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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	mppA
Gene Synonym(s)	ECK1326, b1329, JW1322, ynaH^[1], ynaH
Product Desc.	periplasmic murein tripeptide binding protein^[2]^[3]; Component of Murein Tripeptide ABC Transporter^[3] Murein tripeptide/heme transport, periplasmic binding protein; L-ala-gamma-D-glutamyl-meso-DAP binding protein; feeds into the Dpp (heme)and Opp (murein peptide) transport systems^[4]
Product Synonyms(s)	murein tripeptide (L-ala-gamma-D-glutamyl-meso-DAP) transporter subunit^[1], periplasmic-binding component of ABC superfamily^[1], B1329^[2]^[1], YnaH^[2]^[1], MppA^[2]^[1] , ECK1326, JW1322, ynaH, b1329
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mppA^[2]
Regulation/Activity
Quick Links
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Notes

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E. coli does not normally use exogeneous heme, but heme can be utilized if a foreign OM heme receptor is expressed in E. coli; heme and tripeptides compete for the same binding site; DppA is an alternative heme periplasmic binding protein; MppA or DppA is required for the uptake of 5-aminolevulinic acid (Letoffe, 2006).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mppA
Mnemonic	Murein peptide permease
Synonyms	ECK1326, b1329, JW1322, ynaH^[1], ynaH
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	29.99 minutes	MG1655: 1391251..1392864 <gbrowseImage> name=NC_000913:1391251..1392864 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1391596..1393209 <gbrowseImage> name=NC_012967:1391596..1393209 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1282100..1283713 <gbrowseImage> name=NC_012759:1282100..1283713 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1394941..1396554 <gbrowseImage> name=NC_007779:1394941..1396554 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1480647..1482260 <gbrowseImage> name=NC_010473:1480647..1482260 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1391317	Edman degradation	PMID:9495761
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔmppA (Keio:JW1322)	deletion	deletion			PMID:16738554	Shigen CGSC9183^[5]
ΔmppA767::kan					PMID:16738554	CGSC:101936
dppA::Kan mppA::Cm			Auxotrophies	Abolished heme utilization.		PMID:16905647	See table 1.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1322	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAGCACTCTGTTTCAGTCAC Primer 2:CCATGCTTCACAATATACATAGT
6B4	Kohara Phage	Genobase	PMID:3038334
3G3	Kohara Phage	Genobase	PMID:3038334
zcj-233::Tn10	Linked marker	CAG12028 = CGSC7373^[5]	est. P1 cotransduction: 43% ^[6] Synonyms:zci-233::Tn10
recT3061::Tn10	Linked marker	CAG12081 = CGSC7375^[5]	est. P1 cotransduction: 50% ^[6] Synonyms:zcj-3061::Tn10, zda-3061::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6665	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13376	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003260	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945951	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3158	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004460	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MppA
Synonyms	murein tripeptide (L-ala-gamma-D-glutamyl-meso-DAP) transporter subunit^[1], periplasmic-binding component of ABC superfamily^[1], B1329^[2]^[1], YnaH^[2]^[1], MppA^[2]^[1] , ECK1326, JW1322, ynaH, b1329
Product description	periplasmic murein tripeptide binding protein^[2]^[3]; Component of Murein Tripeptide ABC Transporter^[3] Murein tripeptide/heme transport, periplasmic binding protein; L-ala-gamma-D-glutamyl-meso-DAP binding protein; feeds into the Dpp (heme)and Opp (murein peptide) transport systems^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005215	transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000914	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042939	tripeptide transport	PMID: 9495761	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0015031	protein transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0653	P	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	PMID: 9495761	IDA: Inferred from Direct Assay		C		complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of Murein Tripeptide ABC Transporter	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
periplasm
Periplasm	PMID:9495761	EchoLocation:mppA
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSVRGKLMKH SVSVTCCALL VSSISLSYAA EVPSGTVLAE KQELVRHIKD EPASLDPAKA VGLPEIQVIR DLFEGLVNQN EKGEIVPGVA TQWKSNDNRI WTFTLRDNAK WADGTPVTAQ DFVYSWQRLV DPKTLSPFAW FAALAGINNA QAIIDGKATP DQLGVTAVDA HTLKIQLDKP LPWFVNLTAN FAFFPVQKAN VESGKEWTKP GNLIGNGAYV LKERVVNEKL VVVPNTHYWD NAKTVLQKVT FLPINQESAA TKRYLAGDID ITESFPKNMY QKLLKDIPGQ VYTPPQLGTY YYAFNTQKGP TADQRVRLAL SMTIDRRLMT EKVLGTGEKP AWHFTPDVTA GFTPEPSPFE QMSQEELNAQ AKTLLSAAGY GPQKPLKLTL LYNTSENHQK IAIAVASMWK KNLGVDVKLQ NQEWKTYIDS RNTGNFDVIR ASWVGDYNEP STFLTLLTST HSGNISRFNN PAYDKVLAQA STENTVKARN ADYNAAEKIL MEQAPIAPIY QYTNGRLIKP WLKGYPINNP EDVAYSRTMY IVKH
Length	544
Mol. Wt	60.672 kDa
pI	9.1 (calculated)
Extinction coefficient	102,790 - 103,040 (calc based on 21 Y, 13 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-22	UniProt Manual:Signal Peptides	UniProt:P77348
Domain	77..457	PF00496 Bacterial extracellular solute-binding proteins, family 5 Middle		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mppA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111250	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945951	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004460	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P77348	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6665	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13376	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945951	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003260	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3158	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	3.68E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	461	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	202	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	225	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mppA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1391231..1391271 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1329 (EcoliWiki Page)	NCBI GEO profiles for mppA
microarray	GenExpDB:b1329 (EcoliWiki Page)	Summary of data for mppA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to mppA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6665	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3158	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1329
EcoGene	EcoGene:EG13376	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003260	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004460	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000028369 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	S1424	From SHIGELLACYC
E. coli O157	Z2438	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:53850
Pfam (EcoliWiki Page)	PF00496 Bacterial extracellular solute-binding proteins, family 5 Middle
EcoCyc	EcoCyc:G6665	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13376	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003260	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3158	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004460	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

mppA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mppA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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