 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3271729..3270470
<gbrowseImage> name=NC_012967:3270470..3271729 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3220405..3221664
<gbrowseImage> name=NC_012759:3220405..3221664 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3336349..3335090
<gbrowseImage> name=NC_007779:3335090..3336349 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3333257

Edman degradation

PMID:1512209


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Strain DH10B

I28N

Nonsynonomous mutation

PMID:18245285

ASAP

This is a SNP in E. coli K-12 Strain DH10B

murA C115D

C115D

Resistant to

Fosfomycin

PMID:8075065

murZ Fosr

Mapped to 69.3 min

Resistant to

Resistant to phosphomycin

PMID:1512209

See figure 1 for chemical reaction.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3156

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGATAAATTTCGTGTTCAGGG

Primer 2:CCTTCGCCTTTCACACGCTCAAT

14F11

Kohara Phage

Genobase

PMID:3038334

sfsB203::Tn10

Linked marker

CAG12072 = CGSC7440[4]

est. P1 cotransduction: 94% [5]
Synonyms:zgj-203::Tn10, zha-203::Tn10

zhc-6::Tn10

Linked marker

CAG12153 = CGSC7442[4]

est. P1 cotransduction: 30% [5]
Synonyms:zha-6::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11358

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11358

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001327

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947703

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1333

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010479

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MurA

Synonyms

UDP-N-acetylglucosamine 1-carboxyvinyltransferase[1], B3189[3][1], MurZ[3][1], MrbA[3][1], MurA[3][1] , ECK3178, JW3156, mrbA, murZ, b3189

Product description

UDP-N-acetylglucosamine enoylpyruvyl transferase; fosfomycin resistance[2]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00111

C

Seeded from EcoCyc (v14.0)

complete

GO:0008760

UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity

PMID:1512209

IDA: Inferred from Direct Assay

F

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0008760

UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity

PMID:8075064

IDA: Inferred from Direct Assay

F

PMID:7608103 states MurZ is MurA

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0007047

cellular cell wall organization

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0961

P

Seeded from EcoCyc (v14.0)

complete

GO:0007049

cell cycle

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0131

P

Seeded from EcoCyc (v14.0)

complete

GO:0008360

regulation of cell shape

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0133

P

Seeded from EcoCyc (v14.0)

complete

GO:0008760

UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00111

F

Seeded from EcoCyc (v14.0)

complete

GO:0008760

UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.5.1.7

F

Seeded from EcoCyc (v14.0)

complete

GO:0009252

peptidoglycan biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00111

P

Seeded from EcoCyc (v14.0)

complete

GO:0009252

peptidoglycan biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0573

P

Seeded from EcoCyc (v14.0)

complete

GO:0019277

UDP-N-acetylgalactosamine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005750

P

Seeded from EcoCyc (v14.0)

complete

GO:0051301

cell division

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0132

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

prsA

PMID:15690043

Experiment(s):EBI-888887

Protein

ftsI

PMID:15690043

Experiment(s):EBI-888887

Protein

tufA

PMID:15690043

Experiment(s):EBI-888887

Protein

rplV

PMID:15690043

Experiment(s):EBI-894012

Protein

rpsB

PMID:15690043

Experiment(s):EBI-894012

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-894012

Protein

yhgF

PMID:15690043

Experiment(s):EBI-894012

Protein

wzc

PMID:15690043

Experiment(s):EBI-894012

Protein

degQ

PMID:15690043

Experiment(s):EBI-894012

Protein

rapA

PMID:15690043

Experiment(s):EBI-894012

Protein

pepB

PMID:15690043

Experiment(s):EBI-894012

Protein

rplD

PMID:15690043

Experiment(s):EBI-894012

Protein

rplK

PMID:15690043

Experiment(s):EBI-894012

Protein

rplL

PMID:15690043

Experiment(s):EBI-894012

Protein

rplM

PMID:15690043

Experiment(s):EBI-894012

Protein

umuC

PMID:16606699

Experiment(s):EBI-1145112

Protein

glnE

PMID:16606699

Experiment(s):EBI-1145112

Protein

nadE

PMID:16606699

Experiment(s):EBI-1145112

Protein

matC

PMID:16606699

Experiment(s):EBI-1145112

Protein

uvrA

PMID:16606699

Experiment(s):EBI-1145112

Protein

rapA

PMID:19402753

LCMS(ID Probability):99.0

Protein

dnaN

PMID:19402753

MALDI(Z-score):36.388166

Protein

yhgF

PMID:19402753

LCMS(ID Probability):99.0

Protein

prs

PMID:19402753

MALDI(Z-score):33.865689

Protein

degQ

PMID:19402753

LCMS(ID Probability):99.0

Protein

wzc

PMID:19402753

LCMS(ID Probability):99.0

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MDKFRVQGPT KLQGEVTISG AKNAALPILF AALLAEEPVE IQNVPKLKDV DTSMKLLSQL
GAKVERNGSV HIDARDVNVF CAPYDLVKTM RASIWALGPL VARFGQGQVS LPGGCTIGAR
PVDLHISGLE QLGATIKLEE GYVKASVDGR LKGAHIVMDK VSVGATVTIM CAATLAEGTT
IIENAAREPE IVDTANFLIT LGAKISGQGT DRIVIEGVER LGGGVYRVLP DRIETGTFLV
AAAISRGKII CRNAQPDTLD AVLAKLRDAG ADIEVGEDWI SLDMHGKRPK AVNVRTAPHP
AFPTDMQAQF TLLNLVAEGT GFITETVFEN RFMHVPELSR MGAHAEIESN TVICHGVEKL
SGAQVMATDL RASASLVLAG CIAEGTTVVD RIYHIDRGYE RIEDKLRALG ANIERVKGE
Length

419

Mol. Wt

44.817 kDa

pI

6.1 (calculated)

Extinction coefficient

18,450 - 19,200 (calc based on 5 Y, 2 W, and 6 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

6..406

PF00275 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=murA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131079

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947703

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010479

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A749

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11358

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11358

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947703

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001327

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1333

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

4.10E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

50.691+/-0.392

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.12192216

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

4855

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

887

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2445

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

murA

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3334496..3334536 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3189 (EcoliWiki Page)

NCBI GEO profiles for murA

microarray

GenExpDB:b3189 (EcoliWiki Page)

Summary of data for murA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (3334042..3334193) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ18; Well:H9[6]

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Notes

Accessions Related to murA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11358

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1333

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3189

EcoGene

EcoGene:EG11358

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001327

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010479

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Xenopus tropicalis

  • ENSXETP00000042261 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

MURA

From SHIGELLACYC

E. coli O157

MURA

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00275 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Panther (EcoliWiki Page)

PTHR21090:SF4

Superfamily (EcoliWiki Page)

SUPERFAMILY:55205

EcoCyc

EcoCyc:EG11358

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11358

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001327

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1333

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010479

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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