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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	mepA
Gene Synonym(s)	ECK2322, b2328, JW2325^[1], JW2325
Product Desc.	MepA^[2]^[3]; Component of murein DD-endopeptidase, penicillin-insensitive^[3] Murein DD-endopeptidase, penicillin-insensitive^[4]
Product Synonyms(s)	murein DD-endopeptidase^[1], B2328^[2]^[1] , ECK2322, JW2325, b2328
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yfcL^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mepA
Mnemonic	Murein peptidase
Synonyms	ECK2322, b2328, JW2325^[1], JW2325
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	52.67 minutes	MG1655: 2444406..2443582 <gbrowseImage> name=NC_000913:2443582..2444406 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2389976..2389152 <gbrowseImage> name=NC_012967:2389152..2389976 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2329387..2330211 <gbrowseImage> name=NC_012759:2329387..2330211 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2451830..2451006 <gbrowseImage> name=NC_007779:2451006..2451830 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2536171..2535347 <gbrowseImage> name=NC_010473:2535347..2536171 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2443585	Edman degradation	PMID:2187143
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔmepA (Keio:JW2325)	deletion	deletion			PMID:16738554	Shigen CGSC9864^[5]
mepAH211A		H211A		Strongly reduces enzyme activity			seeded from UniProt:P0C0T5
mepAH113A		H113A		Strongly reduces enzyme activity			seeded from UniProt:P0C0T5
mepAD120A		D120A		Strongly reduces enzyme activity			seeded from UniProt:P0C0T5
mepAH209A		H209A		Strongly reduces enzyme activity			seeded from UniProt:P0C0T5
ΔmepA::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
ΔmepA736::kan					PMID:16738554	CGSC:101822
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2325	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAATAAAACCGCGATTGCGCT Primer 2:CCGATCACGTGCTCATCCAGTAG
12F12	Kohara Phage	Genobase	PMID:3038334
2G11	Kohara Phage	Genobase	PMID:3038334
2C9	Kohara Phage	Genobase	PMID:3038334
zfb-223::Tn10	Linked marker	CAG18484 = CGSC7406^[5]	est. P1 cotransduction: 7% ^[6] Synonyms:zej-223::Tn10
zfd-1::Tn10	Linked marker	CAG18467 = CGSC7408^[5]	est. P1 cotransduction: 11% ^[6] Synonyms:zfb-1::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10580	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10580	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000573	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946812	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0575	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007691	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MepA
Synonyms	murein DD-endopeptidase^[1], B2328^[2]^[1] , ECK2322, JW2325, b2328
Product description	MepA^[2]^[3]; Component of murein DD-endopeptidase, penicillin-insensitive^[3] Murein DD-endopeptidase, penicillin-insensitive^[4]
EC number (for enzymes)	3.4.99.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000270	peptidoglycan metabolic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01623	P	Seeded from EcoCyc (v14.0)	complete
	GO:0004222	metalloendopeptidase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01623	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004252	serine-type endopeptidase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005073	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006508	proteolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005073	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008237	metallopeptidase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0482	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008270	zinc ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0862	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030288	outer membrane-bounded periplasmic space	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005073	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01623	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of murein DD-endopeptidase, penicillin-insensitive	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
Periplasm		PMID:2187143	EchoLocation:mepA
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNKTAIALLA LLASSASLAA TPWQKITQPV PGSAQSIGSF SNGCIVGADT LPIQSEHYQV MRTDQRRYFG HPDLVMFIQR LSSQVSNLGM GTVLIGDMGM PAGGRFNGGH ASHQTGLDVD IFLQLPKTRW TSAQLLRPQA LDLVSRDGKH VVSTLWKPEI FSLIKLAAQD KDVTRIFVNP AIKQQLCLDA GTDRDWLRKV RPWFQHRAHM HVRLRCPADS LECEDQPLPP SGDGCGAELQ SWFEPPKPGT TKPEKKTPPP LPPSCQALLD EHVI
Length	274
Mol. Wt	30.136 kDa
pI	8.4 (calculated)
Extinction coefficient	35,980 - 36,730 (calc based on 2 Y, 6 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-19	UniProt Manual:Signal Peptides	UniProt:P0C0T5
Domain	35..274	PF03411 Penicillin-insensitive murein endopeptidase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mepA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130263	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946812	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007691	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0C0T5	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10580	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10580	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946812	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000573	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0575	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	625	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	273	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	491	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yfcL
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2444386..2444426 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2328 (EcoliWiki Page)	NCBI GEO profiles for mepA
microarray	GenExpDB:b2328 (EcoliWiki Page)	Summary of data for mepA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to mepA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10580	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0575	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2328
EcoGene	EcoGene:EG10580	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000573	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007691	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	MEPA	From SHIGELLACYC
E. coli O157	MEPA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:55166
Pfam (EcoliWiki Page)	PF03411 Penicillin-insensitive murein endopeptidase
EcoCyc	EcoCyc:EG10580	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10580	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000573	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0575	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007691	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

mepA:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mepA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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