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Gene |

Product(s) |

Expression|

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Standard Name	msrA
Gene Synonym(s)	ECK4215, b4219, JW4178, pmsR, pms^[1], pms
Product Desc.	methionine sulfoxide reductase A^[2]^[3] Methionine sulfoxide reductase A; specific for S diastereoisomers^[4]
Product Synonyms(s)	methionine sulfoxide reductase A^[1], B4219^[2]^[1], Pms^[2]^[1], PmsR^[2]^[1], MsrA^[2]^[1] , ECK4215, JW4178, pms, pmsR, b4219
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): msrA^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	msrA
Mnemonic	Methionine sulfoxide reductase
Synonyms	ECK4215, b4219, JW4178, pmsR, pms^[1], pms
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	95.69 minutes	MG1655: 4440199..4439561 <gbrowseImage> name=NC_000913:4439561..4440199 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4425933..4425295 <gbrowseImage> name=NC_012967:4425295..4425933 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4378296..4378934 <gbrowseImage> name=NC_012759:4378296..4378934 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4446856..4446218 <gbrowseImage> name=NC_007779:4446218..4446856 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4540561..4539923 <gbrowseImage> name=NC_010473:4539923..4540561 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	4439564	Edman degradation	PMID:1386361
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔmsrA (Keio:JW4178)	deletion	deletion		PMID:16738554	Shigen CGSC11008^[5]
msrAC52S		C52S	Loss of activity			seeded from UniProt:P0A744
msrAC87S		C87S	No effect			seeded from UniProt:P0A744
msrAC199S		C199S	Decrease in activity			seeded from UniProt:P0A744
msrAC207S		C207S	Decrease in activity			seeded from UniProt:P0A744
ΔmsrA768::kan				PMID:16738554	CGSC:101958
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4178	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGTTTATTTGATAAAAAGCA Primer 2:CCTGCTTCCGGCGGCAGACAGAC
5B5	Kohara Phage	Genobase	PMID:3038334
E1H3	Kohara Phage	Genobase	PMID:3038334
cycA30::Tn10	Linked marker	CAG12073 = CGSC7482^[5]	est. P1 cotransduction: 63% ^[6]
zjf-920::Tn10	Linked marker	CAG12019 = CGSC7484^[5]	est. P1 cotransduction: 84% ^[6] Synonyms:zjh-901::Tn10, zjg-920::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11433	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11433	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001397	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948734	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1403	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013804	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MsrA
Synonyms	methionine sulfoxide reductase A^[1], B4219^[2]^[1], Pms^[2]^[1], PmsR^[2]^[1], MsrA^[2]^[1] , ECK4215, JW4178, pms, pmsR, b4219
Product description	methionine sulfoxide reductase A^[2]^[3] Methionine sulfoxide reductase A; specific for S diastereoisomers^[4]
EC number (for enzymes)	1.8.4.6^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0006464	protein modification process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01401	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008113	peptide-methionine-(S)-S-oxide reductase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01401	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008113	peptide-methionine-(S)-S-oxide reductase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:1.8.4.11	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016671	oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002569	F	Seeded from EcoCyc (v14.0)	complete
	GO:0019538	protein metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002569	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002569	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	lrp		PMID:16606699	Experiment(s):EBI-1147692
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSLFDKKHLV SPADALPGRN TPMPVATLHA VNGHSMTNVP DGMEIAIFAM GCFWGVERLF WQLPGVYSTA AGYTGGYTPN PTYREVCSGD TGHAEAVRIV YDPSVISYEQ LLQVFWENHD PAQGMRQGND HGTQYRSAIY PLTPEQDAAA RASLERFQAA MLAADDDRHI TTEIANATPF YYAEDDHQQY LHKNPYGYCG IGGIGVCLPP EA
Length	212
Mol. Wt	23.314 kDa
pI	4.9 (calculated)
Extinction coefficient	35,870 - 36,370 (calc based on 13 Y, 3 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0A744
Domain	44..202	PF01625 Peptide methionine sulfoxide reductase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=msrA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16132041	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948734	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013804	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A744	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11433	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11433	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948734	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001397	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1403	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	482	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	801	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	446	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
msrA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4440179..4440219 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4219 (EcoliWiki Page)	NCBI GEO profiles for msrA
microarray	GenExpDB:b4219 (EcoliWiki Page)	Summary of data for msrA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4439684..4440038) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ09; Well:F7^[7]
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Notes

Accessions Related to msrA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11433	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1403	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4219
EcoGene	EcoGene:EG11433	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001397	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013804	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000011685 (score: 1.000; bootstrap: 100%) ENSANGP00000011803 (score: 0.989)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000015859 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT5G07470 (score: 1.000; bootstrap: 95%) AT5G61640 (score: 0.755) AT4G25130 (score: 0.556) AT5G07460 (score: 0.513)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000036759 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00033957 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00018393 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000011986 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000008363 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-050309-123 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0217823 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0000565 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000026814 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000313921 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000012283 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000011056 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:106916 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q9AY51 (score: 1.000; bootstrap: 93%) Q7XC38 (score: 1.000; bootstrap: 94%) Q7XUP7 (score: 0.392) Q706H9 (score: 0.371) Q7XUP6 (score: 0.317)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000034213 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000016616 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YER042W (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC29E65c (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000152470 (score: 1.000; bootstrap: 100%) NEWSINFRUP00000158962 (score: 0.158)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00038622001 (score: 1.000; bootstrap: 99%) GSTENP00038596001 (score: 0.805)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000001551 (score: 1.000; bootstrap: 99%) ENSXETP00000044576 (score: 0.199)	From Inparanoid:20070104
Shigella flexneri	MSRA	From SHIGELLACYC
E. coli O157	MSRA	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR10173:SF6
Superfamily (EcoliWiki Page)	SUPERFAMILY:55068
Pfam (EcoliWiki Page)	PF01625 Peptide methionine sulfoxide reductase
EcoCyc	EcoCyc:EG11433	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11433	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001397	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1403	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013804	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

msrA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to msrA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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