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Standard Name	minC
Gene Synonym(s)	ECK1164, b1176, JW1165, minB^[1], minB
Product Desc.	cell division inhibitor of the MinC-MinD-MinE and DicB-MinC systems that regulate septum placement^[2]^[3] Inhibition of FtsZ ring polymerization; forms membrane-associated coiled arrays, more concentrated at the poles^[4]
Product Synonyms(s)	cell division inhibitor^[1], B1176^[2]^[1], MinB^[2]^[1], MinC^[2]^[1] , ECK1164, JW1165, minB, b1176
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): minCDE^[2]
Regulation/Activity
Quick Links
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Notes

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MinCD oscillates (migrates) between coating one half of the cell's membrane (minE ring to pole) to coating the other half every 40 secs, and this may help to identify the cell center.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	minC
Mnemonic	Minicell
Synonyms	ECK1164, b1176, JW1165, minB^[1], minB
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	26.39 minutes	MG1655: 1225303..1224608 <gbrowseImage> name=NC_000913:1224608..1225303 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1226241..1225546 <gbrowseImage> name=NC_012967:1225546..1226241 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1113329..1114024 <gbrowseImage> name=NC_012759:1113329..1114024 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1227657..1226962 <gbrowseImage> name=NC_007779:1226962..1227657 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1265544..1264849 <gbrowseImage> name=NC_010473:1264849..1265544 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔminC (Keio:JW1165)	deletion	deletion			PMID:16738554	Shigen CGSC9075^[5]
minCG10D		G10D		(in minC19; reduces affinity of minC for ftsZ)			Strain variation; seeded from UniProt:P18196
ΔminC765::kan					PMID:16738554	CGSC:101874
ftsK1::cat ΔminB			Growth Phenotype	minicell chains	PMID:9829960
minC28					PMID:8752322		reduced interaction with MinD according to 2 hybrid screen
minC	deletion		Sensitivity to	increases sensitivity to bicyclomycin	PMID:21357484		fig 2
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1165	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCAAACACGCCAATCGAGCT Primer 2:CCATTTAACGGTTGAACGGTCAA
4D1	Kohara Phage	Genobase	PMID:3038334
2A3	Kohara Phage	Genobase	PMID:3038334
zcf-117::Tn10	Linked marker	CAG18463 = CGSC7363^[5]	est. P1 cotransduction: 62% ^[6]
fadR13::Tn10	Linked marker	CAG18497 = CGSC7365^[5]	est. P1 cotransduction: 72% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10596	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10596	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000589	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945744	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0591	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003941	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MinC
Synonyms	cell division inhibitor^[1], B1176^[2]^[1], MinB^[2]^[1], MinC^[2]^[1] , ECK1164, JW1165, minB, b1176
Product description	cell division inhibitor of the MinC-MinD-MinE and DicB-MinC systems that regulate septum placement^[2]^[3] Inhibition of FtsZ ring polymerization; forms membrane-associated coiled arrays, more concentrated at the poles^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000917	barrier septum formation	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00267	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000917	barrier septum formation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013033	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000917	barrier septum formation	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0717	P	Seeded from EcoCyc (v14.0)	complete
	GO:0007049	cell cycle	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0131	P	Seeded from EcoCyc (v14.0)	complete
	GO:0042127	regulation of cell proliferation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007874	P	Seeded from EcoCyc (v14.0)	complete
	GO:0051301	cell division	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0132	P	Seeded from EcoCyc (v14.0)	complete
	GO:0051726	regulation of cell cycle	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00267	P	Seeded from EcoCyc (v14.0)	complete
	GO:0051726	regulation of cell cycle	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013033	P	Seeded from EcoCyc (v14.0)	complete
	GO:0051301	cell division	PMID:2137246	EXP: Inferred from Experiment		P		complete
	GO:0051302	regulation of cell division	PMID:2137246	EXP: Inferred from Experiment		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rplB	PMID:16606699	Experiment(s):EBI-1139075
Protein	nuoE	PMID:16606699	Experiment(s):EBI-1139075
Protein	smp	PMID:16606699	Experiment(s):EBI-1139075
Protein	rpsB	PMID:16606699	Experiment(s):EBI-1139075
Protein	slyD	PMID:16606699	Experiment(s):EBI-1139075
Protein	rpsD	PMID:16606699	Experiment(s):EBI-1139075
Protein	rpsC	PMID:16606699	Experiment(s):EBI-1139075
Protein	yhjH	PMID:16606699	Experiment(s):EBI-1139075
Protein	rplE	PMID:16606699	Experiment(s):EBI-1139075
Protein	MinD	PMID:8752322	2 hybrid screen
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSNTPIELKG SSFTLSVVHL HEAEPKVIHQ ALEDKIAQAP AFLKHAPVVL NVSALEDPVN WSAMHKAVSA TGLRVIGVSG CKDAQLKAEI EKMGLPILTE GKEKAPRPAP TPQAPAQNTT PVTKTRLIDT PVRSGQRIYA PQCDLIVTSH VSAGAELIAD GNIHVYGMMR GRALAGASGD RETQIFCTNL MAELVSIAGE YWLSDQIPAE FYGKAARLQL VENALTVQPL N
Length	231
Mol. Wt	24.775 kDa
pI	6.9 (calculated)
Extinction coefficient	16,960 - 17,335 (calc based on 4 Y, 2 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	125..230	PF03775 Septum formation inhibitor MinC, C-terminal domain	PMID:19920124
Domain	1..104	PF05209 Septum formation inhibitor MinC, N-terminal domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=minC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129139	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945744	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003941	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P18196	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10596	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10596	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945744	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000589	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0591	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.50E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	857	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	364	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	575	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
minCDE
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1225283..1225323 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1176 (EcoliWiki Page)	NCBI GEO profiles for minC
microarray	GenExpDB:b1176 (EcoliWiki Page)	Summary of data for minC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1225207..1225850) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ13; Well:A7^[7]
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Notes

Accessions Related to minC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10596	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0591	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1176
EcoGene	EcoGene:EG10596	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000589	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003941	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000041170 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	MINC	From SHIGELLACYC
E. coli O157	MINC	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03775 Septum formation inhibitor MinC, C-terminal domain
Pfam (EcoliWiki Page)	PF05209 Septum formation inhibitor MinC, N-terminal domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:63848
EcoCyc	EcoCyc:EG10596	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10596	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000589	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0591	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003941	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

minC:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to minC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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