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Standard Name	menB
Gene Synonym(s)	ECK2256, b2262, JW2257^[1], JW2257
Product Desc.	NAPHTHOATE-SYN^[2]^[3] 1,4-Dihydroxy-2-naphthoate synthase; menaquinone biosynthesis^[4]
Product Synonyms(s)	dihydroxynaphthoic acid synthetase^[1], B2262^[2]^[1], MenB^[2]^[1], DHNA synthetase^[2]^[1], O-succinylbenzoyl-CoA 1,4-dihydroxy-2-naphthoate-lyase (cyclizing)^[2]^[1] , ECK2256, JW2257, b2262
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): menBCE^[2], menFD-yfbB-menBCE^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	menB
Mnemonic	Menaquinone (vitamin K2)
Synonyms	ECK2256, b2262, JW2257^[1], JW2257
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	51.17 minutes	MG1655: 2374841..2373984 <gbrowseImage> name=NC_000913:2373984..2374841 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2323332..2322475 <gbrowseImage> name=NC_012967:2322475..2323332 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2259789..2260646 <gbrowseImage> name=NC_012759:2259789..2260646 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2381489..2380632 <gbrowseImage> name=NC_007779:2380632..2381489 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2465829..2464972 <gbrowseImage> name=NC_010473:2464972..2465829 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔmenB (Keio:JW2257)	deletion	deletion	PMID:16738554	Shigen
menB::Tn5KAN-2 (FB20729)	Insertion at nt 409 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20729	contains pKD46
menB::Tn5KAN-2 (FB20730)	Insertion at nt 409 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20730	does not contain pKD46
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2257	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATTTATCCTGATGAAGCAAT Primer 2:CCtGGATTCCGTTTGAATTTGCT
5H12	Kohara Phage	Genobase	PMID:3038334
zfa-723::Tn10	Linked marker	CAG12178 gyrA+ = CGSC7526^[5]	est. P1 cotransduction: 30% ^[6] Synonyms:zei-723::Tn10, zfa-723::Tn10 nnnThe original CAG12178 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).
zfb-223::Tn10	Linked marker	CAG18484 = CGSC7406^[5]	est. P1 cotransduction: 58% ^[6] Synonyms:zej-223::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11368	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11368	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001336	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946747	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1342	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007476	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MenB
Synonyms	dihydroxynaphthoic acid synthetase^[1], B2262^[2]^[1], MenB^[2]^[1], DHNA synthetase^[2]^[1], O-succinylbenzoyl-CoA 1,4-dihydroxy-2-naphthoate-lyase (cyclizing)^[2]^[1] , ECK2256, JW2257, b2262
Product description	NAPHTHOATE-SYN^[2]^[3] 1,4-Dihydroxy-2-naphthoate synthase; menaquinone biosynthesis^[4]
EC number (for enzymes)	4.1.3.36^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0008935	naphthoate synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010198	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008935	naphthoate synthase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.1.3.36	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009234	menaquinone biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010198	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009234	menaquinone biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0474	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008935	naphthoate synthase activity	PMID:1629162	IDA: Inferred from Direct Assay		F		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	hldD	PMID:15690043	Experiment(s):EBI-884495, EBI-891396
Protein	rpsN	PMID:15690043	Experiment(s):EBI-891396
Protein	nadE	PMID:16606699	Experiment(s):EBI-1142375
Protein	pepD	PMID:16606699	Experiment(s):EBI-1142375
Protein	rpsN	PMID:19402753	LCMS(ID Probability):99.6
Protein	rfaD	PMID:19402753	LCMS(ID Probability):99.6 MALDI(Z-score):38.730315
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MIYPDEAMLY APVEWHDCSE GFEDIRYEKS TDGIAKITIN RPQVRNAFRP LTVKEMIQAL ADARYDDNIG VIILTGAGDK AFCSGGDQKV RGDYGGYKDD SGVHHLNVLD FQRQIRTCPK PVVAMVAGYS IGGGHVLHMM CDLTIAADNA IFGQTGPKVG SFDGGWGASY MARIVGQKKA REIWFLCRQY DAKQALDMGL VNTVVPLADL EKETVRWCRE MLQNSPMALR CLKAALNADC DGQAGLQELA GNATMLFYMT EEGQEGRNAF NQKRQPDFSK FKRNP
Length	285
Mol. Wt	31.633 kDa
pI	6.4 (calculated)
Extinction coefficient	36,900 - 37,900 (calc based on 10 Y, 4 W, and 8 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	35..206	PF00378 Enoyl-CoA hydratase/isomerase family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=menB taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130197	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946747	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007476	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ABU0	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11368	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11368	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946747	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001336	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1342	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	2.11E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	22.182+/-0.139	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.089848308	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	3887	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1235	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1934	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
menBCE
menFD-yfbB-menBCE
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2374821..2374861 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2262 (EcoliWiki Page)	NCBI GEO profiles for menB
microarray	GenExpDB:b2262 (EcoliWiki Page)	Summary of data for menB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to menB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11368	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1342	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2262
EcoGene	EcoGene:EG11368	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001336	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007476	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT1G60550 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC2D109 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	MENB	From SHIGELLACYC
E. coli O157	MENB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00378 Enoyl-CoA hydratase/isomerase family
Superfamily (EcoliWiki Page)	SUPERFAMILY:52096
EcoCyc	EcoCyc:EG11368	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11368	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001336	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1342	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007476	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

menB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to menB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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