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Expression|

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Standard Name	mscS
Gene Synonym(s)	ECK2920, b2924, JW2891, yggB^[1], yggB
Product Desc.	mechanosensitive channel MscS^[2]^[3] Mechanosensitive channel protein, intermediate conductance; heptameric^[4]
Product Synonyms(s)	mechanosensitive channel^[1], B2924^[2]^[1], YggB^[2]^[1], MscS^[2]^[1] , ECK2920, JW2891, yggB, b2924
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mscS^[2]
Regulation/Activity
Quick Links
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Notes

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HT_Cmplx47_Mem: MscS+YiaF. mscS, kefA, yjeP, ybiO, ybdG and ynaI are paralogs.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mscS
Mnemonic	mechanosensitive channel Mechanosensitive channel
Synonyms	ECK2920, b2924, JW2891, yggB^[1], yggB
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	66.1 minutes	MG1655: 3067829..3066969 <gbrowseImage> name=NC_000913:3066969..3067829 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 2954117..2954977 <gbrowseImage> name=NC_012759:2954117..2954977 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3068463..3067603 <gbrowseImage> name=NC_007779:3067603..3068463 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3161699..3160839 <gbrowseImage> name=NC_010473:3160839..3161699 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔmscS (Keio:JW2891)	deletion	deletion		PMID:16738554	Shigen CGSC10242^[5]
mscSV40C,G,N		V40C,G,N	No detectable phenotype			seeded from UniProt:P0C0S1
mscSV40D,K		V40D,K	Normal growth stops, without cell death, due to increased membrane permeability to potassium ions and protons (permeability tested only for D substitutions)			seeded from UniProt:P0C0S1
mscSISFPYPQMDVNFKRVKEDKAA266HHHHHHLE		ISFPYPQMDVNFKRVKEDKAA266HHHHHHLE	Normal levels of channels are expressed; they recover more slowly than wild-type cells after desensitization			seeded from UniProt:P0C0S1
mscSS58C		S58C	Readily forms disulfides with cross-linkers, suggesting that individual S-58 are only 3 Angstroms apart in the closed state, versus 33 Angstroms apart in the open state crystal structure			seeded from UniProt:P0C0S1
mscSISFPYPQMDVNFKRVKEDKAA266LE		ISFPYPQMDVNFKRVKEDKAA266LE	Fewer channels present in the membrane, they require slightly more pressure to open and do not recover after desensitization			seeded from UniProt:P0C0S1
mscSS267C		S267C	Provides biochemical evidence for heptameric structure upon cross-linking			seeded from UniProt:P0C0S1
ΔmscS775::kan				PMID:16738554	CGSC:101956
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2891	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGAAGATTTGAATGTTGTCGA Primer 2:CCgGCAGCTTTGTCTTCTTTCAC
6C5	Kohara Phage	Genobase	PMID:3038334
recD1901::Tn10	Linked marker	CAG12135 = CGSC7429^[5]	est. P1 cotransduction: % ^[6]
speA210::Tn10	Linked marker	CAG12168 = CGSC7431^[5]	est. P1 cotransduction: 51% ^[6] Synonyms:zgd-210::Tn10, zgf-210::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11160	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11160	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001143	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947416	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1149	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009595	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MscS
Synonyms	mechanosensitive channel^[1], B2924^[2]^[1], YggB^[2]^[1], MscS^[2]^[1] , ECK2920, JW2891, yggB, b2924
Product description	mechanosensitive channel MscS^[2]^[3] Mechanosensitive channel protein, intermediate conductance; heptameric^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006811	ion transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0406	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008381	mechanically-gated ion channel activity	PMID:12080120	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	PMID:12080120	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	PMID:2480919	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0055085	transmembrane transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006685	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 3 predicted transmembrane domains	Daley et al. (2005) ^[7]
Inner Membrane		PMID:12767977, PMID:12015316, PMID:10202137, PMID:16079137	EchoLocation:mscS
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MEDLNVVDSI NGAGSWLVAN QALLLSYAVN IVAALAIIIV GLIIARMISN AVNRLMISRK IDATVADFLS ALVRYGIIAF TLIAALGRVG VQTASVIAVL GAAGLAVGLA LQGSLSNLAA GVLLVMFRPF RAGEYVDLGG VAGTVLSVQI FSTTMRTADG KIIVIPNGKI IAGNIINFSR EPVRRNEFII GVAYDSDIDQ VKQILTNIIQ SEDRILKDRE MTVRLNELGA SSINFVVRVW SNSGDLQNVY WDVLERIKRE FDAAGISFPY PQMDVNFKRV KEDKAA
Length	286
Mol. Wt	30.896 kDa
pI	8.6 (calculated)
Extinction coefficient	25,440 (calc based on 6 Y, 3 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	70..269	PF00924 Mechanosensitive ion channel	PMID:19920124
Domain	14..66	PF05552 Conserved TM helix	PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mscS taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130825	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947416	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009595	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0C0S1	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11160	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11160	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947416	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001143	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1149	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	4271	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	2927	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	2193	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mscS
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3067809..3067849 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2924 (EcoliWiki Page)	NCBI GEO profiles for mscS
microarray	GenExpDB:b2924 (EcoliWiki Page)	Summary of data for mscS from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3067727..3068240) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ06; Well:E3^[8]
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Notes

Accessions Related to mscS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11160	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1149	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2924
EcoGene	EcoGene:EG11160	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001143	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009595	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YGGB	From SHIGELLACYC
E. coli O157	YGGB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00924 Mechanosensitive ion channel
Superfamily (EcoliWiki Page)	SUPERFAMILY:50182
Superfamily (EcoliWiki Page)	SUPERFAMILY:82689
Superfamily (EcoliWiki Page)	SUPERFAMILY:82861
Pfam (EcoliWiki Page)	PF05552 Conserved TM helix
EcoCyc	EcoCyc:EG11160	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11160	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001143	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1149	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009595	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

mscS:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mscS Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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