{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	marA
Gene Synonym(s)	ECK1524, b1531, JW5249, cfxB, norB, soxQ, inaR, nfxC^[1], nfxC
Product Desc.	MarA transcriptional activator^[2]; MarA transcriptional dual regulator^[3] Transcriptional activator for multiple antibiotic resistance; AraC family; resistance to tetracycline, chloramphenicol^[4]
Product Synonyms(s)	DNA-binding transcriptional dual activator of multiple antibiotic resistance^[1], B1531^[2]^[1], CfxB^[2]^[1], InaR^[2]^[1], NorB^[2]^[1], SoxQ^[2]^[1], NfxC^[2]^[1], MarA^[2]^[1] , cfxB, ECK1524, inaR, JW5249, nfxC, norB, soxQ, b1531
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): marRAB^[2], OP00264
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

Martin (2002) provide some evidence in support of the this alternative ATG start: native protein is slightly smaller than the engineered protein by Western blot. The spacing to the GAGG RBS is bad for the first ATG and excellent for the second ATG. MarA is unstable.^[4]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	marA
Mnemonic	Multiple antibiotic resistance
Synonyms	ECK1524, b1531, JW5249, cfxB, norB, soxQ, inaR, nfxC^[1], nfxC
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	34.86 minutes	MG1655: 1617598..1617981 <gbrowseImage> name=NC_000913:1617598..1617981 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1587340..1587723 <gbrowseImage> name=NC_012967:1587340..1587723 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1509657..1510040 <gbrowseImage> name=NC_012759:1509657..1510040 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1621288..1621671 <gbrowseImage> name=NC_007779:1621288..1621671 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1707223..1707606 <gbrowseImage> name=NC_010473:1707223..1707606 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
marA(del) (Keio:JW5249)	deletion	deletion			PMID:16738554	Shigen CGSC11269^[5]
marA::Tn5KAN-2 (FB20350)	Insertion at nt 248 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20350	contains pKD46
marA::Tn5KAN-2 (FB20351)	Insertion at nt 248 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20351	does not contain pKD46
marA752(del)::kan					PMID:16738554	CGSC:101762
marA1			Resistant to	provides multiple antibiotic resistance	PMID:7679383
soxQ1			Resistant to	expresses multiple antibiotic resistance (elevated resistance to redox agents- menadione- and antibiotics- chloramphenicol and nalidixic acid) and diminished rate of synthesis of OmpF	PMID:7679383 PMID:1648558		also showed increased resistance to ampicillin and tetracycline Constitutively over-expressed the micF RNA
cfxB1			Resistant to	provided increased resistance to ampicillin and tetracycline and constitutively over-expressed the micF RNA	PMID:7679383 PMID:1648558
marA1			Resistant to	Resistant to Menadione	PMID:1648558	strain: JHC1113	Table 2. The table also have allelic variations with the same phenotype.
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5249	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCCAGACGCAATACTGACGC Primer 2:CCGCTGTTGTAATGATTTAATGG
2H2	Kohara Phage	Genobase	PMID:3038334
2B2	Kohara Phage	Genobase	PMID:3038334
zdd-235::Tn10	Linked marker	CAG18461 = CGSC7378^[5]	est. P1 cotransduction: % ^[6] Synonyms:zdc-235::Tn10
zdi-925::Tn10	Linked marker	CAG12151 = CGSC7385^[5]	est. P1 cotransduction: % ^[6] Synonyms:zdh-925::Tn10
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11434	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11434	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001398	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947613	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1404	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005112	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MarA
Synonyms	DNA-binding transcriptional dual activator of multiple antibiotic resistance^[1], B1531^[2]^[1], CfxB^[2]^[1], InaR^[2]^[1], NorB^[2]^[1], SoxQ^[2]^[1], NfxC^[2]^[1], MarA^[2]^[1] , cfxB, ECK1524, inaR, JW5249, nfxC, norB, soxQ, b1531
Product description	MarA transcriptional activator^[2]; MarA transcriptional dual regulator^[3] Transcriptional activator for multiple antibiotic resistance; AraC family; resistance to tetracycline, chloramphenicol^[4]
EC number (for enzymes)
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012287	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003700	transcription factor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000005	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005622	intracellular	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000005	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006350	transcription	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0804	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000005	P	Seeded from EcoCyc (v14.0)	complete
	GO:0043565	sequence-specific DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000005	F	Seeded from EcoCyc (v14.0)	complete
	GO:0045449	regulation of transcription	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012287	P	Seeded from EcoCyc (v14.0)	complete
	GO:0045449	regulation of transcription	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0805	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046677	response to antibiotic	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0046	P	Seeded from EcoCyc (v14.0)	complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	OmpF	Reduced amounts of OmpF porin were found in chromosomal Mare mutants	PMID:2848006
RNA	micF	marA causes a posttranscriptional decrease in ompF mRNA and reduced amounts of OmpF by directly or indirectly increasing micF expression	PMID:2848006	strains where the micF locus had been deleted showed the need for micF for OmpF reduction
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTMSRRNTDA ITIHSILDWI EDNLESPLSL EKVSERSGYS KWHLQRMFKK ETGHSLGQYI RSRKMTEIAQ KLKESNEPIL YLAERYGFES QQTLTRTFKN YFDVPPHKYR MTNMQGESRF LHPLNHYNS
Length	129
Mol. Wt	15.416 kDa
pI	9.8 (calculated)
Extinction coefficient	21,430 (calc based on 7 Y, 2 W, and 0 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	18..59	PF00165 Bacterial regulatory helix-turn-helix proteins, AraC family	PMID:19920124
Domain	66..109	PF00165 Bacterial regulatory helix-turn-helix proteins, AraC family	PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=marA taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111290	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947613	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005112	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ACH5	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11434	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11434	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947613	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001398	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1404	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	1160	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	394	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	606	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
marRAB
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:1617578..1617618 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1531 (EcoliWiki Page)	NCBI GEO profiles for marA
microarray	GenExpDB:b1531 (EcoliWiki Page)	Summary of data for marA from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
edit table

Notes

Accessions Related to marA Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11434	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1404	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1531
EcoGene	EcoGene:EG11434	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001398	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005112	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	MARA	From SHIGELLACYC
E. coli O157	MARA	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00165 Bacterial regulatory helix-turn-helix proteins, AraC family
Pfam (EcoliWiki Page)	PF00165 Bacterial regulatory helix-turn-helix proteins, AraC family
Superfamily (EcoliWiki Page)	SUPERFAMILY:46689
Superfamily (EcoliWiki Page)	SUPERFAMILY:46689
EcoCyc	EcoCyc:EG11434	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11434	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001398	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1404	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005112	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

marA:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to marA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search