PMID:2480919
| Citation |
Berrier, C, Coulombe, A, Houssin, C and Ghazi, A (1989) A patch-clamp study of ion channels of inner and outer membranes and of contact zones of E. coli, fused into giant liposomes. Pressure-activated channels are localized in the inner membrane. FEBS Lett. 259:27-32 |
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| Abstract |
Inner and outer membranes of Escherichia coli and contact zones were isolated and fused separately with giant liposomes amenable to patch-clamp recording. Different types of large pressure-activated channels were localized in the inner membrane fraction which also contained smaller, pressure-insensitive channels. The outer membrane contained pressure-insensitive channels with large conductances and long opening and closing times which are likely to be porins. Large channels were also observed in contact zones. |
| Links | |
| Keywords |
Cell Fractionation; Cell Membrane/physiology; Electric Conductivity; Escherichia coli/physiology; Escherichia coli/ultrastructure; Hydrostatic Pressure; Ion Channels/physiology; Liposomes; Membrane Potentials |
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