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Standard Name	modA
Gene Synonym(s)	ECK0752, b0763, JW0746^[1], JW0746
Product Desc.	ModA^[2]^[3]; Component of molybdate ABC transporter^[2]^[3] Molybdate uptake; chlorate resistance; periplasmic molybdate binding protein^[4]
Product Synonyms(s)	molybdate transporter subunit^[1], periplasmic-binding component of ABC superfamily^[1], B0763^[2]^[1], ModA^[2]^[1] , ECK0752, JW0746, b0763
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): modABC^[2]
Regulation/Activity
Quick Links
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Notes

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Flagellar regulon. The modA gene can be transcribed in vitro with sigma28 (FliA) holoenzyme (Yu, 2006).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	modA
Mnemonic	Molybdenum
Synonyms	ECK0752, b0763, JW0746^[1], JW0746
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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	17.12 minutes	MG1655: 794312..795085 <gbrowseImage> name=NC_000913:794312..795085 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 775718..776491 <gbrowseImage> name=NC_012967:775718..776491 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 697280..698053 <gbrowseImage> name=NC_012759:697280..698053 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 795511..796284 <gbrowseImage> name=NC_007779:795511..796284 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 848240..849013 <gbrowseImage> name=NC_010473:848240..849013 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	794384	Edman degradation	PMID:8576221
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔmodA (Keio:JW0746)	deletion	deletion	PMID:16738554	Shigen CGSC8807^[5]
modA::Tn5KAN-2 (FB20234)	Insertion at nt 340 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20234	does not contain pKD46
ΔmodA735::kan			PMID:16738554	CGSC:101910
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0746	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCTCGTAAATGGTTGAACTT Primer 2:CCCTTGATTGTAAATCCGTAACG
10F3	Kohara Phage	Genobase	PMID:3038334
nadA57::Tn10	Linked marker	CAG12147 = CGSC7351^[5]	est. P1 cotransduction: 59% ^[6]
zbh-29::Tn10	Linked marker	CAG18493 = CGSC7353^[5]	est. P1 cotransduction: 36% ^[6] Synonyms:zbi-29::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12427	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12427	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002283	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945364	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2325	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002601	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	ModA
Synonyms	molybdate transporter subunit^[1], periplasmic-binding component of ABC superfamily^[1], B0763^[2]^[1], ModA^[2]^[1] , ECK0752, JW0746, b0763
Product description	ModA^[2]^[3]; Component of molybdate ABC transporter^[2]^[3] Molybdate uptake; chlorate resistance; periplasmic molybdate binding protein^[4]
EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005215	transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006059	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006059	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015412	molybdate transmembrane-transporting ATPase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005950	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015689	molybdate ion transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005950	P	Seeded from EcoCyc (v14.0)	complete
	GO:0030151	molybdenum ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0500	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030288	outer membrane-bounded periplasmic space	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005950	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of molybdate ABC transporter	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
periplasm		From EcoCyc^[3]
Periplasm	PMID:8576221	EchoLocation:modA
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MARKWLNLFA GAALSFAVAG NALADEGKIT VFAAASLTNA MQDIATQFKK EKGVDVVSSF ASSSTLARQI EAGAPADLFI SADQKWMDYA VDKKAIDTAT RQTLLGNSLV VVAPKASVQK DFTIDSKTNW TSLLNGGRLA VGDPEHVPAG IYAKEALQKL GAWDTLSPKL APAEDVRGAL ALVERNEAPL GIVYGSDAVA SKGVKVVATF PEDSHKKVEY PVAVVEGHNN ATVKAFYDYL KGPQAAEIFK RYGFTIK
Length	257
Mol. Wt	27.364 kDa
pI	8.5 (calculated)
Extinction coefficient	32,430 (calc based on 7 Y, 4 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-24	UniProt Manual:Signal Peptides	UniProt:P37329
Domain	34..247	PF01547 Bacterial extracellular solute-binding protein		PMID:19920124^[7]
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=modA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128731	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945364	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002601	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P37329	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12427	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12427	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945364	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002283	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2325	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	4.79E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	769	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	462	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	491	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
modABC
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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:794292..794332 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0763 (EcoliWiki Page)	NCBI GEO profiles for modA
microarray	GenExpDB:b0763 (EcoliWiki Page)	Summary of data for modA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to modA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12427	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2325	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0763
EcoGene	EcoGene:EG12427	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002283	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002601	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000033611 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	MODA	From SHIGELLACYC
E. coli O157	MODA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01547 Bacterial extracellular solute-binding protein
Superfamily (EcoliWiki Page)	SUPERFAMILY:53850
EcoCyc	EcoCyc:EG12427	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12427	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002283	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2325	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002601	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

modA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to modA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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