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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

map

Gene Synonym(s)

ECK0166, b0168, JW0163[1], JW0163, pepM

Product Desc.

methionine aminopeptidase[2][3]

Methionine aminopeptidase[4]

Product Synonyms(s)

methionine aminopeptidase[1], B0168[2][1], Map[2][1] , ECK0166, JW0163, pepM, b0168

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): dapD[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

map

Mnemonic

Methionine aminopeptidase

Synonyms

ECK0166, b0168, JW0163[1], JW0163, pepM

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

4.07 minutes 

MG1655: 189506..188712
<gbrowseImage> name=NC_000913:188712..189506 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 192348..191554
<gbrowseImage> name=NC_012967:191554..192348 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 188711..189505
<gbrowseImage> name=NC_012759:188711..189505 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 189506..188712
<gbrowseImage> name=NC_007779:188712..189506 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 163610..162816
<gbrowseImage> name=NC_010473:162816..163610 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

188715

Edman degradation

PMID:3027045


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

mapH178A

H178A

Reduces activity 50-fold

seeded from UniProt:P0AE18

mapH79A

H79A

Reduces activity 100000-fold

seeded from UniProt:P0AE18

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0163

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCTATCTCAATCAAGACCCC

Primer 2:CCTTCGTCGTGCGAGATTATCGC

21C8

Kohara Phage

Genobase

PMID:3038334

12D5

Kohara Phage

Genobase

PMID:3038334

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[5]

est. P1 cotransduction: 18% [6]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[5]

est. P1 cotransduction: 15% [6]
Synonyms:zae-502::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10570

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10570

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000563

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947882

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0565

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000571

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Map

Synonyms

methionine aminopeptidase[1], B0168[2][1], Map[2][1] , ECK0166, JW0163, pepM, b0168

Product description

methionine aminopeptidase[2][3]

Methionine aminopeptidase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0006457

protein folding

P

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001714

P

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002467

P

Seeded from EcoCyc (v14.0)

complete

GO:0050897

cobalt ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0170

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

deaD

PMID:15690043

Experiment(s):EBI-886162

Protein

recE

PMID:15690043

Experiment(s):EBI-886162

Protein

rplD

PMID:15690043

Experiment(s):EBI-886162

Protein

rplI

PMID:15690043

Experiment(s):EBI-886162

Protein

rplL

PMID:15690043

Experiment(s):EBI-886162

Protein

rplM

PMID:15690043

Experiment(s):EBI-886162

Protein

rplS

PMID:15690043

Experiment(s):EBI-886162

Protein

rplV

PMID:15690043

Experiment(s):EBI-886162

Protein

rplW

PMID:15690043

Experiment(s):EBI-886162

Protein

rpmB

PMID:15690043

Experiment(s):EBI-886162

Protein

rpmG

PMID:15690043

Experiment(s):EBI-886162

Protein

rpsB

PMID:15690043

Experiment(s):EBI-886162

Protein

rpsE

PMID:15690043

Experiment(s):EBI-886162

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-886162

Protein

rpsP

PMID:15690043

Experiment(s):EBI-886162

Protein

rpsT

PMID:15690043

Experiment(s):EBI-886162

Protein

uidR

PMID:15690043

Experiment(s):EBI-886162

Protein

yeaP

PMID:15690043

Experiment(s):EBI-886162

Protein

mdtB

PMID:15690043

Experiment(s):EBI-886162

Protein

yhbY

PMID:15690043

Experiment(s):EBI-886162

Protein

maa

PMID:15690043

Experiment(s):EBI-886162

Protein

gshB

PMID:16606699

Experiment(s):EBI-1135767

Protein

recE

PMID:19402753

LCMS(ID Probability):99.0

Protein

maa

PMID:19402753

LCMS(ID Probability):99.0

Protein

mdtB

PMID:19402753

LCMS(ID Probability):99.0

Protein

uidR

PMID:19402753

LCMS(ID Probability):99.0

Protein

yeaP

PMID:19402753

LCMS(ID Probability):99.0

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MAISIKTPED IEKMRVAGRL AAEVLEMIEP YVKPGVSTGE LDRICNDYIV NEQHAVSACL
GYHGYPKSVC ISINEVVCHG IPDDAKLLKD GDIVNIDVTV IKDGFHGDTS KMFIVGKPTI
MGERLCRITQ ESLYLALRMV KPGINLREIG AAIQKFVEAE GFSVVREYCG HGIGRGFHEE
PQVLHYDSRE TNVVLKPGMT FTIEPMVNAG KKEIRTMKDG WTVKTKDRSL SAQYEHTIVV
TDNGCEILTL RKDDTIPAII SHDE
Length

264

Mol. Wt

29.33 kDa

pI

5.9 (calculated)

Extinction coefficient

17,420 - 18,295 (calc based on 8 Y, 1 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0AE18

Domain

12..242

PF00557 Metallopeptidase family M24

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=map taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128161

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947882

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000571

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AE18

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10570

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10570

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947882

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000563

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0565

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

5.71E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

124.971+/-0.408

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

1.6372+/-0.16645

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by FISH

PMID:20671182

mRNA

Ecoli K-12

0.147984887

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

9464

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2117

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

4467

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

dapD

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:189486..189526 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0168 (EcoliWiki Page)

NCBI GEO profiles for map

microarray

GenExpDB:b0168 (EcoliWiki Page)

Summary of data for map from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (189427..189956) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ09; Well:E2[7]

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Notes

Accessions Related to map Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10570

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0565

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0168

EcoGene

EcoGene:EG10570

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000563

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000571

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000015437 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000002101 (score: 1.000; bootstrap: 75%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT2G45240 (score: 1.000; bootstrap: 100%)
  • AT1G13270 (score: 0.088)
  • AT4G37040 (score: 0.052)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000021303 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00030136 (score: 1.000; bootstrap: 100%)
  • WBGene00030134 (score: 0.058)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00003129 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000015153 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Danio rerio

  • ZDB-EST-050302-146 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-050626-49 (score: 0.996)
  • ZDB-GENE-050626-124 (score: 0.996)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0205763 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0039219 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA12422-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000019961 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000296411 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000014486 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000025913 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Mus musculus

  • MGI:1922874 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q6Z6H2 (score: 1.000; bootstrap: 99%)
  • Q7XPL5 (score: 0.291)
  • Q6Z4B5 (score: 0.213)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000027998 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000017579 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YLR244C (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPBC3E70 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000183071 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00010275001 (score: 1.000; bootstrap: 95%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000020516 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

MAP

From SHIGELLACYC

E. coli O157

MAP

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Panther (EcoliWiki Page)

PTHR10804:SF45

Superfamily (EcoliWiki Page)

SUPERFAMILY:55920

Pfam (EcoliWiki Page)

PF00557 Metallopeptidase family M24

PFAM (EcoliWiki Page)

PFAM:PF00557

EcoCyc

EcoCyc:EG10570

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10570

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000563

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0565

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000571

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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