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Standard Name	mltD
Gene Synonym(s)	ECK0211, b0211, JW5018, dniR, yafG^[1], yafG
Product Desc.	membrane-bound lytic murein transglycosylase D^[2]; MltD membrane-bound lytic murein transglycosylase D^[3] Membrane-bound lytic transglycosylase D, murein hydrolase; periplasmic OM-attached lipoprotein^[4]
Product Synonyms(s)	predicted membrane-bound lytic murein transglycosylase D^[1], B0211^[2]^[1], YafG^[2]^[1], MltD^[2]^[1], DniR^[2]^[1] , dniR, ECK0211, JW5018, yafG, b0211
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mltD^[2]
Regulation/Activity
Quick Links
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Notes

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MltD has been shown to be a murein hydrolase using zymography, to cause lysis or osmotically-stabilized spheroplasts when overproduced, and to be inhibited by bulgecin (Dijkstra A.J., Rudd, K.E., and Keck, W., A new member of the transglycosylase family of Escherichia coli displays a Gram-positive hydrolase motif, in "The soluble lytic transglycolsylase family of Escherichia coli", A.J. Dijkstra, pp. 85-101, Ph.D. thesis, University of Groningen, the Netherlands,1997). MltD has two Gram-positive peptidoglycan-binding motifs in the C-terminus. MltD has a central lysozyme-like domain. MltD should cleave the glycosidic bonds between the N-acetylmuramic acid and N-acetylglucosamine residues in murein. First 15 aa are a type II signal peptide. The proposed DniR function as a regulator of hexaheme nitrite reductase is probably artifactual.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mltD
Mnemonic	Membrane-bound lytic transglycosylase D
Synonyms	ECK0211, b0211, JW5018, dniR, yafG^[1], yafG
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	5.01 minutes	MG1655: 233955..232597 <gbrowseImage> name=NC_000913:232597..233955 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 236879..235521 <gbrowseImage> name=NC_012967:235521..236879 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 232596..233954 <gbrowseImage> name=NC_012759:232596..233954 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 233955..232597 <gbrowseImage> name=NC_007779:232597..233955 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 208059..206701 <gbrowseImage> name=NC_010473:206701..208059 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
mltD(del) (Keio:JW5018)	deletion	deletion	PMID:16738554	Shigen CGSC11132^[5]
dniR::Tn5KAN-2 (FB20047)	Insertion at nt 1174 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20047	does not contain pKD46
mltD730(del)::kan			PMID:16738554	CGSC:101886
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5018	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAGGCAAAAGCGATATTACT Primer 2:CCGGAATCTGGCATGTTGTTGTT
7D5	Kohara Phage	Genobase	PMID:3038334
yafC502::Tn10	Linked marker	CAG18436 = CGSC7328^[5]	est. P1 cotransduction: 98% ^[6] Synonyms:zae-502::Tn10
proA81::Tn10	Linked marker	CAG18447 = CGSC7330^[5]	est. P1 cotransduction: 35% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10246	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10246	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945694	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000240	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0242	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000704	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MltD
Synonyms	predicted membrane-bound lytic murein transglycosylase D^[1], B0211^[2]^[1], YafG^[2]^[1], MltD^[2]^[1], DniR^[2]^[1] , dniR, ECK0211, JW5018, yafG, b0211
Product description	membrane-bound lytic murein transglycosylase D^[2]; MltD membrane-bound lytic murein transglycosylase D^[3] Membrane-bound lytic transglycosylase D, murein hydrolase; periplasmic OM-attached lipoprotein^[4]
EC number (for enzymes)	3.2.1.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0009279	cell outer membrane				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0007047	cellular cell wall organization	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0961	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016829	lyase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0456	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016837	carbon-oxygen lyase activity, acting on polysaccharides	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.2.2	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016998	cell wall macromolecule catabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002482	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016998	cell wall macromolecule catabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018392	P	Seeded from EcoCyc (v14.0)	complete
	GO:0031225	anchored to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9901	C	Seeded from EcoCyc (v14.0)	complete
	GO:0051353	positive regulation of oxidoreductase activity	PMID:1663890	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0008933	lytic transglycosylase activity	PMID:10843862	NAS: Non-traceable Author Statement		F		complete
	GO:0016020	membrane	PMID:10843862	NAS: Non-traceable Author Statement		C		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	guaC		PMID:19402753	MALDI(Z-score):37.358916
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
membrane		From EcoCyc^[3]
Outer membrane	PMID:15174133	EchoLocation:mltD
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKAKAILLAS VLLVGCQSTG NVQQHAQSLS AAGQGEAAKF TSQARWMDDG TSIAPDGDLW AFIGDELKMG IPENDRIREQ KQKYLRNKSY LHDVTLRAEP YMYWIAGQVK KRNMPMELVL LPIVESAFDP HATSGANAAG IWQIIPSTGR NYGLKQTRNY DARRDVVAST TAALNMMQRL NKMFDGDWLL TVAAYNSGEG RVMKAIKTNK ARGKSTDFWS LPLPQETKQY VPKMLALSDI LKNSKRYGVR LPTTDESRAL ARVHLSSPVE MAKVADMAGI SVSKLKTFNA GVKGSTLGAS GPQYVMVPKK HADQLRESLA SGEIAAVQST LVADNTPLNS RVYTVRSGDT LSSIASRLGV STKDLQQWNK LRGSKLKPGQ SLTIGAGSSA QRLANNSDSI TYRVRKGDSL SSIAKRHGVN IKDVMRWNSD TANLQPGDKL TLFVKNNNMP DS
Length	452
Mol. Wt	49.418 kDa
pI	10.2 (calculated)
Extinction coefficient	61,880 - 62,005 (calc based on 12 Y, 8 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-15	UniProt Manual:Signal Peptides	UniProt:P0AEZ7
Domain	343..385	PF01476 LysM domain		PMID:19920124
Domain	402..443	PF01476 LysM domain		PMID:19920124
Domain	102..218	PF01464 Transglycosylase SLT domain		PMID:19920124
Domain	1..92	PF06474 MLTD_N		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mltD taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128198	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945694	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000704	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AEZ7	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10246	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10246	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945694	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000240	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0242	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	0.772+/-0.048	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
Protein	E. coli K-12 MG1655	761	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	644	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	355	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mltD
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:233935..233975 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0211 (EcoliWiki Page)	NCBI GEO profiles for mltD
microarray	GenExpDB:b0211 (EcoliWiki Page)	Summary of data for mltD from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (233858..234048) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ03; Well:H9^[7]
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Notes

Accessions Related to mltD Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10246	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0242	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0211
EcoGene	EcoGene:EG10246	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000240	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000704	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	DNIR	From SHIGELLACYC
E. coli O157	DNIR	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01476 LysM domain
Pfam (EcoliWiki Page)	PF01476 LysM domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:53955
Superfamily (EcoliWiki Page)	SUPERFAMILY:54106
Superfamily (EcoliWiki Page)	SUPERFAMILY:54106
Panther (EcoliWiki Page)	PTHR21666:SF47
Pfam (EcoliWiki Page)	PF01464 Transglycosylase SLT domain
Pfam (EcoliWiki Page)	PF06474 MLTD_N
EcoCyc	EcoCyc:EG10246	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10246	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000240	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0242	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000704	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

mltD:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mltD Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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