mscS:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
mscS |
|---|---|
| Mnemonic |
mechanosensitive channel Mechanosensitive channel |
| Synonyms |
ECK2920, b2924, JW2891, yggB[1], yggB |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
66.1 minutes |
MG1655: 3067829..3066969 |
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NC_012759: 2954117..2954977 |
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W3110 |
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W3110: 3068463..3067603 |
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DH10B: 3161699..3160839 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔmscS (Keio:JW2891) |
deletion |
deletion |
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mscSV40C,G,N |
V40C,G,N |
No detectable phenotype |
seeded from UniProt:P0C0S1 | ||||
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mscSV40D,K |
V40D,K |
Normal growth stops, without cell death, due to increased membrane permeability to potassium ions and protons (permeability tested only for D substitutions) |
seeded from UniProt:P0C0S1 | ||||
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mscSISFPYPQMDVNFKRVKEDKAA266HHHHHHLE |
ISFPYPQMDVNFKRVKEDKAA266HHHHHHLE |
Normal levels of channels are expressed; they recover more slowly than wild-type cells after desensitization |
seeded from UniProt:P0C0S1 | ||||
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mscSS58C |
S58C |
Readily forms disulfides with cross-linkers, suggesting that individual S-58 are only 3 Angstroms apart in the closed state, versus 33 Angstroms apart in the open state crystal structure |
seeded from UniProt:P0C0S1 | ||||
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mscSISFPYPQMDVNFKRVKEDKAA266LE |
ISFPYPQMDVNFKRVKEDKAA266LE |
Fewer channels present in the membrane, they require slightly more pressure to open and do not recover after desensitization |
seeded from UniProt:P0C0S1 | ||||
|
mscSS267C |
S267C |
Provides biochemical evidence for heptameric structure upon cross-linking |
seeded from UniProt:P0C0S1 | ||||
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ΔmscS775::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2891 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGAAGATTTGAATGTTGTCGA Primer 2:CCgGCAGCTTTGTCTTCTTTCAC | |
|
Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: % [6] | ||
|
Linked marker |
est. P1 cotransduction: 51% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
